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Antibodygoat anti rabbit or goat anti mouse igg hrp

Manufactured by Thermo Fisher Scientific
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The AntibodyGoat Anti-Rabbit or Goat Anti-Mouse IgG-HRP is a laboratory reagent used as a secondary antibody in immunoassays and immunohistochemistry applications. It is a conjugate of goat-derived antibodies specific to rabbit or mouse immunoglobulin G (IgG) and the enzyme horseradish peroxidase (HRP), which can be used to detect and visualize target proteins or antigens.

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Lab products found in correlation

Ab76024, by Abcam (2 mentions) Ab109235, by Cell Signaling Technology (2 mentions) Vinculin, by Merck Group (2 mentions) Cell lysis buffer, by Cell Signaling Technology (2 mentions) Laemmli sample buffer, by Bio-Rad (2 mentions) Criteriontris glycine extended gels, by Bio-Rad (2 mentions) Azure c600, by Azure Biosystems (2 mentions) Image studio lite software version 5, by LI COR (2 mentions) Trans blot turbo system, by Bio-Rad (2 mentions)

Close spelling variants of this product

Goat anti-rabbit HRP Goat anti-mouse HRP HRP-anti-mouse Mouse anti-IgG IgG Rabbit

2 protocols using antibodygoat anti rabbit or goat anti mouse igg hrp

1

Western Blot Analysis of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
1*106 cells were lysed (cell lysis buffer, Cell signaling
technology) and denatured in 1x Laemmli sample buffer (Bio-rad) containing
2-mercaptoethanol (Sigma Aldrich). Proteins were separated on Criterion
Tris-Glycine eXtended gels (Bio-rad), transferred to PVDF membranes using the
Trans-Blot Turbo system (Bio-rad), incubated with primary antibodies targeting
Catalase (Cell Signaling #14097 & Abcam #ab76024), xanthine oxidase
(Abcam #ab109235), Bcl-2 (Cell Signaling #2870), and with secondary antibody
Goat Anti-Rabbit or Goat Anti-Mouse IgG-HRP (Thermo Fischer). Proteins were
visualized using chemiluminescence on an Azure C600 (Azure Biosystems).
Quantification was performed using LI-COR Image Studio Lite software version
5.2. Vinculin (Sigma Aldrich), tubulin or beta actin (Sigma Aldrich) was used to
normalize for protein input.
Kampen K.R., Sulima S.O., Verbelen B., Girardi T., Vereecke S., Rinaldi G., Verbeeck J., de Beeck J.O., Uyttebroeck A., Meijerink J.P., Moorman A.V., Harrison C.J., Spincemaille P., Cools J., Cassiman D., Fendt S.M., Vermeersch P, & De Keersmaecker K. (2018). The ribosomal RPL10 R98S mutation drives IRES-dependent BCL-2 translation in T-ALL. Leukemia, 33(2), 319-332.
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2

Western Blot Analysis of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
1*106 cells were lysed (cell lysis buffer, Cell signaling
technology) and denatured in 1x Laemmli sample buffer (Bio-rad) containing
2-mercaptoethanol (Sigma Aldrich). Proteins were separated on Criterion
Tris-Glycine eXtended gels (Bio-rad), transferred to PVDF membranes using the
Trans-Blot Turbo system (Bio-rad), incubated with primary antibodies targeting
Catalase (Cell Signaling #14097 & Abcam #ab76024), xanthine oxidase
(Abcam #ab109235), Bcl-2 (Cell Signaling #2870), and with secondary antibody
Goat Anti-Rabbit or Goat Anti-Mouse IgG-HRP (Thermo Fischer). Proteins were
visualized using chemiluminescence on an Azure C600 (Azure Biosystems).
Quantification was performed using LI-COR Image Studio Lite software version
5.2. Vinculin (Sigma Aldrich), tubulin or beta actin (Sigma Aldrich) was used to
normalize for protein input.
Kampen K.R., Sulima S.O., Verbelen B., Girardi T., Vereecke S., Rinaldi G., Verbeeck J., de Beeck J.O., Uyttebroeck A., Meijerink J.P., Moorman A.V., Harrison C.J., Spincemaille P., Cools J., Cassiman D., Fendt S.M., Vermeersch P, & De Keersmaecker K. (2018). The ribosomal RPL10 R98S mutation drives IRES-dependent BCL-2 translation in T-ALL. Leukemia, 33(2), 319-332.
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