Mouse embryos isolated at E16.5 were imaged using a Nikon SMZ1500 Stereo Fluorescence Microscope. For flow cytometry, isolated tissues were incubated for 30 min at 37°C in 0.25% trypsin. During this time, tissues were disassociated using progressively smaller pipet tips (1000 μl to 200 μl), followed by analysis on an LSRII or sorting on a FACSAria (BD Biosciences).
Smz1500 stereo fluorescence microscope
Manufactured by Nikon
The Nikon SMZ1500 Stereo Fluorescence Microscope is a high-performance optical instrument designed for scientific applications. It features a zoom ratio of 15:1, providing a wide range of magnification from 0.75x to 11.25x. The microscope is equipped with incident and transmitted LED illumination, enabling both fluorescent and brightfield observation. It is compatible with a range of accessories to accommodate various experimental needs.
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Lab products found in correlation
2 protocols using smz1500 stereo fluorescence microscope
1
Isolation and Flow Cytometry of E16.5 Mouse Embryos
2
E15.5 Mouse Embryo Imaging and Flow Cytometry
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Mouse embryos isolated at E15.5 were imaged using a Nikon SMZ1500 Stereo Fluorescence Microscope. For flow cytometry, tissues were isolated in the same manner as MEF derivations (described above), followed by analysis on an LSRII cytometer (BD Biosciences) and analyzed with FlowJo software (Tree Star Inc.).
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