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α-inhibin is a glycoprotein hormone produced primarily by the gonads. It functions to inhibit the secretion of follicle-stimulating hormone (FSH) from the anterior pituitary gland.

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Lab products found in correlation

Desmin, by Gene Tech (2 mentions) Calretinin, by ZSGB-BIO (2 mentions) Automatic immunostaining device, by Leica (2 mentions)

2 protocols using α inhibin

1

Immunohistochemical Profiling of Tumor Samples

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Collected tumours or normal tissue after removal of paraffin as the experimental subject. Consecutive 4-μm thick unstained sections for immunohistochemical staining, which was performed using the Leica automatic immunostaining device (Leica Microsystems, Inc.). Primary antibodies against CD10 (1:100; no. 563871; DAKO; DK), α-inhibin (1:100; no. GT230202; CHN), SMA (1:50; no. MAB-0980; MXB; CHN), desmin (1:300; no. GT225202; Gene tech; CHN), TFE3 (1:100; no. ZA-0657; ZSGB-BIO; CHN), calretinin (1:100; no. ZM-0063; ZSGB-BIO; CHN), WT-1 (1:100; no. ZM-0269; ZSGB-BIO; CHN), and EMA (1:300; no. GM061302; Gene tech; CHN). Appropriate positive and negative controls were simultaneously stained to validate the staining method.
Zhao L., Zhou Y., Liu Y., Luo Q., Jiang Q., Wang H, & Wang N. (2023). TFE3 is a Novel Biomarker of Ovarian Sclerosing Stromal Tumours. Research Square.
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2

Immunohistochemical Staining of Tumor Samples

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As the experimental subjects, tumors or normal tissues were collected after paraffin removal. Consecutive 4-μm-thick unstained sections were used for immunohistochemical staining, which was performed using the Leica automatic immunostaining device (Leica Microsystems, Inc.). Primary antibodies against CD10 (1:100; no. 563871; DAKO; DK), α-inhibin (1:100; no. GT230202; CHN), SMA (1:50; no. MAB-0980; MXB; CHN), desmin (1:300; no. GT225202; Gene tech; CHN), TFE3 (1:100; no. ZA-0657; ZSGB-BIO; CHN), calretinin (1:100; no. ZM-0063; ZSGB-BIO; CHN), WT-1 (1:100; no. ZM-0269; ZSGB-BIO; CHN), and EMA (1:300; no. GM061302; Gene tech; CHN). Appropriate positive and negative controls were simultaneously stained to validate the staining method.
Zhao L., Yang Z., Zhou Y., Liu Y., Luo Q., Jiang Q., Wang H, & Wang N. (2023). TFE3 nuclear expression as a novel biomarker of ovarian sclerosing stromal tumors and associated with its histological morphology. Journal of Ovarian Research, 16, 152.
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