Cd24 pe

Manufactured by Miltenyi Biotec

Sourced in Germany

The CD24-PE is a fluorochrome-conjugated antibody that binds to the CD24 antigen. CD24 is a cell surface glycoprotein expressed on various cell types. The PE (Phycoerythrin) fluorochrome provides a bright signal for the detection of CD24-positive cells using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Facscanto 2 flow cytometer, by BD (2 mentions) Cd271 pe, by Miltenyi Biotec (2 mentions) Cd26 pe, by Miltenyi Biotec (2 mentions) Lsr 2 flow cytometer, by BD (1 mentions) Facsdiva software, by BD (1 mentions) Cd44 fitc, by Thermo Fisher Scientific (1 mentions) Epcam fitc antibody, by Miltenyi Biotec (1 mentions) Cd44 apc, by Miltenyi Biotec (1 mentions) Cd44 pe, by Miltenyi Biotec (1 mentions) Cd133 2 pe, by Miltenyi Biotec (1 mentions) Facscanto 2, by BD (1 mentions) Epcam pe, by Miltenyi Biotec (1 mentions) C met apc, by R&D Systems (1 mentions)

Spelling variants of this product

PE-conjugated anti-CD24 (3 citations) Anti-CD24-PE (2 citations) PE)-conjugated anti-CD24 antibody (2 citations)

4 protocols using cd24 pe

Quantifying Breast Cancer Stem Cells

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For flow cytometry studies, MCF-7 aggregates were released from the core-shell microcapsules by treating them with 75 mM sodium citrate (~5 min to dissolve alginate) and then 1000 units/ml type I collagenase (30 min at 37 °C to remove the collagen ECM). The samples were then washed with PBS and centrifuged. The dissociated MCF-7 cells were washed with 1× PBS and stained with CD44-FITC (Invitrogen, Carlsbad, CA, USA) and CD24-PE (Miltenyi Biotec Ltd., Cambridge, MA, USA) antibodies according to the manufacturer’s instructions. Lastly, the stained samples were analyzed using a BD LSR-II Flow Cytometer together with BD FACS Diva software (Franklin Lakes, NJ, USA).

Agarwal P., Wang H., Sun M., Xu J., Zhao S., Liu Z., Gooch K.J., Zhao Y., Lu X, & He X. (2017). Microfluidics Enabled Bottom-Up Engineering of 3D Vascularized Tumor for Drug Discovery. ACS nano, 11(7), 6691-6702.

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Identifying Breast Cancer Stem Cells

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Sk-Br-3 cells were cultivated in standard CM or pr.CM for 5 days. CD24-PE, CD44-APC and EpCAM-FITC antibody (Miltenyi Biotec GmbH, Germany) were used at a 1:50 dilution and incubated for 15 min with 250.000 tumor cells per sample. Triple staining was used for analysis of CD24⁻/CD44⁺/EpCAM⁺ population on BD FACSCanto™ II Flow cytometer (Becton Dickinson, USA).

Skolekova S., Matuskova M., Bohac M., Toro L., Demkova L., Gursky J, & Kucerova L. (2016). Cisplatin-induced mesenchymal stromal cells-mediated mechanism contributing to decreased antitumor effect in breast cancer cells. Cell Communication and Signaling : CCS, 14, 4.

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Comprehensive Immunophenotyping of Cell Populations

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Following fluorochrome-conjugated antibodies were used for the evaluation of surface markers: CD44-PE, CD24-PE, CD26-PE, CD271-PE, CD133/2-PE (Miltenyi Biotec, Germany); CD166-PE (ALCAM; Immunotech, France); CD274 (PD-L1; Sony Biotechnology, USA); CD44v6-PE (RD Systems, USA); CD184 (CXCR4-PE; eBioscience, USA). Dead cells were excluded from the analysis based on DAPI staining. Cells were analyzed using BD FACSCanto™ II flow cytometer (Beckton Dickinson, USA) equipped with FacsDiva program. FCS Express software was used for the evaluation.

Durinikova E., Kozovska Z., Poturnajova M., Plava J., Cierna Z., Babelova A., Bohovic R., Schmidtova S., Tomas M., Kucerova L, & Matuskova M. (2018). ALDH1A3 upregulation and spontaneous metastasis formation is associated with acquired chemoresistance in colorectal cancer cells. BMC Cancer, 18, 848.

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Evaluation of CSCs-like Cell Markers

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For the evaluation of CSCs-like cell surface markers, the following fluorochrome-conjugated antibodies were used: CD24-PE, CD26-PE, CD44-PE/CD44-APC, CD133-PE/CD133-APC, CD271-PE, EpCAM-PE (Miltenyi Biotec, Bergisch Gladbach, Germany), CD166-PE (ALCAM, Immunotech, France), and cMET-APC (R&D Systems, Abingdon, UK). Dead cells were excluded from the analysis based on DAPI staining. Cells were analyzed by BD FACSCanto™ II flow cytometer (Beckton Dickinson, USA) equipped with FacsDiva program. FCS Express software was used for the evaluation.

Tyciakova S., Valova V., Svitkova B, & Matuskova M. (2021). Overexpression of TNFα induces senescence, autophagy and mitochondrial dysfunctions in melanoma cells. BMC Cancer, 21, 507.

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