Vitros 950 at

Using blood samples collected in fluorinated tubes, the fasting blood glucose level was measured using a glucose oxidase method (VITROS 950 AT; Johnson & Johnson). Insulin was assayed using chemiluminescence (Advia Centaur; Bayer Corporation). Total cholesterol, HDL, and TG levels were analyzed using chemistry slides (VITROS DT; Johnson & Johnson). A measure of IR was estimated by the homeostatic model assessment of IR (HOMA-IR), calculated as the fasting blood glucose level (in milligrams per deciliter) times the fasting insulin level (in micro international units per milliliter), divided by 405.^{22 (link)}