Nci h82

Manufactured by American Type Culture Collection

Sourced in United States

The NCI-H82 is a laboratory cell line derived from human small cell lung carcinoma. It is a widely used model for research on small cell lung cancer.

Automatically generated - may contain errors

Lab products found in correlation

35 protocols using nci h82

Cell Culture Conditions for SCLC Lines

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell lines were cultured in RPMI-1640 (Millipore Sigma R0883) except for 293T cells and cell lines derived from RPR2;R26^Luc and RPR2;R26^Dll3, which were cultured in DMEM (Hyclone SH30243.01). RPMI and DMEM were supplemented with 10% bovine growth serum (Hyclone SH3054103HI) and penicillin-streptomycin-glutamine (Gibco 10378016). Cells were grown at 37°C in standard cell culture incubators. All cells were routinely tested (MycoAlert Detection Kit, Lonza) and confirmed to be free of mycoplasma contamination.
NCI-H524, NCI-H82, and NCI-H1694 cells were purchased from ATCC. Murine KP11B6 (a negative control for tdTomato expression in Figure S3B) and HES1^GFP/+ SCLC cell lines were generated in the lab and have been described.⁶³ (link)

Kim J.W., Ko J.H, & Sage J. (2022). DLL3 regulates Notch signaling in small cell lung cancer. iScience, 25(12), 105603.

+ Open protocol

+ Expand

Culturing Small Cell Carcinoma and Adenocarcinoma Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human small cell carcinoma cell lines (NCI-H69; ATCC#HTB-119 and NCI-H82; ATCC#HTB-171) and human adenocarcinoma cell lines (PC9; RIKEN BioResource Center#RCB4455) were originally purchased from ATCC or RIKEN BioResource Center and stocked at our institution. The CAFs were obtained from surgically resected small cell carcinoma specimens (Supplementary Table 1), and were cultured according to a previously described method. [28 (link), 29 (link)] NCI-H69 and NCI-H82 were cultured in RPMI1640 (SIGMA-Aldrich, MO) containing 10% fetal bovine serum (FBS; Nichirei Bioscience, Japan) and 1% penicillin and streptomycin (SIGMA-Aldrich). The CAFs were cultured in MEM alpha (Life Technologies Corporation [Gibco], CA) supplemented with 10% FBS and 1% penicillin and streptomycin.

Takahashi A., Ishii G., Neri S., Yoshida T., Hashimoto H., Suzuki S., Umemura S., Matsumoto S., Yoh K., Niho S., Goto K., Ohmatsu H., Nagai K., Gemma A., Ohe Y, & Ochiai A. (2015). Podoplanin-expressing cancer-associated fibroblasts inhibit small cell lung cancer growth. Oncotarget, 6(11), 9531-9541.

+ Open protocol

+ Expand

SCLC Cell Line Culture Conditions

Check if the same lab product or an alternative is used in the 5 most similar protocols

Four SCLC cell lines, NCI-H82, NCI-H146, NCI-446, and NCI-H196 (ATCC, Manassas, VA) were cultured in RPMI (1:1) containing heat-inactivated fetal calf serum (FCS) (10%), L-glutamine (2mM), penicillin (100U/ml), and streptomycin (100μg/ml) (Lonza, Verviers, Belgium) at 37°C, 5% CO₂. Tetracycline-free FCS (PAN-Biotech GmbH, Aidenbach, Germany) was used for FRNK transduction experiments. HEK 293FT (ATCC) cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with heat-inactivated FCS (10%), L-glutamine (2mM), penicillin (100U/ml), streptomycin (100μg/ml) (Lonza), and neomycin (500μg/ml) (Sigma, St. Louis, MO) at 37°C, 5% CO2. The experiments were carried out on cells whose passage was between 10 and 25. For the detection of Mycoplasma in cell culture, the MycoAlert Mycoplasma Detection Kit (Lonza) was used.

Nana F.A., Lecocq M., Ladjemi M.Z., Detry B., Dupasquier S., Feron O., Massion P.P., Sibille Y., Pilette C, & Ocak S. (2018). Therapeutic potential of Focal Adhesion Kinase inhibition in small cell lung cancer. Molecular cancer therapeutics, 18(1), 17-27.

+ Open protocol

+ Expand

Cell Culture Conditions for SCLC Lines

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Except for 293T cells that were grown in DMEM, all cell lines were grown in RPMI-1640 media supplemented with 10% bovine growth serum (BGS) (Fisher Scientific) and penicillin-streptomycin-glutamine (Gibco). Murine KP1, KP2 and KP3 and human NJH29 SCLC cell lines were generated in the lab and have been described^{8 (link),33 (link),43 (link)}. GFP^neg and GFP^high cell lines were isolated by FACS from individual mice. Human NCI-H82 and NCI-H889 cells were purchased from ATCC and authenticated by STR analysis. All cell lines tested negative for mycoplasma.

Lim J.S., Ibaseta A., Fischer M.M., Cancilla B., O’Young G., Cristea S., Luca V.C., Yang D., Jahchan N.S., Hamard C., Antoine M., Wislez M., Kong C., Cain J., Liu Y.W., Kapoun A.M., Garcia K.C., Hoey T., Murriel C.L, & Sage J. (2017). Intratumoral heterogeneity generated by Notch signaling promotes small cell lung cancer. Nature, 545(7654), 360-364.

+ Open protocol

+ Expand

Cell Line Characterization and Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell lines were cultured in RPMI or HITES supplemented with 10% FBS and 1% pen/strep. GLC1, GLC8, NCI-H1092, NCI-H2141 and SBC4 were kindly provided by M. Sos (Cologne, Germany). NCI-H82, NCI-H524, NCI-H446, NCI-H889 and NCI-H69 were obtained from ATCC. NCI-H1963 was kindly provided by R. Govindan (St. Louis, MO, USA) and H1048 and DMS53 were kindly provided by D. MacPherson (Seattle, WA, USA). Cell lines were tested for Mycoplasma contamination using e-Myco PCR detection kit (Bulldog Bio: 25233) in March, 2019. GLC1, GLC8, H69, H82, H446, H524, H1092, H2141 and SBC4 were authenticated by short tandem repeat (STR) profiling in June, 2017. DMS53, H1048, H889 and H1963 were validated by STR profiling in February, 2018. Multiple vials of cell lines were cryopreserved upon acquisition and the cells were passaged for no more than 6 months in culture.

Chalishazar M.D., Wait S.J., Huang F., Ireland A.S., Mukhopadhyay A., Lee Y., Schuman S.S., Guthrie M.R., Berrett K.C., Vahrenkamp J., Hu Z., Kudla M., Modzelewska K., Wang G., Ingolia N.T., Gertz J., Lum D.H., Cosulich S.C., Bomalaski J., DeBerardinis R.J, & Oliver T.G. (2019). MYC-driven small cell lung cancer is metabolically distinct and vulnerable to arginine depletion. Clinical cancer research : an official journal of the American Association for Cancer Research, 25(16), 5107-5121.

+ Open protocol

+ Expand

SCLC Cell Lines and Assays

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

KP1 (Rb1/Trp53 mutant, DKO), and KP11 (Rb1/Rbl2/Trp53 mutant, TKO) mouse SCLC cells were generated from single tumors (Park et al., 2011 (link)). Allografts tested in the multiplexed inhibitor beads inhibitors assays were derived from single tumors. NJH29 cells were previously generated in our laboratory from a PDX model (Jahchan et al., 2013 (link)), and A549, NCI-H69, NCI-H82, NCI-H187, and NCI-H446 were purchased from ATCC. Cells were grown in RPM1-1640 media supplemented with 10% bovine growth serum (BGS, Fisher Scientific) and penicillin-streptomycin–glutamine (PSQ, Gibco). Cells were grown at 37C in standard cell culture incubators. All cell lines are routinely tested (Lonza) and confirmed to be free of mycoplasma contamination.

Coles G.L., Cristea S., Webber J.T., Levin R.S., Moss S.M., He A., Sangodkar J., Hwang Y.C., Arand J., Drainas A.P., Mooney N.A., Demeter J., Spradlin J.N., Mauch B., Le V., Shue Y.T., Ko J.H., Lee M.C., Kong C., Nomura D.K., Ohlmeyer M., Swaney D.L., Jackson P.K., Narla G., Gordan J.D., Shokat K, & Sage J. (2020). UNBIASED PROTEOMIC PROFILING UNCOVERS A TARGETABLE GNAS/PKA/PP2A AXIS IN SMALL CELL LUNG CANCER STEM CELLS. Cancer cell, 38(1), 129-143.e7.

+ Open protocol

+ Expand

Cultivation of diverse cancer cell lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

G-401, NCI-H82, SW48, MDA-MB-453 were bought from ATCC, and WSU-DLCL2 was bought from Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), SF126 cell was bought from JCRB Cell Bank. 786-O, NCI-H82, WSU-DLCL2, G-401 were cultured in 1640 medium adding 10% FBS, 1% Pen/Strep. SF-126, SW48, and HEK293T were cultured in DMEM medium adding 10% FBS, 1% Pen/Strep. CFPAC-1 was cultured in IMDM medium adding 10% FBS, 1% Pen/Strep. MDA-MB-453 was cultured in L-15 medium adding 10% FBS, 1% Pen/Strep. MDA-MB-453 was maintained in 100% air without CO₂ at 37 °C and other cells were in 5% CO₂ at 37 °C.

Liu Y., Chen Y., Wang F., Lin J., Tan X., Chen C., Wu L.L., Zhang X., Wang Y., Shi Y., Yan X, & Zhao K. (2023). Caveolin-1 promotes glioma progression and maintains its mitochondrial inhibition resistance. Discover. Oncology, 14, 161.

+ Open protocol

+ Expand

Murine Small Cell Lung Cancer Cell Establishment

Check if the same lab product or an alternative is used in the 5 most similar protocols

Murine F1339 small cell lung cancer cells were provided by Dr David MacPherson, and were produced by tracheal infection of Rb^lox/p53^lox mice with Adeno-CMV-Cre. The F1339 cells are not in the database of commonly misidentified cell lines (ICLAC and NCBI Biosample). All cell lines regularly tested negative for Mycoplasma contamination. NCI-H82 and NCI-H146 cell lines were purchased from ATCC and were authenticated by LabCorp by STR profiling at the time-of-experimentation. Cells were maintained in RPMI1640 supplemented with 10% fetal bovine serum (FBS, Gibco), 100 units/ml penicillin, 100 μg/mL streptomycin (Gibco), and cultured at 37°C in 5% CO₂.

Montgomery M.K., Xu S, & Fire A. (1998). RNA as a target of double-stranded RNA-mediated genetic interference in Caenorhabditis elegans. Proceedings of the National Academy of Sciences of the United States of America, 95(26), 15502-15507.

+ Open protocol

+ Expand

Transient Notch Overexpression in SCLC

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse (KP1) and human (NJH29, NCI-H82 and NCI-H187) SCLC cell lines were grown in RPMI-1640 media supplemented with 10% bovine growth serum (BGS) (Fisher Scientific) and penicillin-streptomycin-glutamine (Gibco), as described before⁵⁰. KP1, NJH29 were generated at Stanford. NCI-H82 and NCI-H187 were purchased from ATCC. These cells grow as suspension spheres or aggregates in culture. All cell lines were maintained at 37 °C in a humidified chamber with 5% CO₂. All cell lines tested negative for mycoplasma infection. For transient expression of Notch ICD, cells were trypsinized and transfected with either MigR1-IRES-GFP (Ctrl) or MigR1-Notch1-ICD-IRES-GFP (NICD) using Lipofectamine 2000 (Life Technologies). The plasmids were gifts from W.S. Pear (University of Pennsylvania, Philadelphia). Then 48 h after transfection, cells were trypsinized and resuspended in phosphate-buffered saline (PBS) containing 10% BGS and 1 µg ml⁻¹ 7-aminoactinomycin D (Life Technologies) that labels dead cells. Live GFP⁺ cells were then sorted for subsequent experiments using a BD FACSAria fluorescence-activated cell sorting (FACS) machine.

George J., Lim J.S., Jang S.J., Cun Y., Ozretić L., Kong G., Leenders F., Lu X., Fernández-Cuesta L., Bosco G., Müller C., Dahmen I., Jahchan N.S., Park K.S., Yang D., Karnezis A.N., Vaka D., Torres A., Wang M.S., Korbel J.O., Menon R., Chun S.M., Kim D., Wilkerson M., Hayes N., Engelmann D., Pützer B., Bos M., Michels S., Vlasic I., Seidel D., Pinther B., Schaub P., Becker C., Altmüller J., Yokota J., Kohno T., Iwakawa R., Tsuta K., Noguchi M., Muley T., Hoffmann H., Schnabel P.A., Petersen I., Chen Y., Soltermann A., Tischler V., Choi C.M., Kim Y.H., Massion P.P., Zou Y., Jovanovic D., Kontic M., Wright G.M., Russell P.A., Solomon B., Koch I., Lindner M., Muscarella L.A., la Torre A., Field J.K., Jakopovic M., Knezevic J., Castaños-Vélez E., Roz L., Pastorino U., Brustugun O.T., Lund-Iversen M., Thunnissen E., Köhler J., Schuler M., Botling J., Sandelin M., Sanchez-Cespedes M., Salvesen H.B., Achter V., Lang U., Bogus M., Schneider P.M., Zander T., Ansén S., Hallek M., Wolf J., Vingron M., Yatabe Y., Travis W.D., Nürnberg P., Reinhardt C., Perner S., Heukamp L., Büttner R., Haas S.A., Brambilla E., Peifer M., Sage J, & Thomas R.K. (2015). Comprehensive genomic profiles of small cell lung cancer. Nature, 524(7563), 47-53.

+ Open protocol

+ Expand

Cell Line Maintenance and Validation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human SCLC cell line SBC3 was gifted by Dr. Takashi Kijima (Osaka University, Japan), and SBC5 was obtained from the Japanese Collection of Research Bioresources (JCRB) Cell Bank, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan. BEAS-2B, DMS273, DMS53, NCI-H82, NCI-H526, NCI-H69 and Colo668 were originally obtained from ATCC (Rockville, MD, USA). All the cell lines were routinely maintained and cultured in standard culture conditions, in a CO₂ incubator at 37 °C, and regularly validated using STR profiling and checked for mycoplasma infections prior to experiments. For routine maintenance, SBC3, SBC5, NCI-H82, NCI-H69, NCI-H526, DMS273, DMS53, and Colo668 cells were cultured in RPMI-1640 medium supplemented with 1500 mg/L sodium bicarbonate, 10% fetal bovine serum (Sigma Cat#12303C), 1% penicillin/streptomycin cocktail solution (Invitrogen Cat#15,140–122), 1% sodium pyruvate (Invitrogen Cat#11,360,070), and 1% L-glutamine (Invitrogen Cat#25,030–081).

Khan P., Siddiqui J.A., Kshirsagar P.G., Venkata R.C., Maurya S.K., Mirzapoiazova T., Perumal N., Chaudhary S., Kanchan R.K., Fatima M., Khan M.A., Rehman A.U., Lakshmanan I., Mahapatra S., Talmon G.A., Kulkarni P., Ganti A.K., Jain M., Salgia R., Batra S.K, & Nasser M.W. (2023). MicroRNA-1 attenuates the growth and metastasis of small cell lung cancer through CXCR4/FOXM1/RRM2 axis. Molecular Cancer, 22, 1.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!