C57bl 6 wt mice

Manufactured by Charles River Laboratories

Sourced in China, Canada, United States, Germany, United Kingdom, France

The C57BL/6 WT mice are a widely used inbred strain of laboratory mice. They are considered the wild-type (WT) strain, serving as a control or reference strain in various research studies.

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C57BL/6 mice (3627 citations) C57BL/6 (788 citations) C57BL/6 WT (24 citations) WT mice (21 citations) C57BL/6 WT female mice (7 citations) Black C57BL/6 WT mice (3 citations) WT C57BL/6 male mice (2 citations) C57BL/6-Ly5.1 WT mice (2 citations) WT NCI C57BL/6 mice (2 citations) C57BL/6 (B6) WT mice (2 citations)

153 protocols using c57bl 6 wt mice

Transgenic Mouse Models in Neuroscience

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All experimental protocols were approved by the Institutional Ethics Committee and followed the guidelines for Animal Use in Research and Education at Wenzhou Medical University, China (NO. wydw2021-0563). C57BL/6 WT mice were purchased from institute-approved vendors (Beijing Vital River Laboratory Animal Technology Co., Ltd.).
vGluT2-IRES-CRE mice JAX Strain (016963)^{57 (link)} and Nt5e (CD73) KO (CD73-KO) mice (JAX Strain 018986)^{58 (link)} were obtained from Jackson Laboratory. ENT1-KO mice, ENT2-KO mice,^{36 (link)} and GRAB_ATP1.0 knock-in (GRAB_ATP1.0-KI) mice were generated using CRISPR/Cas9 technology by Beijing Biocytogen and kindly provided by Dr. Yulong Li’s laboratory.^{36 (link)} The mice were housed in an enriched environment with ad libitum access to food and water under a 12-h light/12-h dark cycle. Mice with implants for EEG/EMG recording, optogenetic manipulation, and fiber photometry recording were housed individually.

Zhou X., He Y., Xu T., Wu Z., Guo W., Xu X., Liu Y., Zhang Y., Shang H., Huang L., Yao Z., Li Z., Su L., Li Z., Feng T., Zhang S., Monteiro O., Cunha R.A., Huang Z.L., Zhang K., Li Y., Cai X., Qu J, & Chen J.F. (2024). 40 Hz light flickering promotes sleep through cortical adenosine signaling. Cell Research, 34(3), 214-231.

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Mice for Fgl2 Knockout Study

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C57BL/6 WT mice were purchased from Beijing Vital River Laboratory Animal Technology, Co, Ltd (Beijing, China). Fgl2^-/- mice on a C57BL/6J background were constructed by Shanghai Model Organisms Center, Inc (Shanghai, China). Female WT littermates between 6 and 8 weeks old were used as controls. Mice were maintained in a specific pathogen-free environment at the Animal Experiment Center of Tongji Hospital, following the procedures approved by the Tongji Hospital Animal Ethics Committee (TJH-201802001).

Li X., Gao Q., Wu W., Hai S., Hu J., You J., Huang D., Wang H., Wu D., Han M., Xi D., Yan W., Chen T., Luo X., Ning Q, & Wang X. (2022). FGL2–MCOLN3-Autophagy Axis–Triggered Neutrophil Extracellular Traps Exacerbate Liver Injury in Fulminant Viral Hepatitis. Cellular and Molecular Gastroenterology and Hepatology, 14(5), 1077-1101.

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Evaluating ZIKV Vaccine Efficacy in Mice

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Specific-pathogen-free (SPF) C57BL/6 WT mice were purchased from Beijing Vital River Laboratory Animal Technology (licensed by Charles River). Six- to eight-week-old male and female C57BL/6 mice (4 for each group) were immunized with 2 × 10³ FFU, 2 × 10⁵ FFU, 2 × 10⁷ FFU CYV-ZIKV or PBS through the i.p. injection route. Two weeks later, the mice were boosted through the same route and immunization dose. After immunization, a weight change was detected in the mice. At 1, 3, and 4 months after i.p. injection, mice were bled to measure neutralizing antibody titers using a FRNT50 assay.

Wen D., Ding L.S., Zhang Y., Li X., Zhang X., Yuan F., Zhao T, & Zheng A. (2022). Suppression of flavivirus transmission from animal hosts to mosquitoes with a mosquito-delivered vaccine. Nature Communications, 13, 7780.

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Analyzing iNKT Cell Activation in Tumors

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C57BL/6 WT mice were purchased from Beijing Vital River Laboratory Animal Technology (Beijing, China). Vα14 Tg. Cxcr6^gfp/+ mice on the C57BL/6 background were provided by Dr. Albert Bendelac (The University of Chicago).^{22 (link)} All mice were bred in a specific pathogen-free facility at the University of Science and Technology of China. All animal experiments were approved by the Institutional Animal Care and Use Committee of the University of Science and Technology of China.
Indicated lipid antigens (2 μg per mouse) were intraperitoneally injected into WT, Il4ra^−/−, or MC38 tumor-bearing mice. To measure DC activation and cytokine production in the serum, tissue, and serum samples were collected after 8 h. Cytokines produced by intratumoral iNKT cells were measured with cytokine secretion Assay-Detection Kit (Miltenyi Biotec).

Wang L., Liu Z., Wang L., Wu Q., Li X., Xie D., Zhang H., Zhang Y., Gu L., Xue Y., Yue T., Liu G., Ji W., Wei H., Xu T, & Bai L. (2019). Spatial distribution of IL4 controls iNKT cell-DC crosstalk in tumors. Cellular and Molecular Immunology, 17(5), 496-506.

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Establishment of OCT3 Knockout Mice

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Eight-week-old male C57BL/6 (WT) mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. After arrival, the WT mice were raised for 2 wk without any treatment to adapt to the environment and avoid stress.

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mice were originally generated by Zwart et al.^{35 (link)} and kindly gifted from K. Tieu (Florida International University). The animals were housed at

23 \pm 1 ° C

55 \pm 5 %

humidity with a 12‐h light/dark cycle (lights on between 0700 and 1900 hours). Water and standard chow were provided ad libitum.

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mice were bred in an animal platform, at the Institute of Neurology, Huashan Hospital, Fudan University. Eight- to twelve-wk-old homozygous

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breeders were mated, and once the vaginal embolus was found, the pregnant female mice were raised separately from other mice, until they gave birth to the newborns and fed for 4 wk. Mouse tail tissue was taken for genotyping by quantitative polymerase chain reaction (qPCR). After genotyping, male

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mice were used for experiments. The study was performed with the approval of the Institutional Animal Care and Use Committee of Fudan University. The experiments were performed according to the National Institute of Health Guide for the Care and Use of Laboratory Animals.³⁶

Han S., Feng Y., Guo M., Hao Y., Sun J., Zhao Y., Dong Q., Zhao Y, & Cui M. (2022). Role of OCT3 and DRP1 in the Transport of Paraquat in Astrocytes: A Mouse Study. Environmental Health Perspectives, 130(5), 057004.

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Fungal Infection in Card9-KO Mice

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Card9-KO mice (C57BL/6 background) were generously provided by Xin Lin (Tsinghua University School of Medicine, Beijing, China, and MD Anderson Cancer Center, Houston, TX). In this study, 6−8-week-old Card9-KO and C57BL/6 WT mice (Vital River Laboratories, Beijing, China) were maintained in specific pathogen-free facilities at the Institute of Clinical Pharmacology of Peking University. The WT and Card9-KO mice were injected at two hind footpads subcutaneously with 100 μL viable P. expanda (1×10⁹ particles/mL). Skin biopsy specimens were obtained, and slides were stained with periodic acid-Schiff for histopathological analysis.

Huang C., Deng W., Zhang Y., Zhang K., Ma Y., Song Y., Wan Z., Wang X, & Li R. (2022). CARD9 deficiency predisposing chromoblastomycosis: A case report and comparative transcriptome study. Frontiers in Immunology, 13, 984093.

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Acute Lung Injury Mouse Model

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A total of 40 C57BL/6 WT mice purchased from Vital River (Beijing, China) were anesthetized and underwent oral intubation. Next, mouse models of acute lung injury were established by intratracheal instillation of LPS (1.5 mg/kg). The healthy control mice were injected with NaCl solution via the tail vein. The lentiviruses of agomiR-150-5p-NC, agomiR-150-5p (20 mg/kg; Sigma-Aldrich Chemical Company, St Louis MO, USA) and sh-MALAT1 NC, sh-MALAT1 (10 mg/kg; GenePharma, Shanghai, China) were intratracheally injected into the mice (n = 8 for each treatment). One day after injection, the mice were stimulated by LPS. On the 21^st day after LPS stimulation, the mice were euthanized and their lung tissues were collected.

Yao M.Y., Zhang W.H., Ma W.T., Liu Q.H., Xing L.H, & Zhao G.F. (2020). Long non-coding RNA MALAT1 exacerbates acute respiratory distress syndrome by upregulating ICAM-1 expression via microRNA-150-5p downregulation. Aging (Albany NY), 12(8), 6570-6585.

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Lgr5-eGFP-IRES-creERT2 Mouse Model

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Lgr5‐eGFP‐IRES‐creERT2 mice (Cyagen Biosciences Inc.) and C57BL/6 WT mice (Beijing Vital River Laboratory Animal Technology Co., Ltd.) were used for the experiments. All the animal experiments in this study were approved by the Ethics Committee of the Health Science Center of Xi'an Jiaotong University. Mice were housed in plastic cages and were maintained on a 12‐h light–dark cycle at room temperature (22–26°C) with sterile pellet food and water ad libitum.

Zhang L., He Y., Dong L., Liu C., Su L., Guo R., Luo Q., Gan B., Cao F., Wang Y., Song H, & Li X. (2023). Perturbation of intestinal stem cell homeostasis and radiation enteritis recovery via dietary titanium dioxide nanoparticles. Cell Proliferation, 56(8), e13427.

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Characterization of CD11b and E4BP4 Knockout Mice

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CD11b^−/− mice (Itgam^tm1Rws) were purchased from Jackson Laboratory (Bar Harbor, ME, USA), E4BP4^−/− mice (32 (link)) were provided by Professor H. Brady. Both strains have been backcrossed onto C57BL/6 strain for at least 10 generations. C57BL/6 WT mice were purchased from Vital River Laboratories (Beijing, China). Male mice (6–8 weeks old) were used in all experiments; all mice were maintained in specific pathogen-free conditions. The Ethics Review Committee for Animal Experimentation at Xi’an Jiaotong University approved and oversaw all mouse experiments.

Liu C.F., Min X.Y., Wang N., Wang J.X., Ma N., Dong X., Zhang B., Wu W., Li Z.F., Zhou W, & Li K. (2017). Complement Receptor 3 Has Negative Impact on Tumor Surveillance through Suppression of Natural Killer Cell Function. Frontiers in Immunology, 8, 1602.

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Standardized Mouse Experimental Protocols

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The animal protocols were approved by the Laboratory Animal Welfare and Ethics Committee and adhered to the Animal Ethics Statement (Approved No. AMUWEC20210143) (Army Medical University). C57BL/6 WT mice were purchased from Vital River Laboratories (Beijing, China). Fxr^−/− mice with a C57BL/6 background were obtained from the Jackson Laboratory (Cat# 007214, Bar Harbor, USA). Prof. W.H. from the Department of Laboratory Animal Science (Army Medical University) provided the GF mice. Male mice weighing 20–22 g and aged 6–8 weeks at the beginning of the study were used. Mice were bred under a specific pathogen-free (SPF) environment with controlled conditions (12-h light/dark cycle at 21 ± 1 °C and 45 ± 5% humidity). Throughout the study, mice were given ad libitum access to food and water. Across all experimental conditions, mice were all of the same species, sex, age and rearing conditions (at Army Medical University) and were strictly randomized into groups.

Lei Y., Tang L., Chen Q., Wu L., He W., Tu D., Wang S., Chen Y., Liu S., Xie Z., Wei H., Yang S, & Tang B. (2022). Disulfiram ameliorates nonalcoholic steatohepatitis by modulating the gut microbiota and bile acid metabolism. Nature Communications, 13, 6862.

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