Cary 60 uv vis spectrometer

UV spectra were collected on a Cary 60 UV–vis spectrometer (Agilent) with Cary WinUV 5.1 software. Measurements were performed over a spectral range of 200–800 nm at a scan speed of 300 nm/min with 0.5 nm data intervals. The buffer solution baseline was automatically subtracted.
After collection of the first spectrum, mDna2 at 25 μM was incubated at 4 °C for 20 h, in a similar setup to the one used in EMSAs. After the collection of the spectrum after 20 h, TCEP was added to a final concentration of 57 mM and incubated for 30 min.

The USP paddle apparatus (Pharmatest, Steinheim, Germany) was used with a rotating speed of 50 rpm. For CBZ and TBM, samples of pure drug, physical mixes, or loaded MPs (size fraction 150–250 μm) were added to 500 mL of the respective dissolution medium maintained at a temperature of 37 °C. The aqueous solution was filtered and continuously pumped (12.5 mL/min) to a flow cell in a Cary 60 UV–Vis spectrometer (Agilent Technologies GmbH, Waldbronn, Germany) and absorbance values were recorded at 285 nm and 231 nm for CBZ and TMB, respectively. In case of CIN, samples of pure drug and loaded MPs (size fraction 150–250 μm) were added to 1 L of phosphate buffer solution pH 6.8 at 37 °C. At specific time points, 2 mL of the medium were withdrawn using an 8 µm filter to avoid the removal of the MPs and the amount of drug was quantified by HPLC. Two mL of fresh medium were added to keep the volume constant. The amount of solid dispersion (and physical mixtures) was 80-100 mg for samples containing CBZ and TBM, whereas 10–15 mg for samples containing CIN. The dissolution tests were performed on six samples.

UV–Vis spectroscopy was performed using a Cary 60 UV–Vis spectrometer (Agilent Technologies, Santa Clara, CA, USA). Transmission electron microscopy (TEM) and energy-dispersive X-ray (EDX) mapping were performed using an FEI Tecnai F20 transmission electron microscope (FEI Company, Eindhoven, the Netherlands). The hydrodynamic size of the nanoparticles was measured using ζ-potentials (ELS-Z, Otsuka, Japan). Fourier transform infrared (FT-IR) spectra were recorded on a PerkinElmer spectrometer in the range between 4000 and 400 cm⁻¹.