Hydrogen peroxide h2o2

All chemicals were analytical grade and used as received. All solutions were prepared in Milli-Q deionized water from a Milli Q gradient A10 water purification system (Molsheim, France). CTZ bulk powder (Cetirizine dihydrochloride, Ph. Eur) was purchased from Jubilant Lifesciences Ltd (Mysore, India) and kindly provided by Reig Jofre Group (Barcelona, Spain). HPLC grade acetonitrile was obtained from Panreac (Barcelona, Spain). Ortho-phosphoric acid 85% was purchased from Panreac (Barcelona, Spain). Potassium phosphate dibasic Ph. Eur. (K₂HPO₄) was purchased from Fagron (Terrassa, Spain). Hydrochloric acid 37%, sodium hydroxide and hydrogen peroxide (H₂O₂) at 33% were purchased from Panreac (Barcelona, Spain).

Cells were treated with one of three inhibitors, as indicated: 1 µM LY3009120 (Selleckchem), 1 µM Sorafenib (Santa Cruz Biotechnology) or 10 µM SB203580 (Selleckchem). Different cellular stress types were induced using different stressors: oxidative stress by 500 µM or 50 µM hydrogen peroxide (H₂O₂) (Panreac), depending on the experiment; saline stress by 100 mM NaCl.

Manganese sulfate monohydrate (MnSO₄.H₂O) and hydrogen peroxide (H₂O₂) were purchased from Panreac (Barcelona, Spain). Urea, ammonia solution, and potassium hydroxide (KOH) were obtained from Sigma-Aldrich. Deionized water (DIW) was used to prepare all aqueous solutions.