Nitric oxide kit

The dried fruiting bodies of Armillaria mellea (Vahl) P. Kumm. were purchased from Antu Xinyu Edible and Medicinal Fungus Research and Development Co., Ltd. (Yanbian, China). Armillaria mellea colonies were artificially cultivated in the planting base of the company. The fresh fruiting bodies were collected from July to August every year, cleaned with water, and dried in the sun. In addition, 90 male Kunming mice, 4–5 weeks old, and weighing 15 ± 2 g, were purchased from Experimental Animal Center, Jilin University, and the mice were raised in separate cages at 22–25°C in a light-dark cycle of 12 h, with access to food and water ad libitum. Superoxide dismutase (SOD) and catalase (CAT) kits were purchased from the Nanjing Jiancheng Bioengineering Institute (China); the nitric oxide (NO) kit was from Beyotime Biotechnology Company (China); NE, DA hydrochloride, 5-HT hydrochloride, and bromodeoxyuridine (BrdU) markers were obtained from Sigma-Aldrich, United States; all monoclonal antibodies and the immunofluorescence secondary antibodies were purchased from Abcam, England; and the high-performance liquid chromatography (HPLC) grade methanol and trisodium citrate were delivered by Merck, Germany. Other reagents and deionized water were of analytical grade.

Catechin was purchased from Solarbio Science & Technology Co.LTD (Beijing, China). Lipopolysaccharide (LPS) was purchased from Yuanye Bio-Technology Co.LTD (Shanghai, China). Macrophage RAW264.7 cells stored in liquid nitrogen were purchased from Jinan University (Guangzhou, China). Dulbecco Modified Eagle Medium (DMEM) was purchased from Thermo Fisher Scientific Inc (Thermo Fisher, Waltham, MA, USA). Phosphate buffer solution (PBS), penicillin-streptomycin and fetal bovine serum (FBS) were supplied by Gibco Life Technologies (Grand Island, NY, USA). Cell Counting Kit-8 (CCK-8) whose product code was CK04 was purchased from Dongren Chemical Technology Co. LTD (Dongying, China). Nitric oxide (NO) kit whose product code was S0021S was purchased from Beyotime Biotechnology Co. LTD (Shanghai, China). Aspirin was purchased from Solarbio Science & Technology Co.LTD (Beijing, China). Other mentioned chemical materials were all purchased from Guangzhou Chemical Co. LTD (Guangzhou, China). All other reagents used in our research were of analytical grade.