Fixed frozen slides were used for histology-based methods. Slides were stained with Cd68, F4/80, Cxcl4, Cd169, Cd11b, and SIGNR1. Secondary Alexa Fluor (AF)-conjugated antibodies were used. Slides were mounted in Fluoromount G with DAPI (Invitrogen). Images were taken using a three laser fluorescent microscope (Biorevo BZ-900 microscope with BZII Viewer software, Keyence) and processed in ImageJ.
Five total human samples were selected according to lack of pathological findings in sural nerve biopsies. The study received ethical approval by the ethical board of the University Clinic Leipzig, Germany. The staining was performed by the automated immunostainer Benchmark XT (Roche). The following antibodies were used: CD45, CD68, CD8, CD4, CD34, SMA/ACTA2, SOX10, and MBP.
Five total human samples were selected according to lack of pathological findings in sural nerve biopsies. The study received ethical approval by the ethical board of the University Clinic Leipzig, Germany. The staining was performed by the automated immunostainer Benchmark XT (Roche). The following antibodies were used: CD45, CD68, CD8, CD4, CD34, SMA/ACTA2, SOX10, and MBP.