Rat tnf alpha elisa kit

Manufactured by RayBiotech

Sourced in United States

The Rat TNF-alpha ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of rat tumor necrosis factor-alpha (TNF-alpha) levels in cell culture supernatants, plasma, and serum samples.

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Lab products found in correlation

Rat il 10 platinum elisa, by Thermo Fisher Scientific (1 mentions) Eontm microplate spectrophotometer, by Agilent Technologies (1 mentions) Celltiter glo 2.0 assay kit, by Promega (1 mentions) Anti actin, by Merck Group (1 mentions) Anti tnf α, by Thermo Fisher Scientific (1 mentions) Anti cyclin d3, by BD (1 mentions) Spectramax abs microplate reader, by Molecular Devices (1 mentions) Span 80, by Maclin Biochemical Technology (1 mentions) Tetrahydrofuran, by Tianjin Damao (1 mentions) Liquid paraffin, by Maclin Biochemical Technology (1 mentions) Il 1β elisa kit, by R&D Systems (1 mentions)

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Rat ELISA kit (7 citations) ELISAs (7 citations) TNF-alpha (3 citations)

6 protocols using rat tnf alpha elisa kit

Inflammatory Cytokine Profiling in Ileal Mucosa

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The concentration of cytokines IL-6, TNF-a, IL-10 and NF-kB, the marker of inflammation, was measured in the homogenate of ileal mucosa by ELISA. Intestinal mucosa was prepared as previously described by Doligalska et al. [15] . The concentration of cytokines and NF-kB were determined by commercially available kits Rat IL-6
Platinum ELISA (eBioscience, USA), Rat IL-10 Platinum ELISA (eBioscience, USA), Rat TNF-alpha ELISA kit (RayBiotech, Inc., USA) a Rat NF-kB p65 ELISA kit (Elabscience Biotechnology, China) according to the manufacturer0s instruc-tions. The plates were read at 450 nm using EonTM Microplate Spectrophotometer (Bio-Tek Instruments, USA). The final concentration was expressed as ng/g of wet mucosa

Štofilová J., Langerholc T., Botta C., Treven P., Gradišnik L., Salaj R., Šoltésová A., Bertková I., Hertelyová Z, & Bomba A. (2017). Cytokine production in vitro and in rat model of colitis in response to Lactobacillus plantarum LS/07. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 94.

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Viability and Immunoblot Assay Protocol

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For the viability assay, the CellTiter-Glo 2.0 Assay kit was purchased from Promega (Cat #G9243, Madison, WI). For immunoblotting, the following antibodies were purchased: anti-rat NF-κB p65 (dilution– 1:750, Thermo Fisher, Cat #436700, Waltham, MA), anti-TNF-α (dilution– 1:1000, Thermo Fisher, Cat #BMS175, Waltham, MA), anti-AKAP95 (dilution– 1:2500, gifted by Vince Coghlan, OHSU, Portland, OR), anti-cyclin D3 (dilution– 1:1000, BD Biosciences, Cat #610279, Franklin Lakes, NJ), and anti-actin (dilution– 1:1000, Sigma-Aldrich, Cat #A2066, St. Louis, MO). For TNF ELISA, the Rat TNF-alpha ELISA kit was purchased from RayBiotech (Cat #ELR-TNFa-1, Norcross, GA).

Henry C., Wilcox M, & Asirvatham A.L. (2024). Forskolin-mediated cAMP activation upregulates TNF-α expression despite NF-κB downregulation in LPS-treated Schwann cells. PLOS ONE, 19(4), e0302223.

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Forskolin Modulates TNF-alpha Secretion

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Media samples were collected from the 6-well plates (as described above), and the Rat TNF-alpha ELISA kit (RayBiotech, Cat #ELR-TNFa-1, Norcross, GA) was used to quantify the amount of TNF-α secreted by the RT4-D6P2T cells in response to the following treatments: 0.1, 1, or 10 μg/mL of LPS, in N₂ media (control) or N₂ media supplemented with 2 μM of forskolin, for 3 hours. The samples were diluted two-fold, and the ELISA was conducted following the manufacturer’s protocol. Immediately after the final incubation, the 96-well plate was read at 450 nm in the SpectraMax ABS Microplate Reader (Molecular Devices, San Jose, CA). TNF-α levels were calculated based on the standard curve, and the data are presented as mean fold change (over the N₂ control [no LPS]) to show the effects of the forskolin treatment on TNF-α secretion by cells treated with different doses of LPS.
doi: https://doi.org/10.17504/protocols.io.3byl4qp88vo5/v1.

Henry C., Wilcox M, & Asirvatham A.L. (2024). Forskolin-mediated cAMP activation upregulates TNF-α expression despite NF-κB downregulation in LPS-treated Schwann cells. PLOS ONE, 19(4), e0302223.

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Topical Triptolide-Based Anti-Inflammatory Formulation

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Triptolide (L-004-181225, Chengdu Ruifinsi Biological Co., Ltd., Chengdu, China), soybean phospholipids (Tixie Shanghai Chemical Industrial Development Co., Ltd., Shanghai, China), and tetrahydrofuran (Tianjin Damao Chemical Reagent Factory, Tianjin, China) were analytically pure. Ethyl Nipagin (Shanghai Macklin Biochemical Co., Ltd., Shanghai, China), beeswax (Shanghai Macklin Biochemical Co., Ltd., Shanghai, China), glyceryl monostearate (Shanghai Macklin Biochemical Co., Ltd., Shanghai, China), white petroleum jelly (Shanghai Aladdin Biochemical Technology Co., Ltd., Shanghai, China), liquid paraffin (Shanghai Macklin Biochemical Co., Ltd., Shanghai, China), solid paraffin (Shanghai Macklin Biochemical Co., Ltd., Shanghai, China); Span 80 (Shanghai Macklin Biochemical Co., Ltd., Shanghai, China), polysorbate 80 (Hunan Er-Kang Pharmaceutical Co., Ltd., Hunan, China), BCG vaccine (Sinopharm Chemical Reagent Co. Ltd., Shanghai, China), rat TNF-alpha ELISA Kit (RayBiotech Life, Inc., Norcross, GA), rat IL-1β ELISA Kit and rat IL-6 ELISA Kit (Ranjeck Technology Co., Ltd., Hefei, China), methanol, and ethanol were of chromatographic pure grade.

Liu X.Y., Pei W.J., Wu Y.Z., Ren F.L., Yang S.Y, & Wang X. (2021). Transdermal delivery of triptolide–phospholipid complex to treat rheumatoid arthritis. Drug Delivery, 28(1), 2127-2136.

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Liver Tissue Biomarker Quantification

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Liver tissue TNF-α was measured by using Rat TNF-alpha ELISA Kit supplied by RayBiotech (Inc., Norcross, Georgia, USA, CAT. No. ELR-TNFa), TGF-β1 was measured by using Rat TGF-β1 PicoKine ELISA Kits (Pleasanton, USA, CAT. No. MBS824788). The level of FFA in liver tissue was determined by using Rat FFA ELISA Kit (BioSource International, Inc., California, USA, Cat. No: MBS014345).

Lebda F.M., El Agaty S.M., Ali R.H., Galal Hamam G., Abd El-Monem A.M, & Lasheen N.N. (2022). Cerium oxide nanoparticles modulate liver X receptor and short heterodimer partner, and attenuate liver steatosis and steatohepatitis in a rat model of postmenopausal obesity. General physiology and biophysics, 41(5).

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Quantifying TNF-α and IL-1β in Kidney

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The protein levels of TNF-α and IL-1β were measured in the kidney homogenate using Rat TNF- alpha ELISA kit (RayBiotech, Inc., Norcross, Georgia, USA) and IL-1β ELISA Kit (R&D System, Minneapolis, MN, USA), respectively, according to manufacturer's instructions.

Attia S.H., Elshazly S.M., Abdelaal M.M, & Soliman E. (2022). Reno-protective effect of mangiferin against methotrexate-induced kidney damage in male rats: PPARγ-mediated antioxidant activity. Saudi Pharmaceutical Journal : SPJ, 30(9), 1252-1261.

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