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BAF53 is a subunit of the SWI/SNF chromatin remodeling complex. It plays a role in regulating gene expression by modulating chromatin structure.

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Lab products found in correlation

E cadherin, by Cell Signaling Technology (1 mentions) Smarcc1 baf155, by Cell Signaling Technology (1 mentions) Cytokeratin 19, by Santa Cruz Biotechnology (1 mentions) Arid1a baf250a, by Cell Signaling Technology (1 mentions) Smarcb1 baf47, by Cell Signaling Technology (1 mentions) Baf60a, by Santa Cruz Biotechnology (1 mentions) Ki 67 mib 1, by Agilent Technologies (1 mentions) Vimentin, by Cell Signaling Technology (1 mentions) Nvp auy 922, by LC Laboratories (1 mentions) Arid1b, by Abcam (1 mentions) Baf57, by Fortis Life Sciences (1 mentions) Arid1a, by Merck Group (1 mentions) Arid1a, by Santa Cruz Biotechnology (1 mentions) Baf155, by Santa Cruz Biotechnology (1 mentions) Baf170, by Santa Cruz Biotechnology (1 mentions) Baf60b, by Santa Cruz Biotechnology (1 mentions) Baf47, by Santa Cruz Biotechnology (1 mentions)

2 protocols using baf53

1

Comprehensive Immunohistochemical Profiling

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Primary antibodies used for immunoblotting, co-immunofluorescence (co-IF), and immunohistochemistry: ARID1A/BAF250A (Cell Signaling Technologies, D2A8U), VIMENTIN (Cell Signaling Technologies, D21H3) for co-IF with E-CADHERIN and immunohistochemistry, E-CADHERIN (Cell Signaling Technologies, 4A2), Cytokeratin 19 (Santa Cruz Biotechnologies, A-3), KI-67 (MIB-1) (Dako), Cleaved CASPASE 3 (A175) (Cell Signaling Technologies, 5A1E), HSP90 alpha (Invitrogen, PA3–013), VIMENTIN (Cell Signaling Technologies, 5G3F10) for immunoblotting and co-IF with HSP90. ARID1B/BAF250B (Cell Signaling Technologies, E9J4T), PBRM1/BAF180 (Cell Signaling Technologies, D3F7O), BRM (Cell Signaling Technologies, D9E8B), BRG1 (Cell Signaling Technologies, A52), SMARCC1/BAF155 (Cell Signaling Technologies, D7F8S), SMARCC2/BAF170 (Cell Signaling Technologies, D8O9V), BAF60a (Santa Cruz Biotechnologies, 23), BAF53 (Santa Cruz Biotechnologies, C-7), SMARCB1/BAF47 (Cell Signaling Technologies, D8M1X). Chemical reagents: NVP-AUY-922 (LC Laboratories).
Tomihara H., Carbone F., Perelli L., Huang J.K., Soeung M., Rose J.L., Robinson F.S., Deribe Y.L., Feng N., Takeda M., Inoue A., Del Poggetto E., Deem A.K., Maitra A., Msaouel P., Tannir N.M., Draetta G.F., Viale A., Viale A., Heffernan T.P., Bristow C.A., Carugo A, & Genovese G. (2020). Loss of ARID1A promotes epithelial-mesenchymal transition and sensitizes pancreatic tumors to proteotoxic stress. Cancer research, 81(2), 332-343.
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2

Characterizing Chromatin Remodeling Factors

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ARID1A (Sigma-Aldrich #HPA005456, western blot and IHC), ARID1A (Santa Cruz #sc-32761, western blot), ARID1B (Abcam #AB57461, western blot and IHC), ARID2 (Abiocode #R2380–1, western blot), Brg1 (Santa Cruz #sc-10768, western blot), BAF170 (Santa Cruz #sc-17838, western blot), BAF155 (Santa Cruz #sc-9746, western blot), BAF60b (Santa Cruz #sc-101162, western blot), BAF57 (Bethyl #A300–810A, western blot), BAF53 (Santa Cruz #sc-137063, western blot), BAF47 (Santa Cruz #sc-166165, western blot), BAF47 (CST #91735, western blot), BAF45d (Santa Cruz #sc-101106, western blot), Ki-67 (Abcam #AB15580, IHC), HNF4A (CST #3113, IHC), CK19 (Abcam #AB15463, IHC), EpCAM (CST #14452, IHC), Flag (CST #2368, western blot), Ty1 (Diagenode #C15200054, western blot and ChIP).
Wang Z., Chen K., Jia Y., Chuang J.C., Sun X., Lin Y.H., Celen C., Li L., Huang F., Liu X., Castrillon D.H., Wang T, & Zhu H. (2020). Dual ARID1A/ARID1B loss leads to rapid carcinogenesis and disruptive redistribution of BAF complexes. Nature cancer, 1(9), 909-922.
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