The microstructures of the different kinds of nanoparticles were observed by a transmission electron microscope (JEOL, JEM-2100). The microstructures of the derived SCPs were observed by a scanning electron microscope (Hitachi S–300 N). The optical images of the generated SCPs were captured by a stereomicroscope (NOVEL NTB-3A, Ningbo Yongxin Optics Co., Ltd., China) equipped with a color CCD camera (Media Cybernetics Evolution MP 5.0 RTV). The reflection spectra of the generated SCPs were obtained through an optical microscope (Olympus BX51) equipped with a fiberoptic spectrometer (Ocean Optics QE65000). The fluorescent intensity curves were obtained through a fluorescent microscope (Olympus, CKX41) equipped with a fiberoptic spectrometer (Ocean Optics QE65000). The fluorescent images were captured by a stereoscopic microscope (Olympus, SZX16) equipped with the camera under a mercury lamp (Olympus, U-RFL-T) and a halogen lamp (Olympus, LG-PS2).
Qe65000
Manufactured by OceanOptics
Sourced in United States, Germany
The QE65000 is a high-performance spectrometer from Ocean Optics. It features a 2048-element linear silicon CCD-array detector with a wide spectral range and high sensitivity. The spectrometer is designed for laboratory applications requiring accurate and reliable spectral measurements.
Automatically generated - may contain errors
Lab products found in correlation
Ls 1 cal int, by OceanOptics (5 mentions)
Dh 2000, by OceanOptics (4 mentions)
Bx51 microscope, by Olympus (3 mentions)
Beamsplitter bsn11, by Thorlabs (3 mentions)
Fois 1, by OceanOptics (3 mentions)
Nir quest, by OceanOptics (3 mentions)
Spectrasuit, by OceanOptics (2 mentions)
Dh 2000 bal, by OceanOptics (2 mentions)
Pda36a2, by Thorlabs (2 mentions)
Mplanfl, by Olympus (2 mentions)
Cf p20s, by Olympus (2 mentions)
Matlab, by MathWorks (2 mentions)
Polarizer gth10m b, by Thorlabs (2 mentions)
Escalab 250xi, by Thermo Fisher Scientific (2 mentions)
Spectrasuite, by OceanOptics (1 mentions)
Kl 2500, by Schott (1 mentions)
Nanosem650, by Thermo Fisher Scientific (1 mentions)
Glan thompson polarizer gth10m b, by Thorlabs (1 mentions)
Lg ps2, by Olympus (1 mentions)
U rfl t, by Olympus (1 mentions)
75 protocols using qe65000
1
Characterization of Nanoparticle Microstructures
2
Hyperspectral Imaging with Linear Unmixing
3
Hyperspectral Image Analysis Using Linear Unmixing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hyperspectral image data were analyzed using non-negatively constrained linear unmixing, as described previously 23 (algorithms available at https://www.southalabama.edu/centers/bioimaging/resources.html). Briefly, pure samples of each spectral component were imaged: Cal-590, Tiny Beads, Big Beands, and unlabeled cells for autofluorescence. Images were corrected to a flat spectral response using measurements of excitation illumination intensity at the microscope stage as recorded by a fiber-coupled spectrometer (QE65000, Ocean Optics) and integrating sphere (FOIS-1, Ocean Optics), calibrated to a NIST-traceable illumination source (LS-1-CAL-INT, QE65000, Ocean Optics). Corrected hyperspectral images of single-label samples were then processed to build a spectral library by selecting a region within each image of high signal level and extracting the pixel-averaged spectrum. Spectra were normalized to a peak value of unity and stored in a spectral library. Timelapse hyperspectral image data were then analyzed utilizing a least-squares non-negatively constrained regression algorithm and the spectral library. Unmixed images were utilized for further analysis.
4
Second Harmonic Generation in MF-LNOI
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The schematic of our experimental setup is shown in Fig. 4(a) . A tapered MF was fixed at and precisely positioned by two xyz-stages. An optical microscope with a CCD camera was used to monitor MF and LNOI waveguide. At one end of the MF, a tunable laser (Santec TSL210) launched light into the sample through an in-line polarization controller. At the other end, a highly-sensitive visible spectrometer (Ocean Optics QE65000) and a power meter were used to measure the SH wavelength and power. Since the spectrometer only responds in the visible, we had not used filter to remove the pump light. Before measurement, the spectrometer was carefully calibrated with the power meter. The normalized SHG efficiencies inside the MF-LNOI structures were calculated by taking into account the in/out-coupling losses, as illustrated in Supplementary Fig. S2 .
5
Light Penetration in Coastal Microbial Mats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Light penetration in the coastal microbial mat was measured with a scalar irradiance microsensor (Lassen et al., 1992 (link); Kühl et al., 1997 (link); Kühl, 2005 (link)) connected to a sensitive fiber-optic spectrometer (QE65000, Ocean Optics, USA) that was interfaced to a PC running dedicated spectral acquisition software (Spectrasuite, Ocean Optics, USA). Mat samples were illuminated vertically from above with a fiber-optic halogen lamp equipped with a collimating lens (KL-2500, Schott, Germany), where the downwelling photon irradiance was regulated with a built-in neutral density screen to 500 μmol photons m-2 s-1. A scalar irradiance microsensor was mounted in a manually operated micromanipulator (MM33, Märtzhäuser GmbH, Germany) and inserted into the mat at a 45° angle relative to the vertically incident light. Measurements were corrected for the measuring angle, and depths are given as vertical depth below the mat surface. Data were normalized to the incident downwelling irradiance as measured with the scalar irradiance microsensor positioned in the light path at similar distance as the mat surface but over a black light absorbing well.
6
Optical Sensing System for Antigen-Antibody Reactions
Check if the same lab product or an alternative is used in the 5 most similar protocols
Figure 2 shows the optical measurement system comprised a white-light source (DH-2000-BAL, Ocean Optics Inc., Dunedin, FL, USA), reflectance optical probe (QD-400, Ocean Optics, Inc.), antigen-antibody reaction chamber made of Teflon, and a spectrometer (QE65000, Ocean Optics, Inc.). The spectrometer, which could measure absorbance and reflectance, was operated over a wide wavelength (200–1100 nm) with high resolution (0.17 nm). During the sensing process, we measure the reflected light from the fabricated Al sheet that had undergone an antigen-antibody reaction. As shown in Figure 2 , the perpendicularly-irradiated light on the AAO chip was reflected from the AAO chip and the reflected light was collected through the same optical probe that had one receiver in the center and six sources surrounding the receiver. The collected light was analyzed with spectrum analysis software (spectrasuit, Ocean Optics, Inc.) in real-time.
+ Expand
7
LSPR Optical Platform Assembly
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The LSPR optical platform was self-assembled in the laboratory (Fig. S2 ). The LSPR extinction spectra were obtained using a spectrometer (QE65000, Ocean Optics, USA). Scanning electron microscopy (SEM) images were obtained using aby field emission scanning electron microscope (NanoSEM650, FEI, USA). Fluorescence images were obtained using an optical microscope (Ti-s, Nikon, Japan).
8
Raman Spectroscopy of Biological Cells
9
Spectroscopic Analysis of Oxidized Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Data were acquired using Spectrasuite software and a fibre optic system comprising a cooled CCD (QE65000, 25 μm slit width), a tungsten halogen light source (HL-2000-FHSA-HP), 1000 μm optical fibres (QP1000-2-VIS-BX) and either a standard cuvette holder (CUV) (all from Ocean Optics) or a light-integrating sphere with centre mounted cuvette holder (Labsphere, Prolite). Unless otherwise stated the oxidised spectrum was recorded and stored with subsequent spectra acquired as difference spectra relative to oxidised.
10
Fabrication and Characterization of Silicon Nanocylinders
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The silicon cylinders were fabricated using an etching mask of ZEP-520 electron beam resist patterned by electron beam lithography and subsequent inductively coupled plasma etching on a Soitec SOI wafer with a 220 nm thick Si layer on 2 μm buried oxide. The fabrication is schematically shown in Fig. 9a .
Scattering spectra of the fabricated Si nanocylinders were measured using optical dark-field spectroscopy. The spectroscopic set-up (Fig 9b ) included a BX51 microscope (Olympus) equipped with a halogen light source and a fibre-coupled grating spectrometer QE65000 (Ocean Optics). In these measurements a single Si nanoparticle is irradiated at highly oblique angles around 70 deg. and back scattered light collected from angles up to 64 deg. determined by the numerical aperture (NA) of the x100 microscope objective MPLFLNBDP (Olympus). The microscope images were captured with a XC30 digital color camera (Olympus).
Scattering spectra of the fabricated Si nanocylinders were measured using optical dark-field spectroscopy. The spectroscopic set-up (
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!