Logarithmic growth phase cells were digested with 0.25% trypsin and adjusted to 250 cells/mL. Cells (2 mL/well) were cultured in a 6-well plate at 37 °C and 5% CO 2 for 2-3 weeks, and the fresh medium was added every 3 days. Methanol was used to x the cells, and 1 ml of Giemsa working uid (48900, Sigma-Aldrich, Shanghai, China) was used to stain the cells for 30 min. After two washes with ultrapure water, lter paper was used to remove the water around the dish, and the cells were imaged by a camera (Eos RP, Canon, Japan).
Giemsa working uid
Manufactured by Merck Group
Sourced in China
Giemsa working fluid is a laboratory reagent used in microscopy and hematology. It is a staining solution that is commonly used to differentiate and identify different types of blood cells and other cellular structures. The Giemsa working fluid contains a combination of methylene blue, eosin, and azure dyes, which stain various cellular components, allowing for better visualization and analysis under a microscope.
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Lab products found in correlation
3 protocols using giemsa working uid
1
Quantitative Cell Staining Assay
2
Quantitative Cell Staining Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Logarithmic growth phase cells were digested with 0.25% trypsin and adjusted to 250 cells/mL. Cells (2 mL/well) were cultured in a 6-well plate at 37 °C and 5% CO 2 for 2-3 weeks, and the fresh medium was added every 3 days. Methanol was used to x the cells, and 1 ml of Giemsa working uid (48900, Sigma-Aldrich, Shanghai, China) was used to stain the cells for 30 min. After two washes with ultrapure water, lter paper was used to remove the water around the dish, and the cells were imaged by a camera (Eos RP, Canon, Japan).
3
Quantitative Cell Staining Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Logarithmic growth phase cells were digested with 0.25% trypsin and adjusted to 250 cells/mL. Cells (2 mL/well) were cultured in a 6-well plate at 37 °C and 5% CO 2 for 2-3 weeks, and the fresh medium was added every 3 days. Methanol was used to x the cells, and 1 ml of Giemsa working uid (48900, Sigma-Aldrich, Shanghai, China) was used to stain the cells for 30 min. After two washes with ultrapure water, lter paper was used to remove the water around the dish, and the cells were imaged by a camera (Eos RP, Canon, Japan).
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