MDA-MB-231 human triple negative breast cancer cells were obtained from American Type Culture Collection (ATCC) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin in a controlled environment (37°C and 5% CO2). Cell line authentication was performed by Short Tandem Repeat (STR) profiling at the University of Illinois Research Resource Center before use. Prior to experiments, cells were grown to 80% confluency and serum starved overnight prior to treatment with DETA/NO.
Mda mb 231 human triple negative breast cancer cells
Manufactured by American Type Culture Collection
MDA-MB-231 is a human triple negative breast cancer cell line. It is a widely used model for the study of breast cancer biology and preclinical drug testing.
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2 protocols using mda mb 231 human triple negative breast cancer cells
1
MDA-MB-231 Breast Cancer Cell Culture
2
Stable Overexpression of miR-766 in TNBC
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MDA-MB-231 human triple-negative breast cancer cells were obtained from the American Type Culture Collection (Manassas, VA). Human miR-766-overexpressing MDA-MB-231 cells (MB231_miR-766) were established by stable transfection using the full sequence of human miR-766. Briefly, MDA-MB-231 cells were transfected with the pcDNA3.1 plus pSUPER plasmid containing full sequence of human miR-766 using Promofectin (PromoKine, Heidelberg, Germany), according to the manufacturer's instruction. Stable transfected clones were established by selection with G418 (Sigma-Aldrich, St. Louis, MO) at a concentration of 500 µg/mL for 3 weeks. Control cells were transfected with the pcDNA3.1 vector only (MB231_Vector).
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