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Anti nitrotyrosine primary antibody a 21285

Manufactured by Thermo Fisher Scientific
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The Anti-nitrotyrosine primary antibody (A-21285) is a laboratory tool used to detect and quantify nitrated proteins in samples. It specifically binds to nitrotyrosine residues, allowing researchers to analyze oxidative stress and nitrosative damage in their experiments.

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Lab products found in correlation

Ix2 ucb, by Olympus (2 mentions) Apoptag plus peroxidase in situ apoptosis kit, by Merck Group (2 mentions)

Close spelling variants of this product

Anti-nitrotyrosine antibody (3 citations) Anti-nitrotyrosine (2 citations)

2 protocols using anti nitrotyrosine primary antibody a 21285

1

Immunohistochemical Analysis of Nitrotyrosine

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All tissues were embedded in paraffin prior to 10 micron sectioning. Histology samples were stained by hematoxylin and eosin under standard protocols. Immunohistochemistry samples were processed using an anti-nitrotyrosine primary antibody (A-21285, Life Technologies Inc., NY, USA) at 1:500 dilution, with color development according to the protocol described in the DAKO LSB+ kit. Endogenous peroxidases were quenched prior to processing. TUNEL staining was performed according to the manufacturer instructions using the ApopTag Plus Peroxidase In Situ Apoptosis Kit (EMD Millipore, MA, USA). All images were acquired using an Olympus IX2-UCB (Olympus America Inc., PA, USA) inverted fluorescence microscope equipped with a Nuance (CRi Inc., MA, USA) hyperspectral camera capable of brightfield full color imaging.
Shuhendler A.J., Pu K., Cui L., Uetrecht J.P, & Rao J. (2014). Real-time imaging of oxidative and nitrosative stress in the liver of live animals for drug-toxicity testing. Nature biotechnology, 32(4), 373-380.
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2

Immunohistochemical Analysis of Nitrotyrosine

Check if the same lab product or an alternative is used in the 5 most similar protocols
All tissues were embedded in paraffin prior to 10 micron sectioning. Histology samples were stained by hematoxylin and eosin under standard protocols. Immunohistochemistry samples were processed using an anti-nitrotyrosine primary antibody (A-21285, Life Technologies Inc., NY, USA) at 1:500 dilution, with color development according to the protocol described in the DAKO LSB+ kit. Endogenous peroxidases were quenched prior to processing. TUNEL staining was performed according to the manufacturer instructions using the ApopTag Plus Peroxidase In Situ Apoptosis Kit (EMD Millipore, MA, USA). All images were acquired using an Olympus IX2-UCB (Olympus America Inc., PA, USA) inverted fluorescence microscope equipped with a Nuance (CRi Inc., MA, USA) hyperspectral camera capable of brightfield full color imaging.
Shuhendler A.J., Pu K., Cui L., Uetrecht J.P, & Rao J. (2014). Real-time imaging of oxidative and nitrosative stress in the liver of live animals for drug-toxicity testing. Nature biotechnology, 32(4), 373-380.
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