Nmri nu nu mice
The NMRI nu/nu mice are a laboratory mouse strain that lack a functional thymus, resulting in a deficiency in T cells. These mice are often used in research applications that require an animal model with a compromised immune system.
Lab products found in correlation
16 protocols using nmri nu nu mice
Preclinical cancer models for physical activity
Therapeutic MSC and Bortezomib for Colo205 Tumors
In Vivo Xenograft Experiments in Immunodepressed Mice
Subcutaneous Xenograft Mouse Model
Metformin and GANT61 Enhance Radiotherapy
Tumorigenicity Assay of OVCAR-5 Cells
Establishing Xenograft Models of Liposarcoma
The SW872 liposarcoma cell line (Cell Lines Service, Eppelheim, Germany) was cultured in Dulbecco's modified Eagle's medium/F12 medium with 10% FBS (all from Life Technologies). The SW872 cell line has been previously studied in vitro and in vivo[15] (link), [16] (link). The SW872 model was generated by subcutaneous, bilateral injection of 5 × 106 cells per mouse site. Patient-derived DDLPS xenografts (UZLX-STS3 and UZLX-STS5) were established by bilateral subcutaneous implantation of fresh surgically resected tumor specimens from patients with DDLPS. Tumor tissue was further re-transplanted from mouse to mouse at least twice. From each passage, tumor fragments were collected for histologic and molecular characterization.
Pharmacokinetics of Anti-Proliferative Agent
were conducted in accordance with the German Animal Welfare Law and
were approved by local authorities. Female NMRI nu/nu mice (Janvier,
France) were dosed once at 100 mg/kg orally (p.o., in PEG400/ethanol 9:1) (n = 3 per group), intraperitoneally
(i.p., in solutol/ethanol/water 4:1:5), or subcutaneously
(s.c., in castoroil/benzylbenzoate 9:1). After 0.5,
3, 6, 14, 24, and 48 h, mice were sacrificed by decapitation and blood
sampled in potassium-EDTA tubes (Sarstedt, Germany). 100 μL
of plasma was used for analysis. Samples were precipitated by the
immediate administration of ice cold acetonitrile in a 1:5 dilution.
Samples were frozen at −20 °C overnight and subsequently
centrifuged at 3000 rpm for 15 min. The supernatant was analyzed with
an Agilent 1200 HPLC system with MS/MS detection (AB Sciex, Framingham,
MA). Obtained exposures were corrected for plasma–protein binding
and reported in relation to the antiproliferative IC50u.
Mouse Embryo Development Protocol
Xenograft Engraftment in Nude Mice
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