Mitochondrial complex I activity was measured using an immunocapture ELISA complex I enzyme activity assay kit, according to the manufacturer's protocol (Abcam, Cambridge, MA). For each age, a single experiment included liver and hippocampal samples, representing one biological sample of each sex/stress group. To ensure that all assays were in the linear range, three dilutions of mitochondrial protein (20–80 μg) from each group were plated per well. Measurements were done in duplicates that were averaged. Activity was expressed as change in absorbance/minute/ug protein.
Immunocapture elisa complex 1 enzyme activity assay kit
Manufactured by Abcam
The Immunocapture ELISA complex I enzyme activity assay kit is a laboratory tool designed to measure the activity of complex I, a key enzyme in the mitochondrial electron transport chain. The kit utilizes an immunocapture-based approach to selectively isolate and quantify the activity of complex I from biological samples.
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2 protocols using immunocapture elisa complex 1 enzyme activity assay kit
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Mitochondrial Complex I Activity Assay
2
Measuring Mitochondrial Complex I Activity
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Mitochondrial complex I activity was measured using an immunocapture ELISA complex I enzyme activity assay kit, according to the manufacturer's protocol (Abcam, Cambridge, MA). For each age, a single experiment included liver and hippocampal samples, representing one biological sample of each sex/stress group. To ensure that all assays were in the linear range, three dilutions of mitochondrial protein (20-80μg) from each group were plated per well. Measurements were done in duplicates that were averaged. Activity was expressed as change in absorbance/minute/ug protein.
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