Plasma levels of HMGB1, CytC, TNF, P-sel, and GDF15 were determined using commercial kits as follows: HMGB-1 ELISA kit (Elabscience, Houston, TX, USA), CytC ELISA kit (Elabscience, Houston, TX, USA), Human TNFα High Sensitivity ELISA kit (Invitrogen, ThermoFisher Scientific, Wien, Austria), Human sP-selectin ELISA kit (Invitrogen, ThermoFisher Scientific, Wien, Austria), and Human GDF-15 ELISA kit (Thermo Scientific, Waltham, MA, USA), according to the manufacturer’s instructions. The absorbance signals of the ELISA were determined on a spectrophotometer (µQuant, Biotek, Winooski, VT, USA).
Hmgb1 elisa kit
Manufactured by Elabscience
Sourced in United States
The HMGB1 Elisa kit is a quantitative sandwich enzyme immunoassay designed for the measurement of HMGB1 levels in various biological samples.
Automatically generated - may contain errors
4 protocols using hmgb1 elisa kit
1
Quantifying Plasma Biomarkers in Research
2
Quantification of Inflammatory Markers
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Human serum HMGB1 were quantified by the HMGB1 ELISA Kit (Elabscience, Wuhan, China). After anesthesia, mouse blood samples were obtained through the retro-orbital sinus. Serums were collected by centrifugation at 2,500 rpm at 4°C for 15 min for ELISA test. IL-6 (Bio-swamp, Wuhan, China), TNF-α (Bio-swamp, Wuhan, China), HMGB1 (Elabscience, Wuhan, China) were quantitatively detected according to the instructions.
3
HMGB1 Elisa Quantification in Treated Cells
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The HMGB1 Elisa kit was purchased from Elabscience Biotechnology Co, Ltd. Both Jurkat and HL-60 cells are treated with ADM (0, 0.05, 0.1, 0.2, 0.4, 0.8 µM) for 24 h, then the supernate was collected. We diluted the supernate at 1:2 and added into the wells. The supernate and the reaction mixture were incubated in a 37 °C incubator for 30 min. Then the absorbance at 450 nm was measured in a microplate reader and we calculated each wells’ content of HMGB1 according to the standard curve.
4
HMGB1 Secretion in Tumor Cell Lines
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The HMGB1 Elisa kit was purchased from Elabscience Biotechnology Co,Ltd. Both Jurkat and HL-60 cells are treated with ADM(0, 0.05, 0.1, 0.2, 0.4, 0.8µM)for 24h, then the supernate was collected. We diluted the supernate at 1:2 and added into the wells. The supernate and the reaction mixture were incubated in a 37˚C incubator for 30 minutes. Then the absorbance at 450 nm was measured in a microplate reader and we calculated each wells' content of HMGB1 according to the standard curve.
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