Hybridizations of mRNAs and miRNAs were performed as previously reported by our group [22 (link)–24 (link)]. The oligo microarrays technology used for gene expression assays was the SurePrint G3 Human Gene Expression v3 8x60K oligo microarrays (G4851C, Agilent Technologies) and the Human miRNA Microarray Kit (V3) (8x15K-G4471A, Agilent Technologies) was used to assess the expression of miRNAs. For both microarrays, the total RNA amount (200 ng) and the one color (cyanine-3, Cy3) Quick Amp labeling kit (Agilent) was used. The hybridization steps varied according the manufacturer’ instructions, with 17 hours at 65∘C for mRNA and 24 hours at 55∘C for miRNA microarrays. Images were acquired using an Agilent DNA microarray scanner with SureScan technology (Agilent Technologies).
Sureprint g3 human gene expression v3 8x60k oligo microarrays
Manufactured by Agilent Technologies
The SurePrint G3 Human Gene Expression v3 8x60K oligo microarrays are designed for genome-wide gene expression analysis. The arrays contain approximately 60,000 probes covering well-characterized human genes and transcripts. The core function of these microarrays is to enable researchers to measure the expression levels of thousands of genes simultaneously in a single experiment.
Automatically generated - may contain errors
2 protocols using sureprint g3 human gene expression v3 8x60k oligo microarrays
1
mRNA and miRNA Expression Profiling
2
Microarray Analysis of mRNA and miRNA Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hybridizations of mRNAs and miRNAs were performed as previously reported by our group [22] (link)[23] (link)[24] (link). The oligo microarrays technology used for gene expression assays was the SurePrint G3 Human Gene Expression v3 8x60K oligo microarrays (G4851C, Agilent Technologies) and the Human miRNA Microarray Kit (V3) (8x15K-G4471A, Agilent Technologies) was used to assess the expression of miRNAs. For both microarrays, the total RNA amount (200 ng) and the one color (cyanine-3, Cy3) Quick Amp labeling kit (Agilent) was used. The hybridization steps varied according the manufacturer' instructions, with 17 hours at 65 • C for mRNA and 24 hours at 55 • CformiRNAmicroarrays. Images were acquired using an Agilent DNA microarray scanner with SureScan technology (Agilent Technologies).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!