Human HNSCC cell lines SCC9, SCC15, SCC25, CAL27 and HEK293T cells were obtained from ATCC (Rockville, MD, USA). UM1 and UM-SCC1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA). HSC3, HSC6 and CAL33 were kindly provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA). The characteristics of HNSCC cell lines are described in Table S1 . The UM-SCC1, HSC3, HSC6, CAL27, CAL33 and HEK293T cells were cultivated in Dulbecco's modified Eagle's medium (DMEM, Gibco, Rockville, MD, USA) supplemented with 10% fetal bovine serum (FBS, Gibco). The SCC9, SCC15, SCC25 and UM1 cells were maintained in DMEM-F12 (Gibco) supplemented with 10% FBS. All cells were incubated at 37°C in a humidified atmosphere containing 5% CO2. All cell lines were routinely tested for Mycoplasma by PlasmoTestTM Mycoplasma contamination detection kit (InvivoGen).
Um scc1
Manufactured by Thermo Fisher Scientific
Sourced in United States
The UM-SCC1 is a laboratory equipment used for cell culture and other research applications. It is a squamous cell carcinoma cell line derived from the human upper aerodigestive tract.
Automatically generated - may contain errors
Lab products found in correlation
Cal27, by Thermo Fisher Scientific (4 mentions)
Cal27, by American Type Culture Collection (4 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
Hsc 3, by Thermo Fisher Scientific (2 mentions)
Dmem f12, by Thermo Fisher Scientific (2 mentions)
Plasmotesttm mycoplasma contamination detection kit, by InvivoGen (1 mentions)
Hek293t, by Thermo Fisher Scientific (1 mentions)
Scc25, by Thermo Fisher Scientific (1 mentions)
Scc15, by American Type Culture Collection (1 mentions)
Scc25, by American Type Culture Collection (1 mentions)
Scc15, by Thermo Fisher Scientific (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Defined keratinocyte sfm, by Thermo Fisher Scientific (1 mentions)
Hsc 3 cell line, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Scc 4, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Um scc1, by American Type Culture Collection (1 mentions)
Cal 33, by Thermo Fisher Scientific (1 mentions)
4 protocols using um scc1
1
Acquisition and Maintenance of HNSCC Cell Lines
2
Cell Line Characterization and Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Cal27 and FaDu cell lines were obtained from the ATCC (United States); the HSC-3 cell line was obtained from the JCRB Cell Bank (Japanese); the SCC4 cell line was purchased from the BeNa Culture Collection (BNCC, China); and the UM-SCC1 and human normal oral keratinocyte (NOK) cell lines were obtained from the State Key Laboratory of Oral Diseases. All cancer cell lines were authenticated by STR analysis. Cal27, FaDu, SCC4, UM-SCC1 and HSC-3 cells were cultured in DMEM (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum, while NOK cells were cultured in defined keratinocyte SFM (Gibco, Grand Island, NY, USA) with corresponding growth supplement. All cells were maintained at 37 °C in a humidified atmosphere with 5% CO2. For cellular intervention involving inhibitors or activators, all reagents were applied at specific final concentrations, which were diluted with dimethyl sulfoxide (DMSO). An equivalent volume of DMSO was added as a negative control. The final concentrations are indicated as described in the figure legends.
3
HNSCC Cell Lines and PI3K Inhibitor
4
Silencing YKT6 in Oral Squamous Cell Carcinoma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A total of three cell lines including human OSCC CAL-27 (American Type Culture Collection (ATCC), Zhong Qiao Xin Zhou Biotechnology, Shanghai, China), CAL-33 (Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun, China) and UM-SCC-1 (ATCC, Cellcook Biotechnology, Guangzhou, China) were used in current research. CAL-27 and CAL-33 were cultured in DMEM (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (Gibco, Carlsbad, CA, USA). UM-SCC-1 was cultured in RPMI-1640 (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (Gibco, Carlsbad, CA, USA). The culture was maintained in a humidified incubator with 37°C, 5% CO2. Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) was used to transfect Negative Control (NC) and YKT6 siRNAs (RiboBio, Guangzhou, China) into OSCC cells according to the manufacturer’s instruction. Target sequences for YKT6 siRNAs were GAACTAGATGAGACCAAAA (YKT6 si1) and GGATGGTCACCTCAGTAGA (YKT6 si2).
The primer sequences for qRT-PCR were listed as follows: hβ-ACTIN, Forward (F): 5′-AGTTGCGTTACACCCTTTCTTG-3′, Reverse (R): 5′-CACCTTCACCGTTCCAGTTTT-3′; hYKT6, Forward (F): 5′-CAGCGTCCTCTACAAAGGCG-3′, Reverse (R): 5′-ACAATCAGTTGACTCGTGAAGG-3′.
The primer sequences for qRT-PCR were listed as follows: hβ-ACTIN, Forward (F): 5′-AGTTGCGTTACACCCTTTCTTG-3′, Reverse (R): 5′-CACCTTCACCGTTCCAGTTTT-3′; hYKT6, Forward (F): 5′-CAGCGTCCTCTACAAAGGCG-3′, Reverse (R): 5′-ACAATCAGTTGACTCGTGAAGG-3′.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!