Terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling tunel kit

Antibodies to BMP2, SYCP3, MVH, γHis2A.X and MCM2 were obtained from Abcam (Cambridge, MA). Alexa-conjugated secondary antibodies were obtained from Invitrogen (Carlsbad, CA). Plastic embedding medium was obtained from Electron Microscopy Sciences (Hatfield, Pa). Tissue-Tek Optimal Cutting Temperature (OCT) compound was obtained from Sacura Finetek USA Inc. (Torrance, CA). Phenol red-free DMEM, linolenic acid, BSA, and fine chemicals were purchased from Sigma Chemical Company (St. Louis, MO). LDN 193,189 was purchased from Sigma Chemical Co. (St. Louis, MO). DMH1 was purchased from Tocris Bioscience (Bristol, UK). Human holo-transferrin was obtained from BD pharmaceuticals (San Jose, CA). Quantitative RT-PCR primers and probes were synthesized by Sigma Chemical Company. RNeasy mini kit and Taq DNA polymerase were from Qiagen, Inc. (Valencia, CA). The terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) kit was from Roche pharmaceuticals (Mannheim, Germany). All other molecular biology grade chemicals were obtained from Sigma Chemical Company or Fisher Scientific (Pittsburgh, PA).

Polyethylene glycol monomethyl ether (mPEG), L-phenylalanine (L-Phe), L-cysteine (L-Cys), and deuterated trifluoroacetic acid (TFA-d) were purchased from Sigma-Aldrich (Shanghai, PR China). The STP, DOX hydrochloride (DOX HCl), and GSH were obtained from Gill Biochemical Co., Ltd. (Shanghai, PR China). Cell culture products, including Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS), were provided by Gibco (USA). Penicillin and streptomycin were obtained from Huabei Pharmaceutical Co., Ltd. (Hebei, PR China). Sodium cyanoborohydride (NaBH3CN), 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) were purchased from Sigma-Aldrich (Shanghai, P. China). Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) kit was purchased from Roche Company (Mannheim, Germany). The purified deionized water was prepared by the Milli-Q plus system (Millipore Co., Billerica, MA, USA).