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Lysophosphatidylcholine 17 1 lpc 17 1

Manufactured by Avanti Polar Lipids

Lysophosphatidylcholine 17:1 (LPC 17:1) is a lipid molecule composed of a 17-carbon fatty acid chain and a phosphocholine headgroup. It is a member of the lysophosphatidylcholine class of lipids.

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2 protocols using lysophosphatidylcholine 17 1 lpc 17 1

1

Lipid Extraction for Mass Spectrometry

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HPLC grade water and LC-MS grade methanal were purchased from Acros Organics (New Jersey). Lysophosphatidylcholine 17:1 (LPC 17:1) was purchased from Avanti Polar Lipids (Alabaster, AL). The extraction solvent used in this study was composed of 1 μM LPC 17:1 in 9 : 1 MeOH : H2O (v/v). Fused silica capillaries (50 μm ID, 150 μm OD) were purchased from Polymicro Technologies (Phoenix, AZ). The MinuteWeld epoxy glue was purchased from J-B Weld company (Sulphur Springs, TX). A 2000 grit silicon carbide sandpaper (STARCKE, Germany) was used to polish the SLE-MFP sampling port.
4′′ fused-silica substrates with a thickness of 1 mm were obtained from Siegert Wafer GmbH (Aachen, Germany). Potassium hydroxide solution (45%, extra pure) and MeOH (HPLC grade) were purchased from Carl Roth GmbH & Co. KG (Karlsruhe, Germany). Dioctyl sulfosuccinate sodium salt (≥99%) was purchased from Sigma-Aldrich GmbH (Taufkirchen, Germany).
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2

Optimized Lipidomics Analysis Workflow

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HPLC grade water and LC-MS grade methanol were purchased from Acros Organics (NJ). Lysophosphatidylcholine 17:1 (LPC 17:1); Phosphatidylcholine 18:2 (Cis) (DLPC) (PC 18:2) and 18:1 (11-cis) (PC 18:1); phosphocholine 12:0–13:0 (PC 12:0–13:0) were obtained from Avanti Polar Lipids (Alabaster, AL). Supelco stainless-steel tubes with an outer diameter of 1/16 in. were acquired from MilliporeSigma. The ESI-L low concentration tune mix (G1969–85000, Agilent Technologies, CA) was used to calibrate the instrument.
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