Cells were grown in a six‐well plate to 80% confluence. Then, trypsin without EDTA was used to treat cells to obtain single cell suspension. For cell cycle analysis, single cell suspension (1 × 106 cells·mL−1) was fixed in 70% ice‐cold ethanol overnight, and then washed and stained in dark in propidium iodide/RNase staining buffer at room temperature for 30 min in accordance with the manufacturer’s instructions (catalog. no. KGA512; KeyGen, Nanjing, China). For apoptosis analysis, cell suspension (5 × 105 cells·mL−1) in 1 × binding buffer was stained with annexin V‐kFluor647 and propidium iodide in accordance with the manufacturer’s instructions (catalog. no. KGAV115; KeyGen). Three independent experiments were performed for statistical analysis.
Kgav115
Manufactured by Keygen Biotech
The KGAV115 is a high-performance analytical centrifuge designed for a wide range of laboratory applications. It features a powerful motor and a robust construction to ensure reliable and consistent performance. The KGAV115 can handle a variety of sample types and volumes, making it a versatile tool for researchers and scientists.
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Lab products found in correlation
2 protocols using kgav115
1
Cell Cycle and Apoptosis Analysis
2
Cell Cycle and Apoptosis Analysis
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Cells were grown in a six‐well plate to 80% confluence. Then, trypsin without EDTA was used to treat cells to obtain single‐cell suspension. For cell cycle analysis, single‐cell suspension (1 × 106 cells/mL) was fixed in 70% ice‐cold ethanol overnight, and then washed and stained in dark in PI/RNase staining buffer at room temperature for 30 minutes following the manufacturer's manual (KeyGen, KGA512). For apoptosis analysis, cell suspension (5 × 105 cells/mL) in 1× binding buffer was stained with Annexin V‐kFluor647 and PI following the manufacturer's manual (KeyGen, KGAV115). Three independent experiments were performed for statistical analysis.
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