HPLC was performed using Agilent 1200 HPLC with G1321A FD, and Eclipse AAA column (4.6×150 mm, 5 µm) with an excitation wavelength (λex) of 340 nm and an emission wavelength (λem) of 455 nm, and a column temperature of 40°C. The derivative liquid was compounded as follows: 5 mg o-Phthalaldehyde was dissolved in 120 µL dehydrated ethanol, and then added to 1 mL borate buffer (0.2 mol/L, pH 9.2) and 10 µL β-mercaptoethanol. Mobile phase A consisted of sodium borate buffer, methanol, and tetrahydrofuran (in a ratio of 400:95:5), and mobile phase B consisted of sodium acetate buffer solution (pH 7.2) and methanol (in a ratio of 120:380). The gradient elution procedure was 0–10 minutes, B (0%–63%), 10–12 minutes, B (63%), 12–17 minutes, B (100%), 17–18 minutes, B (100%–0%), and 18–21 minutes, B (0%). The flow rate was 0.8 mL min−1.
G1321a fd
Manufactured by Agilent Technologies
The G1321A FD is a fluorescence detector designed for liquid chromatography (LC) applications. It is capable of detecting fluorescent compounds in liquid samples.
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Lab products found in correlation
4 protocols using g1321a fd
1
HPLC Fluorescence Detection of OPA Derivatives
2
HPLC Separation and Quantification
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HPLC was performed using Agilent 1200 HPLC with G 1321 A FD and Eclipse AAA columns (4.6× 150 mm, 5 m) and column temperature of 40°C. Mobile phase A (formic acid: water = 1: 1000) and mobile phase B (acetonitrile). Gradient elution procedure is 0-70 minutes, B (0%-63%), 0-20 minutes, B (63%), 20-40 minutes, B (100%), 50 minutes, B (100%-0%), and 50-70 minutes, B (0%). The flow rate was 0.4 ml min−1.
3
HPLC Analysis of Organic Compounds
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HPLC was performed using Agilent 1200 HPLC with G1321A FD and Eclipse AAA column (4.6 × 150 mm, 5 μm) and a column temperature of 40°C: mobile phase A (formic acid : water = 1 : 1000) and mobile phase B (acetonitrile). The gradient elution procedure was 0–13 minutes, B (0%–63%), 0–8 minutes, B (63%), 9-10 minutes, B (100%), 11 minutes, B (100%–0%), and 12-13 minutes, B (0%). The flow rate was 0.4 ml min−1.
4
HPLC Analysis of Organic Compounds
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HPLC was performed using Agilent 1200 HPLC with G1321A FD, Eclipse AAA column (4.6×150 mm, 5 μm), and a column temperature of 40°C. Mobile phase A (formic acid: water =1:1000) and mobile phase B (acetonitrile). The gradient elution procedure was 0–13 minutes, B (0%–63%), 0–5 minutes, B (63%), 5-8 minutes, B (100%), 9 minutes, B (100%–0%), and 10-13 minutes, B (0%). The flow rate was 0.4 mL min−1.
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