Bongkrekic acid

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Bongkrekic acid is a laboratory reagent used in cellular and biochemical research. It functions as an inhibitor of the mitochondrial ADP/ATP carrier protein, which is involved in the exchange of ADP and ATP across the mitochondrial membrane. This property makes it a useful tool for studying mitochondrial processes and energy metabolism.

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Lab products found in correlation

Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyl tetrazolium bromide mtt, by Roche (1 mentions) Anti cyto c, by Thermo Fisher Scientific (1 mentions) Anti aif, by Cell Signaling Technology (1 mentions) α tubulin, by Novus Biologicals (1 mentions) Anti cox 4, by Santa Cruz Biotechnology (1 mentions) Anti bax n 20, by Santa Cruz Biotechnology (1 mentions) Anti bax, by Merck Group (1 mentions) Anti cleaved parp, by Merck Group (1 mentions) Anti survivin, by Novus Biologicals (1 mentions) Cyclosporine a, by Merck Group (1 mentions) Anti caspase 3, by Cell Signaling Technology (1 mentions) Anti bcl 2, by Abcam (1 mentions) N acetylcysteine, by Merck Group (1 mentions) Anti β actin, by Abcam (1 mentions) Pyruvate kinase, by Oriental Yeast (1 mentions) Lactate dehydrogenase, by Oriental Yeast (1 mentions) Rat tail collagen type 1, by Corning (1 mentions) Glutamaxtm, by Thermo Fisher Scientific (1 mentions) William s medium e wme, by Thermo Fisher Scientific (1 mentions)

2 protocols using bongkrekic acid

Apoptosis Induction and Mitochondrial Dysfunction

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The following reagents and antibodies were used: Bongkrekic acid (Santa Cruz Biotechnology, La Jolla, CA), JC-1 (eBioscience, San Diego, CA), Cyclosporine A, 2′,7′-dichlorofluorescein acetate (DCFH-DA), N-acetyl cysteine (Sigma, St.Louis, MO), Dioleylphosphatidylserine (Avanti Lipids, Alabaster, AL), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) ((Roche Diagnostics, Indianapolis, IN), and disuccinyl suberate (Thermo Scientific Fischer, Rockford, IL). Anti-Bcl-2, anti-β-Actin (Abcam, Cambridge, MA), anti-Cyto c (eBioscience, San Diego, CA), anti-AIF, anti-caspase-3, anti-cleaved caspase-3 (Cell Signaling Technology, Boston, MA, anti-Survivin, Smac/Diablo, α-Tubulin (Novus biological, Littleton, CO), anti-COX-4, anti-Bax (N-20; Santa Cruz Biotechnology, La Jolla, CA), anti-Bax (polymer-recognizing A67 clone; Sigma, St.Louis, MO) and anti- cleaved PARP (Millipore, Bedford, MA).
Animal maintenance and experimental procedures were carried out in accordance with the US National Institute of health Guidelines for Use of Experimental Animals and approved by the Institutional Animal Care and Use Committee of the University of Cincinnati and Cincinnati Children's Hospital Medical Center.

Sulaiman M.K., Chu Z., Blanco V.M., Vallabhapurapu S.D., Franco R.S, & Qi X. (2015). SapC-DOPS nanovesicles induce Smac- and Bax-dependent apoptosis through mitochondrial activation in neuroblastomas. Molecular Cancer, 14, 78.

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Isolation and Characterization of Mitochondria

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LPS from Escherichia coli O127:B8 (lot number: 0000082455, 1.5 × 10 6 endotoxin units/mg), perchloric acid, thiobarbituric acid, antimycin A, decylubiquinone, rotenone, dichloroindophenol, oligomycin, and rhodamine 123 were purchased from Millipore-Sigma (St. Louis, MO, USA). Cytochrome c, 1,1,3,3-tetraethoxypropane, cyclosporin A, collagenase, dexamethasone, and insulin were purchased from FUJIFILM Wako Pure Chemical Industries Ltd. (Osaka, Japan). ADP, ATP, pyruvate kinase, and lactate dehydrogenase were purchased from Oriental Yeast (Tokyo, Japan). Carboxyatractyloside and bongkrekic acid were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). William's medium E (WME) and GlutaMAX TM were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Fetal bovine serum (FBS) was purchased from Biosera (Nuaille, France). Antibiotic-antimycotic solutions were purchased from Nacalai Tesque Inc. (Kyoto, Japan). Type I collagen (rat tail) was purchased from Corning (Bedford, MA, USA).

Nakajima R., Takemura A., Ikeyama Y, & Ito K. (2023). Lipopolysaccharide administration increases the susceptibility of mitochondrial permeability transition pore opening via altering adenine nucleotide translocase conformation in the mouse liver. The Journal of toxicological sciences, 48(2).

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