Blood samples were collected from the cut tail vein of animals. Blood glucose was measured using a portable Ascencia Contour blood glucose meter (Bayer Healthcare, Newbury, Berkshire, UK). For plasma insulin and glucagon, blood was collected in chilled fluoride/heparin coated microcentrifuge tubes (Sarstedt, Numbrecht, Germany) and centrifuged using a Beckman micro-centrifuge (Beckman Instruments, Galway, Ireland) for 10 min at 12,000 rpm. Plasma was removed and stored at −20 °C, until required for analysis. For hormone content, snap frozen pancreatic tissues were homogenised in acid/ethanol (75% (v/v) ethanol, distilled water and 1.5% (v/v) 12 M HCl) and protein extracted in a pH neutral TRIS buffer, with protein content determined using Bradford reagent (Sigma-Aldrich). Plasma and pancreatic insulin content were determined by an in-house insulin RIA [24 (link)], whilst plasma and pancreatic glucagon content were assessed by a commercially available ELISA kit (glucagon chemiluminescent assay, EZGLU-30K, Millipore) following the manufacturer’s guidelines.
Beckman microcentrifuge
Manufactured by Beckman Coulter
Sourced in Germany, Ireland
The Beckman microcentrifuge is a compact, high-performance centrifuge designed for laboratory applications. It provides reliable and efficient separation of samples in microtubes or microplates. The microcentrifuge offers precise speed control and rapid acceleration and deceleration to ensure consistent and reproducible results.
Automatically generated - may contain errors
Lab products found in correlation
Ezglu 30k, by Merck Group (8 mentions)
Ascencia contour, by Bayer (5 mentions)
Fluoride heparin coated micro centrifuge tubes, by Sarstedt (5 mentions)
Fluoride heparin glucose micro centrifuge tubes, by Sarstedt (5 mentions)
Ascencia contour blood glucose meter, by Bayer (3 mentions)
Bradford reagent, by Merck Group (2 mentions)
Ascencia meter, by Bayer (2 mentions)
Fluoride heparin coated tubes, by Sarstedt (2 mentions)
Eglp 35k, by Merck Group (1 mentions)
Gl 32k, by Merck Group (1 mentions)
Beckman glucose analyser 2, by Beckman Coulter (1 mentions)
Fluoride heparin microcentrifuge tubes, by Sarstedt (1 mentions)
14 protocols using beckman microcentrifuge
1
Plasma and Pancreatic Hormone Quantification
2
Measuring Blood Glucose in Mice
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Blood samples were collected from the cut tip on the tail vein of conscious mice into chilled fluoride/heparin glucose micro-centrifuge tubes (Sarstedt, Numbrecht, Germany) at the time points indicated in the Figs Blood glucose was measured directly using a hand-held Ascencia Contour blood glucose meter (Bayer Healthcare, Newbury, Berkshire, UK). Blood samples were centrifuged using a Beckman microcentrifuge (Beckman Instruments, Galway, Ireland) for 1 min at 13,000 x g and stored at -20°C. Plasma and pancreatic insulin were assayed by a modified dextran-coated charcoal radioimmunoassay [19 (link)].
3
Blood Glucose and Hormone Quantification
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Blood samples were collected from the tail vein of animals into ice-chilled heparin-coated microcentrifuge tubes. Blood glucose was measured using a portable Ascencia meter (Bayer Healthcare, Newbury, Berkshire, UK). For plasma insulin and glucagon, blood was collected in chilled fluoride/heparin-coated tubes (Sarstedt, Numbrecht, Germany) and centrifuged using a Beckman microcentrifuge (Beckman Instruments, Galway, Ireland) for 10 min at 12,000 rpm. Plasma was then stored at – 20 °C. For hormone determination from tissues, samples underwent acid–ethanol extraction (HCl: 1.5% v/v, ethanol: 75% v/v, H2O:23.5% v/v). Insulin concentrations were subsequently assessed by an in-house radioimmunoassay [34 (link)]. Plasma glucagon and pancreatic glucagon content were measured using glucagon ELISA (EZGLU-30 K, Merck Millipore), or RIA kit (250-tubes GL-32 K, Millipore, USA), respectively.
4
Plasma Insulin and Glucagon Measurement
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Blood samples were obtained from conscious mice via the cut tip on the tail vein and blood glucose immediately measured using an Ascencia Contour blood glucose meter (Bayer Healthcare Newbury, UK). Blood was collected in chilled heparin/fluoride coated micro-centrifuge tubes (Sarstedt, Numbrecht, Germany) and centrifuged for 15 minutes at 12,000 rpm using a Beckman microcentrifuge (Beckman Instruments, Galway, Ireland) to separate plasma. Insulin and glucagon were then measured by radioimmunoassay (32 (link)) or commercially available ELISA (EZGLU-30K, Merck Millipore, Burlington, Massachusetts), respectively.
5
Analytical Techniques for Metabolic Hormones
6
Blood Glucose and Hormone Quantification
7
Measurement of Plasma and Pancreatic Hormones
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Blood samples were collected from the cut tail vein of animals. Blood glucose was measured using a portable Ascencia Contour blood glucose meter (Bayer Healthcare, Newbury, Berkshire, UK). For plasma insulin and glucagon, blood was collected in chilled fluoride/heparin coated microcentrifuge tubes (Sarstedt, Numbrecht, Germany) and centrifuged using a Beckman micro-centrifuge (Beckman Instruments, Galway, Ireland) for 10 min at 12,000 rpm. Plasma was extracted and stored at −20°C, until required for analysis. For hormone content, snap frozen pancreatic tissues were homogenized in acid/ethanol (75% (v/v) ethanol, distilled water and 1.5% (v/v) 12 M HCl) and protein extracted in a pH neutral TRIS buffer. Protein content was determined using Bradford reagent (Sigma-Aldrich). Plasma and pancreatic insulin content were determined by an in-house insulin RIA (28 (link)), while plasma and pancreatic glucagon content were assessed by a commercially available ELISA kit (Glucagon chemiluminescent assay, EZGLU-30K, Millipore) following the manufacturer’s guidelines.
8
Blood Glucose and Insulin Analysis in Mice
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An incision to the tail vain of conscious mice was used to obtain blood samples
for biochemical analysis. Blood glucose was measured directly by an Ascencia
Contour glucose metre (Bayer, Newbury, UK). For plasma insulin analyses, blood
samples were collected into chilled fluoride/heparin glucose microcentrifuge
tubes (Sarstedt, Numbrecht, Germany) and immediately centrifuged using a Beckman
microcentrifuge (Beckman Instruments, Galway, Ireland) for 1 minute at 13 000 ×
g and stored at −20°C prior to insulin RIA.37 (link)
for biochemical analysis. Blood glucose was measured directly by an Ascencia
Contour glucose metre (Bayer, Newbury, UK). For plasma insulin analyses, blood
samples were collected into chilled fluoride/heparin glucose microcentrifuge
tubes (Sarstedt, Numbrecht, Germany) and immediately centrifuged using a Beckman
microcentrifuge (Beckman Instruments, Galway, Ireland) for 1 minute at 13 000 ×
g and stored at −20°C prior to insulin RIA.37 (link)
9
Measuring Blood Glucose, Insulin, and Glucagon
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Blood samples were collected from the cut tip on the tail vein, of conscious mice, at times indicated in Figures. Blood glucose was measured immediately using a hand-held Ascencia Contour blood glucose meter (Bayer Healthcare, Newbury, Berkshire, UK). For plasma insulin and glucagon, blood was collected in chilled fluoride/heparin coated micro-centrifuge tubes (Sarstedt, Numbrecht, Germany) and centrifuged using a Beckman micro-centrifuge (Beckman Instruments, Galway, Ireland) for 10 minutes at 12,000 rpm. Plasma was separated and stored at -20 o C, until determination of plasma insulin by radioimmunoassay [17] or glucagon by a commercially available ELISA kits (EZGLU-30K, Merck Millipore).
10
Plasma Glucose and Insulin Measurement in Mice
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From the cut tip of the tail vein of conscious mice, blood samples were collected into fluoride/ heparin microcentrifuge tubes (Sarstedt, Numbrecht, Germany) at the times indicated in the Figures. Samples were immediately centrifuged (Beckman microcentrifuge; Beckman Instruments, High Wycombe, UK) for 30 s at 13,000 g and resulting plasma was stored at -20°C until analysis. Plasma and pancreatic insulin levels were determined by an insulin radioimmunoassay as described previously [18] . Plasma glucose was assayed by an automated glucose oxidase procedure using a Beckman Glucose Analyser II (Beckman Instruments, Galway, Ireland).
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