Trypsinized cells and homogenized tissue samples were incubated for 15 min in 0.075 M KCl, fixed with methanol and acetic acid (3:1), and then slides were prepared using standard methods. FISH analyses were performed using fixed metaphase or interphase spreads of each cell hybrid using digoxigenin-labelled (Roche, Basel, Switzerland) DNA [human COT-1 DNA/mouse COT-1 DNA (Invitrogen), mouse minor satellite DNA and IGK-BAC (CH17-405H5 and CH17-216K2)] and biotin-labelled DNA [human COT-1 DNA/mouse COT-1 DNA, IGK-BAC (CH17-140P2), IGH-BAC (CH17-262H11, CH17-212P11 and RP11-731F5) and each part of the targeting vector], essentially as described previously10 (link). Chromosomal DNA was counterstained with DAPI (Sigma-Aldrich). Images were captured using an AxioImagerZ2 fluorescence microscope (Carl Zeiss GmbH, Jena, Germany).
Human cot 1 dna mouse cot 1 dna
Manufactured by Thermo Fisher Scientific
Sourced in Switzerland
Human COT-1 DNA/mouse COT-1 DNA is a laboratory reagent used in molecular biology applications. It is composed of purified repetitive DNA sequences found in the genomes of humans and mice. This product can be used in various DNA-based experiments and analyses, but a detailed description of its intended use would require more specific information.
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Lab products found in correlation
2 protocols using human cot 1 dna mouse cot 1 dna
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Fluorescence In Situ Hybridization Analysis
2
Fluorescence In Situ Hybridization Analysis
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The trypsinized cells and homogenized tissue samples were incubated for 15 min in 0.075 M KCl, fixed with methanol and acetic acid (3:1), and then slides were prepared using standard methods. FISH analyses were performed using fixed metaphase or interphase spreads of each cell hybrid using digoxigenin-labeled (Roche) DNA [human COT-1 DNA/mouse COT-1 DNA (Invitrogen)] and biotin-labeled DNA [UGT2-BAC (RP11-643N16), CYP3A-BAC (RP11-757A13), mouse COT-1 DNA, PGK-neo, β-actin-hisD, PGK-puro, and PGK-hygro], essentially as described previously (12 (link)). Chromosomal DNA was counterstained with DAPI (Sigma-Aldrich). Images were captured using an AxioImagerZ2 fluorescence microscope (Carl Zeiss).
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