Primary antibodies were from the following sources: anti- poly (ADP-ribose) polymerase (PARP) (9532S), anti−cleaved caspase-3 (9664S), anti−cyclophilin B (43603S), anti-Phospho-RIP1 (Ser166) (31122S), anti-Phospho-RIP3 (Thr231/Ser232) (57220S), anti-Phospho-MLKL (Ser345) (37333S), anti-Rb (9313S) and anti-Phospho-Rb (8180S) from Cell Signaling Technology (Danvers, MA);anti-mTIM-1 (Kim-1) (AF1817) from R&D system (Minneapolis, MN); anti-PFKFB3 (ab181861 and ab218121) from Abcam (Waltham, MA); anti-CDK4 (A0366) from Abclonal (Woburn, MA) and (sc-23896) from Santa Cruz Biotechnology (Dallas, TX); anti−β-actin (A5441) Sigma-Aldrich (St. Louis, MO). Cisplatin was purchased from Sigma-Aldrich and West-Ward Pharmaceuticals (Berkeley Heights, NJ, USA). 1-(4pyridinyl)-3-(2-quinolinyl)-2-propen-1-one (PFK15, an inhibitor of PFKFB3) was purchased from Selleck Chemicals (Houston, TX). Ribociclib (an inhibitor of CDK4/6) was purchased from Chemietek (Indianapolis, IN).
1 4pyridinyl 3 2 quinolinyl 2 propen 1 one pfk15
Manufactured by Selleck Chemicals
1-(4pyridinyl)-3-(2-quinolinyl)-2-propen-1-one (PFK15) is a chemical compound used in laboratory research applications. It has the molecular formula C₁₇H₁₂N₂O. No further details on its core function or intended use can be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin a5441, by Merck Group (1 mentions)
Ribociclib, by Chemietek (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Phosphor rb, by Cell Signaling Technology (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Cyclin d1, by Cell Signaling Technology (1 mentions)
Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (1 mentions)
Epithelial growth factor (egf), by Thermo Fisher Scientific (1 mentions)
Pfkfb3, by Cell Signaling Technology (1 mentions)
Matrigeltm matrix, by BD (1 mentions)
Transwell boyden chamber system, by Corning (1 mentions)
6 well ultra low adherence plate, by Corning (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Ribonuclease a, by Merck Group (1 mentions)
Gelatin, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Bcl 2, by Abcam (1 mentions)
2 protocols using 1 4pyridinyl 3 2 quinolinyl 2 propen 1 one pfk15
1
Inhibition of PFKFB3 and CDK4/6 in Cancer
2
Breast Cancer Cell Culture Protocol
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Dulbecco’s modified Eagle’s medium (DMEM), DMEM/F12, fetal bovine serum (FBS), penicillin and streptomycin were obtained from GIBCO (Carlsbad, CA). 1-(4-pyridinyl)-3-(2-quinolinyl)-2-propen-1-one (PFK15; Selleck, Houston, TX), dimethylsulfoxide (DMSO; Sigma-Aldrich, St Louis, MO), Gelatin, propidium iodide (PI) and Ribonuclease A (Sigma-Aldrich, St Louis, MO), Mounting Medium with DAPI (Zhongshan, Beijing, China) were purchased. MatrigelTM matrix (BD Biosciences, San Jose, CA), transwell Boyden chamber system and 6-well Ultralow Adherence plates (Corning life Sciences, Wilkes Barre, PA), 0.5% Triton X-100 (MP Biomedical, Solon, OH) were also used. Epithelial growth factor (EGF) and basic fibroblast growth factor (bFGF) were purchased from Peprotech (Rocky Hill, NJ). Antibodies including PFKFB3, phosphor-Rb, cyclinD1, cleaved-caspase3 (Cell Signaling, Danvers, MA), Bcl2 (Abcam, Cambridge, UK) and β-Actin (Santa Cruz, CA) were purchased. All other chemicals were classified as analytical grade reagents.
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