Immunocytochemistry was performed essentially as described in.45 Images were taken with a Zeiss Axiovert confocal microscope equipped with a LSM520 Meta. Anti-Cleaved Caspase-3 (Asp175) (Cell Signaling, cat no. 9661) was used at 1:400 dilution. Cy3-conjugated monoclonal donkey anti-mouse IgG secondary antibody was used at 1:400 (Jackson ImmunoResearch, cat. no.715–165–151).
Cy3 conjugated monoclonal donkey anti mouse igg secondary antibody
Manufactured by Jackson ImmunoResearch
Cy3-conjugated monoclonal donkey anti-mouse IgG secondary antibody is a reagent used in immunoassays and immunohistochemistry applications. It binds to mouse immunoglobulin G (IgG) and is labeled with the fluorescent dye Cy3, which emits light in the red-orange spectrum when excited.
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Lab products found in correlation
2 protocols using cy3 conjugated monoclonal donkey anti mouse igg secondary antibody
1
Immunocytochemistry Procedure for Cleaved Caspase-3
2
Quantifying Mitosis in Drosophila Imaginal Discs
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Immunocytochemistry was performed essentially as described in [1 (link)] on 120hr L3 wing imaginal discs aged using 1 hour egg lay periods. Images were taken with a Leica SP8 confocal microscope. Anti-Phosphohistone H3 (Cell Signalling, cat no. 9701) was used at 1:300 dilution. Cy3-conjugated monoclonal Donkey anti-mouse IgG secondary antibody was used at 1:400 (Jackson ImmunoResearch, cat. no.715-165-151). The number of nuclei undergoing mitosis were counted using the ImageJ plugin DeadEasy MitoGlia [42 (link)]. The mitotic index was then calculated for each disc by dividing the number of cells in M phase by the area of the disc.
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