Mouse anti rabbit hrp

Manufactured by Santa Cruz Biotechnology

Sourced in United Kingdom

Mouse anti-rabbit HRP is a secondary antibody conjugated with horseradish peroxidase (HRP). It is used to detect and visualize primary antibodies raised in rabbit.

Automatically generated - may contain errors

Lab products found in correlation

Goat anti mouse hrp, by Jackson ImmunoResearch (3 mentions) Mouse α gapdh, by Abcam (2 mentions) Rabbit α gapdh, by Cell Signaling Technology (2 mentions) Pou4f1, by Santa Cruz Biotechnology (2 mentions) Geldoc imager, by Jackson ImmunoResearch (2 mentions) Foxc1, by Cell Signaling Technology (2 mentions) Ab41902, by Abcam (1 mentions) Ab52617, by Abcam (1 mentions) Ab18180, by Abcam (1 mentions) Ecl western blotting kit, by Cytiva (1 mentions) Gapdh antibody, by Santa Cruz Biotechnology (1 mentions) Cell extraction buffer, by Thermo Fisher Scientific (1 mentions) Rabbit anti sting, by Cell Signaling Technology (1 mentions) Rabbit anti tbk1, by Cell Signaling Technology (1 mentions) Rabbit anti irf3, by Cell Signaling Technology (1 mentions) Mouse anti ha 11, by BioLegend (1 mentions) Nupage 4 12 bis tris precast protein gels, by Thermo Fisher Scientific (1 mentions) Anti mouse iggκ hrp, by Santa Cruz Biotechnology (1 mentions) Supersignal west femto maximum sensitivity substrate, by Thermo Fisher Scientific (1 mentions) Bcl 6, by BD (1 mentions)

7 protocols using mouse anti rabbit hrp

Cryogenic Preservation and Western Blot Analysis of Thoracic Aortic Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

The thoracic aortic tissue was put into cryogenic vials, which were cryopreserved immediately in liquid nitrogen and stored at −80°C for use after completion of all sample collections.
The thoracic arteries (length 1.5 cm) were dissected and used to analyse the protein levels using Western blotting. Lysates (10–30 µg protein) were loaded onto 10% SDS-PAGE gels and blotted onto a polyvinylidene difluoride membrane. After being blocked with 5% powdered skim milk for 2 hours in phosphate-buffered saline containing 0.1% Tween 20 (PBST), the membranes were incubated with ABCA1 antibody (ab18180, Abcam, UK, 1:500), ABCG1 antibody (ab52617, Abcam, UK, 1:500) and LXRα antibody (ab41902, Abcam, UK, 1:500) overnight at 4°C, and then incubated with secondary antibody anti-rabbit/mouse-HRP (Santa Cruz Biotech, Santa Cruz, CA, 1:2000) for 1 hour at 37°C Image-Pro Plus 6.

Cui Y., Liu J., Huang C, & Zhao B. (2019). Moxibustion at CV4 alleviates atherosclerotic lesions through activation of the LXRα/ABCA1 pathway in apolipoprotein-E-deficient mice. Acupuncture in Medicine, 37(4), 237-243.

+ Open protocol

+ Expand

Western Blot Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total cell protein were electrophoresed in 10% SDS-PAGE gel and blotted onto a polyvinylidene difluoride membrane. After being blocked with 5% powdered skim milk for 2 h in phosphate-buffered saline containing 0.1% Tween 20 (PBST), the membranes were incubated with antibody (1∶500 diluted, Abcam, Cambridge, UK), overnight at 4°C, and then incubated with secondary antibody anti-rabbit/mouse-HRP (1∶2000 diluted, Santa Cruz Biotech, Santa Cruz, CA). GAPDH antibody (1∶200 diluted, Santa Cruz Biotech, Santa Cruz, CA) was used as a control. All bands were detected using ECL Western blotting kit (Amersham Biosciences, UK), according to the manufacturer's instruction.

Ma H., Cheng L., Hao K., Li Y., Song X., Zhou H, & Jia L. (2014). Reversal Effect of ST6GAL 1 on Multidrug Resistance in Human Leukemia by Regulating the PI3K/Akt Pathway and the Expression of P-gp and MRP1. PLoS ONE, 9(1), e85113.

+ Open protocol

+ Expand

Western Blot Analysis of Regulatory Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein lysates from cell lines were analyzed by Western blot. Relevant primary antibodies against FOXC1 (Cell Signaling Technology - #8758), NFIX (Invitrogen - #PA5-31234), POU4F1 (Santa Cruz Biotechnology – sc-8429) were used to detect target genes and GAPDH (mouse αGAPDH – Abcam – ab8245; rabbit αGAPDH – Cell Signaling Technology – 2118L) was used as a housekeeping gene. Secondary antibodies mouse anti-rabbit HRP (Santa Cruz Biotechnology – sc-2054) and goat anti-mouse HRP (Jackson ImmunoResearch – 115-035-062) enabled detection and quantifications by densitometry using Imagelab software and a GelDoc imager.

Assi S.A., Imperato M.R., Coleman D.J., Pickin A., Potluri S., Ptasinska A., Chin P.S., Blair H., Cauchy P., James S.R., Zacarias-Cabeza J., Gilding L.N., Beggs A., Clokie S., Loke J.C., Jenkin P., Uddin A., Delwel R., Richards S.J., Raghavan M., Griffiths M.J., Heidenreich O., Cockerill P.N, & Bonifer C. (2018). Subtype-specific regulatory network rewiring in acute myeloid leukemia. Nature genetics, 51(1), 151-162.

+ Open protocol

+ Expand

Western Blot Analysis of STING Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols

At 24 hours post-transfection, growth media was aspirated, cells were carefully washed with PBS, and subsequently lysed with Cell Extraction Buffer (Life Tech) with added Pierce protease and phosphatase inhibitors. The lysates were incubated on ice for 15 min followed by centrifugation for 5 min at ≥8000 rcf and 4 °C. LDS Sample Buffer was added to cell lysates at a final concentration of 1× followed by incubation at 100 °C for 5 min. The lysates were then subjected to SDS-PAGE on NuPAGE™ 4%–12% Bis-Tris precast Protein Gels (Invitrogen). Proteins were then transferred from SDS-PAGE gel to polyvinylidene fluoride membrane and detected using the following primary antibodies: rabbit anti-STING (1:2000; Cell Signaling), rabbit anti-TBK1 (1:1000; Cell Signaling), and rabbit anti-IRF3 (1:1000; Cell Signaling), rabbit anti-cGAS (1:2000; Millipore), mouse anti-HA.11 (1:3000, Biolegend), monoclonal SV40 T-antigen antibody (1:1000 dilution; Pab416, Santa Cruz Biotech). Secondary antibodies used: anti-mouse IgGκ HRP (1:3000, 1:5000 for anti-HA.11; Santa Cruz Biotech) and mouse anti-rabbit HRP (1:3000; Santa Cruz Biotech). SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific) was used to visualize proteins following secondary antibody incubation.

Reus J.B., Trivino-Soto G.S., Wu L.I., Kokott K, & Lim E.S. (2020). SV40 Large T Antigen Is Not Responsible for the Loss of STING in 293T Cells but Can Inhibit cGAS-STING Interferon Induction. Viruses, 12(2), 137.

+ Open protocol

+ Expand

Immunoblot Analysis of Cell Signaling

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunoblots were performed as described previously^{38 (link),77 (link)}. Briefly, cells were harvested and pellets were lysed and prepared via boiling for 15 min in 1X loading dye (50 mM Tris [pH 6.8], 100 mM DTT, 2% SDS, 0.1% bromophenol blue, 10% glycerol). Lysates were separated by SDS-PAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto 0.45 µm nitrocellulose membrane. Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Aiolos (39293, Active Motif, 1:20,000), Bcl-6 (clone K112, BD Biosciences, 1:500), pSTAT5_(Y694/9) (clone 47 BD Biosciences, 1:5000), STAT5 (clone (D206Y, Cell Signaling, 1:5000), β-Actin (GenScript, 1:15,000), Eomes (Abcam, 1:5,000) goat anti-mouse:HRP (Jackson Immunoresearch, 1:5,000-1:30,000), mouse anti-rabbit:HRP (Santa Cruz, 1:5000-1:20,000).

Read K.A., Jones D.M., Pokhrel S., Hales E.D., Varkey A., Tuazon J.A., Eisele C.D., Abdouni O., Saadey A., Leonard M.R., Warren R.T., Powell M.D., Boss J.M., Hemann E.A., Yount J.S., Xin G., Ghoneim H.E., Lio C.W., Freud A.G., Collins P.L, & Oestreich K.J. (2023). Aiolos represses CD4+ T cell cytotoxic programming via reciprocal regulation of TFH transcription factors and IL-2 sensitivity. Nature Communications, 14, 1652.

+ Open protocol

+ Expand

Phosphorylation Analysis of Arabidopsis Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

The kinase reactions, with and without SSPbP22, together with Arabidopsis total protein extracted in PEB protein extraction buffer, following the manufacturer's recommendations (Agrisera, Vännäs, Sweden), were immobilized on 0.2‐μm Immun‐Blot^® PVDF Membrane (Bio‐Rad) using a 96‐well Bio‐Dot^® (Bio‐Rad). Phosphorylated proteins were visualized using 1:1,000 polyclonal anti‐Phospho‐(Ser/Thr) antibody (Abcam), followed by mouse anti‐rabbit‐HRP (Santa Cruz Biotechnology). Blocking was performed with 5% skim milk (BD Difco, Mississauga, ON, Canada). The chemiluminescent signal from the horseradish peroxidase (HPR) was observed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific) through a ChemiDoc™ Imaging System (Bio‐Rad).

Pérez‐López E., Hossain M.M., Tu J., Waldner M., Todd C.D., Kusalik A.J., Wei Y, & Bonham‐Smith P.C. (2020). Transcriptome Analysis Identifies Plasmodiophora brassicae Secondary Infection Effector Candidates. The Journal of Eukaryotic Microbiology, 67(3), 337-351.

+ Open protocol

+ Expand

Western Blot Analysis of Regulatory Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!