Mouse or rabbit igg

Manufactured by Merck Group

Mouse or rabbit IgG is a type of immunoglobulin (Ig) used as a laboratory reagent. IgG is the most abundant antibody isotype in the body and plays a crucial role in the adaptive immune response. This product can be used as a control or reference standard in various immunological assays and techniques.

Automatically generated - may contain errors

Lab products found in correlation

Anti cyclin e antibodies, by Santa Cruz Biotechnology (1 mentions) Ab18251, by Abcam (1 mentions) Nbp2 12812, by Novus Biologicals (1 mentions) Irdye 800cw goat anti rabbit, by LI COR (1 mentions) Sc 9996, by Santa Cruz Biotechnology (1 mentions) A21057, by Thermo Fisher Scientific (1 mentions) A21070, by Thermo Fisher Scientific (1 mentions) T6074, by Merck Group (1 mentions) F1804, by Merck Group (1 mentions) A28175, by Thermo Fisher Scientific (1 mentions) Irdye 800cw goat anti mouse, by LI COR (1 mentions) Irdye 680rd goat anti rabbit, by LI COR (1 mentions)

Close spelling variants of this product

IgG rabbit IgG mouse

2 protocols using mouse or rabbit igg

Antibody Characterization for Cell Signaling

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Antibodies against CDK2, CDK6, cyclin D1, cyclin B, myc, HA, and Flag were purchased from MBL (Nagoya, Japan). Rabbit anti-Cullin1, anti-Skp1, and anti-cyclin E antibodies were from Santa Cruz (Dallas, TX, USA). Mouse anti-Skp1 and anti-Cullin1 antibodies were from Invitrogen (Carlsbad, CA, USA). Anti-Fbw7 antibody was from Sigma. Anti-Rbx1 antibody was from Cell Signaling Technology (Beverly, MA, USA). Anti-MAGE-C2 antibodies have been described previously (25). Antibodies specific for GAPDH, GFP and GST were from ProteinTech (Chicago, Illinois, USA). Mouse or rabbit IgG (Sigma) was used as control.

Hao J., Song X., Wang J., Guo C., Li Y., Li B., Zhang Y, & Yin Y. (2015). Cancer-testis antigen MAGE-C2 binds Rbx1 and inhibits ubiquitin ligase-mediated turnover of cyclin E. Oncotarget, 6(39), 42028-42039.

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Western Blot Antibody Specifications

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For western blot analysis, antibodies directed against the following proteins were used: GFP (SC-9996, Santa Cruz, 1:2000), α-tubulin (T6074, Sigma, 1:10000), α-tubulin (Ab18251, Abcam, 1:10000), GAPDH (60004-1lg, Proteintech, 1:20000), HA (16B12, Covance, 1:1000), GIGYF2 (NBP2-12812, Novusbio, 1:3000), FLAG-tag (F1804, Sigma, 1:500), DDX6 (A300-461A, Bethyl Laboratories, 1:1000) and GIGYF1 (A304-132A-M, Bethyl Laboratories, 1:500). Secondary antibodies were: IRDye 800CW goat anti-rabbit (LICOR, 1:10000), IRDye 680RD goat anti-rabbit (LICOR, 1:10000), IRDye 800CW goat anti-mouse (LICOR, 1:10000) and Alexa 680 goat anti-mouse (A21057, Life Technologies 1:10000). For Immunoprecipitation: HA (Covance), GIGYF2 (Novusbio) and mouse or rabbit IgG (Sigma). For Immunofluorescence: GIGYF2 (NBP2-12812, Novusbio, 1:500), HA (16B12, Covance, 1:1000), anti-mouse Alexa 488 (A28175, Invitrogen, 1:1000) and anti-rabbit Alexa 633 (A21070, Invitrogen, 1:1000).

Amaya Ramirez C.C., Hubbe P., Mandel N, & Béthune J. (2018). 4EHP-independent repression of endogenous mRNAs by the RNA-binding protein GIGYF2. Nucleic Acids Research, 46(11), 5792-5808.

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