Cell invasion and migration assays were performed using a Transwell insert (8 μm PET membrane; Corning Life Sciences). For the migration assay, 4 × 104 cells in medium containing 2% FBS along with ACY-241 or dimethyl sulphoxide were introduced into the upper wells, while medium containing 2% FBS and ACY-241 or dimethyl sulphoxide was inserted into the lower wells. In the invasion assay, Corning Matrigel Matrix was diluted in serum-free medium to a final concentration of 100 μg/ml, and 50 μl of the diluted Matrigel Matrix was carefully added to the center of each Transwell. Following gel formation of the Matrigel Matrix, cell seeding was performed using the same method as in the migration assay. After 36 h, non-invading cells were removed. The invading cells present in the lower surface of the membrane were fixed with methanol and stained with 0.1% crystal violet. Five different sections of the membrane were photographed using a microscope, and the cell counts were determined in these five areas to calculate the average.
Transwell insert 8 μm pet membrane
Manufactured by Corning
Sourced in United States
The Transwell insert (8 μm PET membrane) is a laboratory equipment product designed for cell culture studies. It features an 8-micron polyethylene terephthalate (PET) membrane that allows for the study of cell migration, invasion, and barrier function.
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Lab products found in correlation
2 protocols using transwell insert 8 μm pet membrane
1
Transwell Assay for Cell Invasion and Migration
2
Matrigel-Based Cell Invasion Assay
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Cell invasion was assessed using a Matrigel invasion assay. Briefly, diluted Matrigel matrix was carefully added to the center of each Transwell® insert (8 μm PET membrane, Corning, NY, USA) for invasion assays. Cells were starved of serum for 24 h and then seeded in triplicate. Lower chambers contained serum-free medium or medium supplemented with 20% FBS. The inserts were washed twice with PBS1X before fixing and staining in crystal violet solution for 15 min and then air-dried. The invaded and migrated cells were observed and imaged under a microscope. The bound crystal violet was eluted with 33% acetic acid and the eluent absorbance at 590 nm was measured.
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