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Ava500 spectrometer

Manufactured by Bruker

The Bruker AVA500 is a high-performance Nuclear Magnetic Resonance (NMR) spectrometer. It is designed to perform precise and accurate measurements of chemical and physical properties of materials. The AVA500 utilizes a strong magnetic field and radio frequency pulses to analyze the atomic-level structure and dynamics of samples. It is a versatile instrument suitable for a wide range of applications in fields such as chemistry, materials science, and biological research.

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3 protocols using ava500 spectrometer

1

NMR Characterization of Monomers, Polymers, and RAFT Agents

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All monomers, polymers, and RAFT agents were analyzed on a Bruker AVA500 spectrometer in CDCl3 or D2O at either 500 MHz (H1 NMR) or 125 MHz (C13).
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2

Characterization of Organic Compounds

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Unless otherwise stated, all solvents and reagents were purchased from Sigma-Aldrich, Fisher Scientific UK, Alfa Aesar (Heysham, UK), Acros Organics (Geel, Belgium), or VWR International (Lutterworth, UK) and used without further purification. Deionized water was produced using a Milli-Q purification system. 1H and 13C NMR Spectra were recorded on a Bruker AVA500 spectrometer operating at 500.12 and 125.76 MHz for 1H and 13C, respectively. 71Ga NMR spectra were recorded on a Bruker PRO500 spectrometer at 152.55 MHz.
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3

Biomaterial-based 3D Cell Culture Conditions

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Dulbecco Modified Eagle Medium, alpha-MEM, penicillin/ streptomycin, DPBS were obtained from Lonza. Ascorbic acid 2-phosphate and glycerol 2-phosphate disodium salt hydrate were purchased from Sigma. Fetal bovine serum and M199 medium was obtained from Gibco. ECGS/H were from Promocell. VEGF, TGFb 3 were from Peprotech. Poly(lactide-co-glycolide) (PLGA) (50 : 50 Resomer RG 502, RG 505, RG 504, and 85 : 15 PLGA ester terminated M w 50 000-75 000) and tween 20, biotin, chitosan (CS) (from crab shells), polyethylenimine (PEI) (average M w = 800 Da), poly(L-lactic acid) (PLLA) (viscosity B1.0 dL g À1 ) and poly(vinyl acetate) (PVAc) (average M w = 140 kDa), palmitic acid N-hydroxysuccinimide ester (NHS-palmitic acid) and avidin were from Sigma. All other reagents were from Sigma and used without further purification. Analytical grade chloroform, glacial acetic acid, dichloromethane (DCM) were from Thermo Fisher Scientific, UK. Emulsions were generated using a Branson sonifier 250 at 40% max amplification. 1 H nuclear magnetic resonance spectra were recorded on a Bruker AVA500 spectrometer (500 MHz respectively) at 298 K in deuterated solvents.
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