H. pylori CagA+ strains, “J166” a clinical isolate of human-derived H. pylori, and “7.13” a rodent adapted strain derived from B128 H. pylori23 (link)–25 (link), were used in the in vitro studies. For the in vivo study, we used the wild-type rodent-adapted cag+ H. pylori strain PMSS1, a clinical isolate of a duodenal ulcer patient and the parental strain of the mouse-derivative Sydney strain 1 (SS1)26 (link). All H. pylori cultures were performed on brucella agar (BBL/Becton Dickinson, Sparks, MD) supplemented with 5% heat-inactivated newborn calf serum (Invitrogen) and ABPNV (amphotericin B, 20 mg/liter; bacitracin, 200 mg/liter; polymyxin B, 3.3 mg/liter; nalidixic acid, 10.7 mg/liter; vancomycin, 100 mg/liter) antibiotics (Sigma-Aldrich, St. Louis, MO). H. pylori liquid cultures for mouse inoculation were grown in brucella broth with 5% NCS and antibiotic supplementation for approximately 24 h (optical density at 600 nm 0.35 to 0.45), pelleted by centrifugation, and suspended in brucella broth.
Abpnv
Manufactured by Thermo Fisher Scientific
The ABPNV is a laboratory equipment product. It serves a core functional purpose without any additional interpretation or extrapolation on its intended use.
Automatically generated - may contain errors
Lab products found in correlation
Bacitracin, by Merck Group (2 mentions)
Vancomycin, by Merck Group (2 mentions)
Nalidixic acid, by Merck Group (2 mentions)
Polymyxin b, by Merck Group (2 mentions)
Heat inactivated newborn calf serum, by Thermo Fisher Scientific (1 mentions)
Newborn calf serum, by Thermo Fisher Scientific (1 mentions)
Brucella broth, by BD (1 mentions)
2 protocols using abpnv
1
H. pylori Strains for In Vitro and In Vivo Studies
2
H. pylori Strains for In Vitro and In Vivo Studies
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For the in vitro studies, we used two CagA+ H. pylori carcinogenic strains, “J166” a clinical isolate of human-derived H. pylori, and “7.13” a rodent adapted strain derived from B128 H. pylori, which have been described previously [24 (link), 44 (link)–47 (link)]. In the in vivo study, we used the wild-type rodent-adapted cagA+ H. pylori strain (PMSS1), a clinical isolate from a duodenal ulcer patient and the parental strain of the mouse-derivative Sydney strain 1 (SS1) [48 (link)]. All H. pylori plate cultures were performed on Brucella agar (BBL/Becton Dickinson, Sparks, MD) supplemented with 5% heat-inactivated newborn calf serum (Invitrogen) and ABPNV (amphotericin B, 20 mg/liter; bacitracin, 200 mg/liter; polymyxin B, 3.3 mg/liter; nalidixic acid, 10.7 mg/liter; vancomycin, 100 mg/liter) antibiotics (all from Sigma-Aldrich, Milwaukee, WI). H. pylori MPSS1 strain liquid cultures for mouse inoculation were grown in Brucella broth (BD Biosciences) with 5% NCS and antibiotic supplementation for approximately 24 h, pelleted by centrifugation, and suspended in Brucella broth.
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