M. oryzae strains were grown in liquid YEG medium (Glucose 1 g, yeast extract 0.2 g in 100 ml of distilled water) for 3-days in dark at 200 rpm at 28 °C. Mycelia were filtered and genomic DNA was extracted using GenElute plant genomic Miniprep kit (Sigma Cat. No. G2N70-1KT). Total RNA was isolated from 3-days old mycelia on YEG liquid medium from MG01 using TRIzol method [13] (link).
Genelute plant genomic miniprep kit
Manufactured by Merck Group
Sourced in United States
The GenElute™ Plant Genomic Miniprep Kit is a laboratory product for extracting and purifying genomic DNA from a variety of plant species. It provides a simple and efficient method for isolating high-quality DNA that can be used in various downstream applications such as PCR, cloning, and sequencing.
Automatically generated - may contain errors
3 protocols using genelute plant genomic miniprep kit
1
Fungal Genomic DNA and RNA Extraction
2
Genotyping and Genetic Mapping Protocol
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Extraction of DNA was done using the GenElute™ Plant Genomic Miniprep Kit (Sigma-Aldrich, St. Louis, MO), and the quantity and quality was determined using Nanodrop spectrophotometer ND-1000 (Nanodrop Technologies, Wilmington, DE), electrophoresis on agarose gel (1.0%) and Qubit® dsDNA BR Assay Kit (Life Technologies, Eugene, OR).
Genotyping of the TAD×DUL RILs was based on GBS, and map construction were previously described by Oren et al. [39 (link)]. Map construction was based on 89 343 SNPs across 146 lines. SNP filtration were done with TASSEL v.5.2.43 [74 (link)] and linkage maps construction was done using the ASMap R package [75 (link)]. Genotyping of the GWAS180 diversity panel was performed using GBS, as described by Gur et al. [72 (link)] and the final SNP set included 23 931 informative SNPs across 177 accessions. DNA of the founder lines of the HDA20 population was extracted and shipped to the Genomic Diversity Facility at Cornell University (Ithaca, NY) for WGS to an estimated 30× coverage, yielding 4 million informative SNPs as previously described [57 (link)].
Genotyping of the TAD×DUL RILs was based on GBS, and map construction were previously described by Oren et al. [39 (link)]. Map construction was based on 89 343 SNPs across 146 lines. SNP filtration were done with TASSEL v.5.2.43 [74 (link)] and linkage maps construction was done using the ASMap R package [75 (link)]. Genotyping of the GWAS180 diversity panel was performed using GBS, as described by Gur et al. [72 (link)] and the final SNP set included 23 931 informative SNPs across 177 accessions. DNA of the founder lines of the HDA20 population was extracted and shipped to the Genomic Diversity Facility at Cornell University (Ithaca, NY) for WGS to an estimated 30× coverage, yielding 4 million informative SNPs as previously described [57 (link)].
3
Plant DNA Extraction and Quantification
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DNA isolations were performed using the GenElute™ Plant Genomic Miniprep Kit (Sigma-Aldrich, St. Louis, MO, USA). DNA quality and quantification were determined using a Nanodrop ND-1000 (Nanodrop Technologies, Wilmington, DE, USA) spectrophotometer, electrophoresis on an agarose gel (1.0%), and Qubit® dsDNA BR Assay Kit (Life Technologies, Eugene, OR, USA).
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