Tissues were cut into 2-3 mm slices and fixed in IHC zinc fixative solution(Beckstead, 1994 (link)) (BD Biosciences) for 24 hours at room temperature (RT). After rinsing with tap water for 45 min, tissues were dehydrated at RT for 45 min each with 70% ethanol, 95% ethanol 100% ethanol and xylene, respectively. Tissues were infiltrated in paraffin at 58°C for 45 min using a Sakura Tissue-Tek®IV Embedding center (Sakura, Mars, PA). Tissue sections were prepared by cutting at 4 μm and floated out on a water bath at 43°C and collected on coated glass slides (SuperFrost/Plus; Fisher Scientific, Pittsburgh, PA). The slides were dried at RT for overnight.
Ihc zinc fixative solution
Manufactured by BD
IHC zinc fixative solution is a laboratory reagent used for the preservation and preparation of biological samples for immunohistochemical (IHC) analysis. It functions as a fixative, stabilizing the cellular structure and proteins within the sample to prevent degradation.
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Lab products found in correlation
2 protocols using ihc zinc fixative solution
1
Tissue Preparation for Immunohistochemistry
2
Immunohistochemical Analysis of Adhesion Lesions
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Immunohistochemistry was performed to test whether fibrinogen and collagen type I were present in the adhesion lesions and exudates. Tissue specimens were fixed for 24 h in IHC Zinc fixative solution (BD Biosciences) and embedded in paraffin wax before sectioning. Sections of 4 µm thickness for each specimen were prepared on peeling prevention coated slide glass (Matsunami Glass Ind.) and stained with hematoxylin and eosin (H&E). For immunohistochemistry, the sections were preincubated with serum-free protein block (Agilent Technologies) for 30 min at room temperature and primary antibodies for fibrinogen (ab92572, Abcam) and collagen type I (F56, Kyowa Pharma Chemical Co. Ltd.) diluted in PBS were added to each slide overnight at 4 °C. Secondary antibody staining was performed using the Histofine Simple Stain MAX-PO (Nichirei) according to the manufacturer’s instructions. The staining was visualized under a Nikon Eclipse TS-100 microscope (Nikon, Tokyo, Japan).
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