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Ab256270

Manufactured by Abcam
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Ab256270 is a primary antibody product from Abcam. It is a polyclonal antibody that recognizes a specific target protein. The core function of this product is to detect the target protein in various applications, such as Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA).

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Lab products found in correlation

Ab195191, by Abcam (1 mentions) Cytoflex s, by Beckman Coulter (1 mentions) Ab283654, by Abcam (1 mentions) Facsaria 2, by BD (1 mentions) Kaluza analysis 2, by Beckman Coulter (1 mentions)

2 protocols using ab256270

1

Quantifying Cell Phenotypes by Flow Cytometry

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To determine the cell quantity of different phenotypes after indicated treatment, harvested cell suspensions were incubated Alexa Fluor® 488 mouse monoclonal antibody to CD86 (Abcam, ab256270) and Alexa Fluor® 488 rabbit monoclonal antibody to CD206 (Abcam, ab195191). Cells were washed, re‐suspended in FACS buffer, and detected by flow cytometer (CytoFLEX S, Beckman Coulter, USA). Non‐specific binding was assessed using isotype‐matched antibodies.
Li J., Zeng Q., Su W., Song M., Xie M, & Mao L. (2021). FBXO10 prevents chronic unpredictable stress‐induced behavioral despair and cognitive impairment through promoting RAGE degradation. CNS Neuroscience & Therapeutics, 27(12), 1504-1517.
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2

Multicolor Flow Cytometry of Immune Cells

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Cells were incubated with uorescently labeled Alexa Fluor® 488 anti-rat CD86 antibody (ab256270, Abcam) (1:100), APC anti-mannose receptor antibody (ab223961, Abcam) (1:100) and Anti-CD68 antibody (ab283654, Abcam) (1:100) for 30 min. FACS was performed with the BD FACS Aria II cytometer (Becton Dickinson), and the data were analyzed using Kaluza Analysis 2.1 (Beckman Coulter Life Sciences, USA).
Li H., Xiang Y., Zhu Z., Wang W., Jiang Z., Zhao M., Cheng S., Pan F., Liu D., Ho R., & Ho C.S.H. (2021). Rifaximin-Mediated Gut Microbiota Regulation Modulates the Polarization of Microglia During CUMS in Rat.
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