Enhanced chemiluminescence ecl western blotting reagents

Flaccidoxide-13-acetate was isolated from cultured-type soft coral Sinularia gibberosa by Dr. Jui-Hsin Su. Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and phosphate-buffered saline (PBS), were purchased from Biowest (Nuaillé, France). Polyvinylidene difluoride (PVDF) membranes were purchased from Millipore (Bellerica, MA, USA). Protease inhibitor cocktail, dimethyl sulfoxide (DMSO), salubrinal, and goat anti-rabbit and horseradish peroxidase-conjugated immunoglobulin (Ig) G were obtained from Sigma (St. Louis, MO, USA). Cell extraction buffer was acquired from BioSource International (Camarillo, CA, USA). An annexin V-FITC/PI apoptosis detection kit was purchased from Pharmingen (San Diego, CA, USA). The enhanced chemiluminescence (ECL) Western blotting reagents were obtained from Pierce Biotechnology (Rockford, IL, USA). Cytochrome C releasing apoptosis assay kit was purchased form Biovision (Milpitas, CA, USA).

Many reagents, including Dulbecco’s modified Eagle’s medium (DMEM), McCoy’s 5a medium, trypsin-ethylenediaminetetraacetic acid, fetal bovine serum (FBS), and phosphate-buffered saline (PBS), were obtained from Biowest (Nuaillé, France). Polyvinylidene difluoride (PVDF) membranes, and goat anti-rabbit and horseradish peroxidase (HRP)-conjugated immunoglobulin (Ig) G were obtained from Millipore (Billerica, MA, USA). Protease inhibitor cocktail and DMSO were obtained from BioSource International (Camarillo, CA, USA). Cell extraction radioimmunoprecipitation assay (RIPA) buffer was obtained from TOOLS (TOOLS, Taiwan). Enhanced chemiluminescence (ECL) Western blotting reagents were obtained from Pierce Biotechnology (Rockford, IL, USA). Mouse anti-human β-actin antibodies were obtained from Sigma (St Louis, MO, USA). Rabbit anti-human FAK, mTOR, and p-mTOR antibodies were obtained from Epitomics (Burlingame, CA, USA). Rabbit anti-human TIMP-1, AKT, and p-AKT antibodies were obtained from ProteinTech Group (Chicago, IL, USA). Rabbit anti-human MMP-2, MMP-9, PI3K, p-PI3K, and EMT kit (#9782) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA).

Many reagents, including Dulbecco’s modified Eagle’s medium (DMEM), trypsin-ethylenediaminetetraacetic acid, fetal bovine serum (FBS), and phosphate-buffered saline (PBS), were obtained from Biowest (Nuaillé, France). The 2-D Quant Kit protein assay kit, immobilized pH gradient (IPG) buffer, and isoelectrofocusing strips were obtained from GE Healthcare (Buckinghamshire, UK). Polyvinylidene difluoride (PVDF) membranes, and goat anti-rabbit and horseradish peroxidase-conjugated immunoglobulin (Ig) G were obtained from Millipore (Billerica, MA, USA). Protease inhibitor cocktail, DMSO, ArA, CyA, TFZ were obtained from BioSource International (Camarillo, CA, USA). Cell extraction RIPA buffer was obtained from TOOLS (TOOLS, Taiwan). Enhanced chemiluminescence (ECL) western blotting reagents were obtained from Pierce Biotechnology (Rockford, IL, USA). Antibodies against PDIA3, 14-3-3 sigma, T-complex1, Stress-70, Annexin A2, and UQCRC2, were obtained from Epitomics (Burlingame, CA, USA). Antibodies against Endo G and AIF were obtained from ProteinTech Group (Chicago, IL, USA). Antibodies against pro-caspase 3, cleaved-caspase 3, pro-caspase 9, cleaved-caspase 9, cytochrome C, Bid, Bax, Bad, p-Bad, Bcl2, and Bcl-xL were obtained from Cell Signaling Technology (Danvers, MA, USA).