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1

Antimicrobial Susceptibility Testing Protocol

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Antimicrobial susceptibility was determined by disk diffusion on Mueller-Hinton agar plates at 35°C and incubated for 16–18 h according to Clinical and Laboratory Standards Institute (CLSI) guidelines.13 Antibiotic discs included were piperacillin/tazobactam (TZP), ceftazidime, cefotaxime, meropenem, imipenem, ciprofloxacin, sulphamethoxazole/trimethoprim, gentamicin tetracycline and doxycycline (Oxoid, UK). The minimum inhibitory concentrations (MICs) for TZP, ceftazidime, cefotaxime, meropenem, imipenem, ciprofloxacin, sulphamethoxazole/trimethoprim, gentamicin tetracycline and doxycycline were determined by E-test (bioMérieux, Marcy-I´Etoile, France) following CLSI guidelines.
The MIC of colistin was determined by broth microdilution in cation-adjusted Mueller-Hinton broth according to CLSI guidelines.
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Antibiotic Susceptibility Testing for Gonorrhea

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Disk diffusion testing [12 ] was used to determine susceptibility to penicillin and tetracycline, and the Etest [13 ] was used to determine minimal inhibitory concentrations (MICs) of ciprofloxacin, cefixime, penicillin, tetracycline, azithromycin, and doxycycline (bioMerieux, France). Gonococcal strains ATCC 31426 and ATCC 49226 [12 ] and WHO F, WHO G, WHO L, WHO O, and WHO P [13 ] were used as references.
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Antibiotic Susceptibility of Animal Isolates

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Bacterial isolates recovered from animals that survived the experimental period were assessed for sensitivity to finafloxacin Biomerieux (Hampshire, United Kingdom, 0.002–32 μg/mL) and doxycycline (Biomerieux, Hampshire, United Kingdom 0.016–256 μg/mL) as per the manufacturer’s instructions.
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