Antibodies: anti-HA tag (clone 3F10) (Roche Applied Science), anti-β-actin and anti-alpha-tubulin (Sigma-Aldrich), anti-Smac (Upstate Cell Signaling Solutions, Lake Placid, NY), anti-Hsp60 (Enzo Life Sciences, Plymouth Meeting, PA), anti-VDAC1 (Calbiochem, Darmstadt, Germany), anti-GAPDH, anti-PDK4 and anti-Vinculin (Santa Cruz), anti-PGC-1α (a gift from Dr. Daniel P. Kelly, Sanford-Burnham Medical Research Institute, FL), anti-pAMPK, anti-pCREB and anti-CREB (Cell Signaling Technologies, Boston, MA), anti-CHCHD4 (Protein-Tech Group, IL), anti-Tim23 (BD Biosciences, San Diego, CA). The peroxidase-conjugated goat anti-rat, goat anti-rabbit, goat anti-mouse and horse anti-goat antibodies were from Vector Laboratories (Burlingame, CA). Rabbit polyclonal antibodies specific for human CHTM1 were generated in our laboratory through ProSci Inc. (Poway, CA) using full-length recombinant human CHTM1 protein purified from Escherichia coli. For cell transfections, Mirus (Madison, WI) and Lipofectamine 2000 (Invitrogen, Carlsbad, CA) were used. Restriction endonucleases were from New England BioLabs (Ipswich, MA). PKA inhibitor-H89, p38 inhibitor-SB203580, PI3K inhibitor-LY294002 and PKC inhibitor-GO6983 were from Sigma-Aldrich (St. Louis, MO). Other chemical reagents were from Thermo Fisher Scientific and Sigma-Aldrich.
Pkc inhibitor go6983
Manufactured by Merck Group
PKC inhibitor-GO6983 is a laboratory reagent used to inhibit the activity of protein kinase C (PKC), a family of enzymes involved in various cellular processes. This compound acts as a potent and selective inhibitor of PKC, which can be useful in research applications where the regulation of PKC signaling is of interest. The core function of PKC inhibitor-GO6983 is to modulate PKC activity in a controlled experimental setting.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
P38 inhibitor sb203580, by Merck Group (2 mentions)
Restriction endonuclease, by New England Biolabs (2 mentions)
Anti pgc 1α, by Santa Cruz Biotechnology (2 mentions)
Anti tim23, by BD (2 mentions)
Pi3k inhibitor ly294002, by Merck Group (2 mentions)
Pka inhibitor h89, by Merck Group (2 mentions)
Anti chchd4, by Proteintech (2 mentions)
Anti ha tag clone 3f10, by Roche (2 mentions)
Anti β actin and anti alpha tubulin, by Merck Group (2 mentions)
Anti hsp60, by Enzo Life Sciences (2 mentions)
2 protocols using pkc inhibitor go6983
1
Antibody Reagents for Western Blot Analysis
2
Antibody Reagents for Western Blot Analysis
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Antibodies: anti-HA tag (clone 3F10) (Roche Applied Science), anti-β-actin and anti-alpha-tubulin (Sigma-Aldrich), anti-Smac (Upstate Cell Signaling Solutions, Lake Placid, NY), anti-Hsp60 (Enzo Life Sciences, Plymouth Meeting, PA), anti-VDAC1 (Calbiochem, Darmstadt, Germany), anti-GAPDH, anti-PDK4 and anti-Vinculin (Santa Cruz), anti-PGC-1α (a gift from Dr. Daniel P. Kelly, Sanford-Burnham Medical Research Institute, FL), anti-pAMPK, anti-pCREB and anti-CREB (Cell Signaling Technologies, Boston, MA), anti-CHCHD4 (Protein-Tech Group, IL), anti-Tim23 (BD Biosciences, San Diego, CA). The peroxidase-conjugated goat anti-rat, goat anti-rabbit, goat anti-mouse and horse anti-goat antibodies were from Vector Laboratories (Burlingame, CA). Rabbit polyclonal antibodies specific for human CHTM1 were generated in our laboratory through ProSci Inc. (Poway, CA) using full-length recombinant human CHTM1 protein purified from Escherichia coli. For cell transfections, Mirus (Madison, WI) and Lipofectamine 2000 (Invitrogen, Carlsbad, CA) were used. Restriction endonucleases were from New England BioLabs (Ipswich, MA). PKA inhibitor-H89, p38 inhibitor-SB203580, PI3K inhibitor-LY294002 and PKC inhibitor-GO6983 were from Sigma-Aldrich (St. Louis, MO). Other chemical reagents were from Thermo Fisher Scientific and Sigma-Aldrich.
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