Sa3800 spectral analyzer

Manufactured by Sony

Sourced in Japan, United States

The SA3800 Spectral Analyzer is a laboratory equipment designed to analyze the frequency spectrum of various signals. It is capable of measuring the power distribution across different frequency bands, providing users with detailed information about the spectral characteristics of the input signal.

Automatically generated - may contain errors

Lab products found in correlation

Sa3800 2, by Sony (3 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (2 mentions) Annexin 5 fitc pi apoptosis kit, by Abnova (1 mentions) Zymolyase 20t, by US Biological (1 mentions) Lsrfortessa, by BD (1 mentions) Facsaria 3, by BD (1 mentions) Facscalibur, by BD (1 mentions) Il 18, by Sino Biological (1 mentions) Opteia, by BD (1 mentions) Ficoll paque solution, by Axis-Shield (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Mouse igg1 kappa, by Thermo Fisher Scientific (1 mentions) Sodium azide, by Merck Group (1 mentions) Anti cd61 pe clone 2c9 g2, by BioLegend (1 mentions) Microtainer blood collection tube, by BD (1 mentions) Countbright counting beads, by Thermo Fisher Scientific (1 mentions) Clone htk888, by BioLegend (1 mentions)

39 protocols using sa3800 spectral analyzer

Binding of Borrelia Peptidoglycan to PGLYRP1 and CD55

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Low passage B. burgdorferi were cultured to a density of ~10⁶−10⁷ cells/mL, washed two times with PBS and incubated with either recombinant human PGLYRP1 (with 8X-His tag), recombinant mouse PGLYRP1 (with 6X-His tag) (Sino Biological, #50115-M08H), recombinant human CD55 (with 8X-His tag), or murine CD55 (with 8X-His tag) at room temperature for 1 hour. Varying concentrations of purified B. burgdorferi PG [11 (link)] were used for this assay. PG preparation had been sonicated prior to use and consisted of fragmented sacculi. Borrelia PG was pre-incubated with either murine or human PGLYRP1 and was subsequently added to Borrelia to test for binding. After a co-incubation period of 1 hour, spirochetes were fixed in 4% PFA, washed three times with PBS and blocked in 1% BSA overnight at 4°C. The spirochetes were probed anti 6X-His monoclonal antibody- conjugated to Alexa Fluor 488 (ThermoFisher, #MA1-21315-488) and run through SA3800 Spectral Analyzer (Sony Biotechnology). The data was analyzed by FlowJo.

Gupta A., Arora G., Rosen C.E., Kloos Z., Cao Y., Cerny J., Sajid A., Hoornstra D., Golovchenko M., Rudenko N., Munderloh U., Hovius J.W., Booth C.J., Jacobs-Wagner C., Palm N.W., Ring A.M, & Fikrig E. (2020). A human secretome library screen reveals a role for Peptidoglycan Recognition Protein 1 in Lyme borreliosis. PLoS Pathogens, 16(11), e1009030.

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Mitochondrial Mass Estimation in Candida

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Mitochondrial mass/volume was estimated using MitoTracker Green fluorescence.^[⁷⁰^]C. albicans cell suspensions (≈10⁶ CFU mL⁻¹) were treated with DMSO or MMs (0.5–2 × MIC) and then irradiated with 405‐nm light (87.6 J cm⁻²). The cells were then stained with MitoTracker Green (200 nm) for 30 min at 30 °C and washed three times with PBS. At least 10 000 cells per sample were analyzed in an SA3800 Spectral Analyzer (Sony Biotechnology, CA, USA).

Santos A.L., Beckham J.L., Liu D., Li G., van Venrooy A., Oliver A., Tegos G.P, & Tour J.M. (2023). Visible‐Light‐Activated Molecular Machines Kill Fungi by Necrosis Following Mitochondrial Dysfunction and Calcium Overload. Advanced Science, 10(10), 2205781.

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Annexin V-FITC/PI Assay for Candida Apoptosis

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The occurrence of necrosis and apoptosis was investigated using an Annexin V‐FITC/PI assay.^[²⁴^]C. albicans cells were grown as described for time‐kill experiments, washed in sorbitol buffer (0.5 mm MgCl₂, 35 mm potassium phosphate, pH 6.8, containing 1.2 m sorbitol), and resuspended in the same buffer containing zymolyase 20 T (20 mg mL⁻¹, US Biological Life Sciences, MA, USA). After 1 h of digestion at 30 °C, protoplasts were centrifuged, washed, and resuspended in binding buffer (140 mm NaCl, 10 mm HEPES, 2.5 mm CaCl₂, 1.2 m sorbitol, pH 7.4). Protoplasts were treated with 1% DMSO or MMs (0.5–2 × MIC) and then irradiated with 405‐nm light (87.6 J cm⁻²). The protoplasts were immediately labeled using an Annexin V‐FITC/PI Apoptosis Kit (Abnova, Taiwan) per the distributors' instructions. At least 10 000 cells per sample were analyzed in an SA3800 spectral analyzer (Sony Biotechnology, CA, USA).

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Isolation and Analysis of Mouse Splenic Lymphocytes

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Mononuclear cells from the mouse spleen were obtained by mashing through a 70-µm cell strainer with a syringe plunger. Cells suspensions were washed in PBS and resuspended in 1 ml of red blood cell lysis (155 mM NH₄Cl, 10 mM KHCO₃, 0.1 mM EDTA) for 5 min at room temperature and washed. Cells were resuspended in PBS 1% BSA, stained with combinations of antibodies listed in Table S2 for analysis of different lymphocyte populations, and passed through a 40-μm nylon cell strainer before acquisition. NP-specific cells were detected using NP₂₈-PE (N-5070-1; Biosearch Technologies). Data were acquired using BD LSR Fortessa, BD Facscalibur (BD Biosciences), or SA3800 Spectral Analyzer (Sony Biotechnology) and analyzed using FlowJo (BD Biosciences). Sorting was done with a BD FACSARIA III (BD Biosciences) apparatus.

Litzler L.C., Zahn A., Dionne K.L., Sprumont A., Ferreira S.R., Slattery M.R., Methot S.P., Patenaude A.M., Hébert S., Kabir N., Subramani P.G., Jung S., Richard S., Kleinman C.L, & Di Noia J.M. (2023). Protein arginine methyltransferase 1 regulates B cell fate after positive selection in the germinal center in mice. The Journal of Experimental Medicine, 220(9), e20220381.

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DNA Damage Response Assay with Rucaparib

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2.5x10⁵ RPE1-hTERT cells were plated in 6 cm plates and allowed to adhere for 24 h. Cells then received a two-hour pre-treatment with either 10 μM rucaparib or DMSO vehicle control. After 2 h, DSBs were induced by the addition of either a low dose (10ng/ml) or high dose (400ng/ml) of NCS. Cells were allowed to grow for either 24 or 48 h. At this time, media was removed and placed into a separate tube. Cells were removed from plate by trypsinization. Once detached, cells were rinsed in media and placed into the same tube as the original growth media. Cells were pelleted by 400x g spin for 4 min and washed twice in PBS. Cells were then resuspended in 300 μl PBS and passed through a 45 μM cell strainer to remove clumps. Cells were then fixed by the addition of 700 μL cold EtOH dropwise while vortexing. Fixed cells were stored at −20°C until experiment. Cells were prepared for analysis by pelleting cells with a 750x g spin for 5 min and two washes in 1 mL PBS. 1x10⁶ cells were then resuspended in a staining solution containing 20 μg/ml propidium iodide and 0.5 mg/ml RNaseA. Cells were incubated at 37°C for 30 min, then brought to 500 μl final volume and passed through a 45 μM mesh. Cells were analyzed using a Sony SA3800 Spectral Analyzer in the Flow Cytometry core facility (NIH Center for Cancer Research, Laboratory of Genome Integrity).

Hanson R.L, & Batchelor E. (2020). Rucaparib Treatment Alters p53 Oscillations in Single Cells to Enhance DNA-Double-Strand-Break-Induced Cell Cycle Arrest. Cell reports, 33(2), 108240.

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Inflammatory Cytokine Profiling in BALF

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BAL fluid (BALF) was obtained after 2.5 h MV with 0.5 mL of PBS with 2 mM EDTA by inserting a standard disposable intravenous catheter (BD Insyte Autoguard, 20GA 1.00 in., Becton Dickinson Infusion Therapy Systems Inc.) into the trachea. Cells were isolated from supernatant and analyzed for cell counts (Cellometer Auto2000, Nexcelcom Bioscience, Lawrence, MA) and differentiation by flow cytometry (SA3800 Spectral Analyzer, Sony, Tokyo, Japan). The levels of albumin and cytokines in BALF were measured using commercially available ELISA kits (albumin: Bethyl Laboratories, Montgomery, TX, USA; IL-1α: ELISA MAX, BioLegend, San Diego, CA, USA; IL-1β and TNF-α: eBioscience; IL-6: BD OptEIA, Becton Dickinson; IL-18: Sino Biological, Beijing, China) as previously described (12 (link)).

Nosaka N., Martinon D., Moreira D., Crother T.R., Arditi M, & Shimada K. (2020). Autophagy Protects Against Developing Increased Lung Permeability and Hypoxemia by Down Regulating Inflammasome Activity and IL-1β in LPS Plus Mechanical Ventilation-Induced Acute Lung Injury. Frontiers in Immunology, 11, 207.

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Isolation of PMNs and PBMCs from Bovine Blood

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PMNs and PBMCs were isolated from the blood collected from cows immediately before (0 h) or 6 h after AI, as previously described [17 (link)]. Briefly, blood
samples were mixed with an equal volume of PBS free from calcium and magnesium (PBS^–/–), slowly layered over Ficoll-Paque solution (Lymphoprep, Axis-Shield, Norway), and
centrifuged at 1000 g for 35 min at 10°C. The white buffy coat layer (PBMCs) and the lower PMN layer were collected, mixed with a double volume of hemolysis buffer
(NH₄Cl 155 mM, KHCO₃ 9.9 mM, EDTA 96.7 μM) for 3 min, and centrifuged at 500 × g for 5 min at 10°C to remove red blood cells. After centrifugation,
cell pellets were washed twice with PBS^–/–. The purity of PBMCs and PMNs was > 95% and > 97%, respectively, as evaluated using the SA3800
Spectral Analyzer (Sony Biotechnology, Tokyo, Japan). The viability, as assessed by Trypan blue staining, was > 96% and > 98%, respectively. The cells
were then collected, lysed using Trizol (Invitrogen, Carlsbad, CA, USA), and stored at –80°C until RNA extraction.

MAREY M.A., MA D., YOSHINO H., ELESH I.F., ZINNAH M.A., FIORENZA M.F., MORIYASU S, & MIYAMOTO A. (2023). Sperm induce proinflammatory responses in the uterus and peripheral blood immune cells of artificially inseminated cows. The Journal of Reproduction and Development, 69(2), 95-102.

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Quantitative DNA Analysis of SPM Cells

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Cells in 1 ml of SPM culture were collected by centrifugation. The pellet was then resuspended in 1 ml of 70% ethanol. Fixed cells were recollected and washed by suspending 1 ml of 50 mM sodium citrate buffer. The collected cells were resuspended in 0.5 ml of sodium citrate buffer and sonicated for the 20 s at room temperature. Sonicated cells were incubated with 12.5 μl of 10 mg/mL RNAseA for 1 h at 50 °C and in 25 μl of 15 mg/ml proteinase K for 1 h at 50 °C. 0.5 ml of 16 μlg/ml PI solution was added and incubated for 30 min at room temperature in the dark. The samples were then analyzed using SA-3800 Spectral analyzer (Sony). Floreada.io was used for gating and plotting graphs.

Sampathkumar A., Zhong C., Tang Y., Fujita Y., Ito M, & Shinohara A. (2024). Replication protein-A, RPA, plays a pivotal role in the maintenance of recombination checkpoint in yeast meiosis. Scientific Reports, 14, 9550.

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Flow Cytometric Analysis of Cell-Bound Antibodies

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Antibodies were incubated with suspended cells (2.5 × 10⁵ cells per well) in a 96-well plate at 4 °C for 1 hour in FACS buffer consisting of PBS (K D Medical, MD, USA), 2mM EDTA (K D Medical, MD, USA), 1% BSA (Sigma-Aldrich, MO, USA) and 0.1% sodium azide (Sigma-Aldrich, MO, USA). Bound antibodies were detected with R-phycoerythrin conjugated F(ab’)2 goat anti-mouse IgG Fcγ (Cat # 115-116-071; Jackson ImmunoResearch, PA, USA) at 1:250 dilution for 45-60 min at 4°C. Mouse IgG1 kappa and mouse IgG2b kappa (Cat# 14-4714-82 and 14-4732-82 respectively; Invitrogen, NY, USA) antibodies were used as isotype controls. Antibody binding was characterized with the SA3800 Spectral Analyzer (Sony Biotechnology, San Jose, CA, USA), the data were analyzed with FlowJo (Tree Star, Inc., Ashland, OR, USA) and displayed in histogram format with the median fluorescence intensity plotted. All flow cytometry experiments were repeated at least once.

Ho E.C., Antignani A., Sarnovsky R, & FitzGerald D. (2019). Characterization of monoclonal antibodies generated to the 287–302 amino acid loop of the human epidermal growth factor receptor. Antibody Therapeutics, 2(4), 88-98.

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Quantifying T Cell Trafficking In Vivo

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SCID mice were injected intravenously with 10⁷ T cells or BsAb-armed T cells and then sacrificed after 24, 48, 72, or 96 h. The lymphocytes and splenocytes were collected and incubated with FITC-conjugated CD8a monoclonal antibody and phycoerythrin (PE)-conjugated goat anti-human IgG Fab antibody. The surface fluorescence was measured by a flow cytometer (Sony SA3800 Spectral Analyzer), and fluorescence intensities were analyzed using the SA3800 software program (Sony Biotechnology Inc, San Jose, CA).

Chen Y.J., Chen M., Cheng T.L., Tsai Y.S., Wang C.H., Chen C.Y., Wu T.Y., Tzou S.C., Wang K.H., Cheng J.J., Kao A.P., Lin S.Y, & Chuang K.H. (2023). A non-genetic engineering platform for rapidly generating and expanding cancer-specific armed T cells. Journal of Biomedical Science, 30, 35.

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