Ft ir 420 spectrophotometer

The optical rotations were recorded on a JASCO P-2200 polarimeter. The UV spectra were recorded on a JASCO Ubest-55 spectrophotometer. The IR spectra were recorded on a JASCO FT/IR-420 spectrophotometer. The ECD spectra were recorded on a JASCO J-1500 spectropolarimeter. ¹H and ¹³C NMR spectra were recorded on a Bruker Avance II 600 MHz NMR spectrometer equipped with a cryoplatform using 3.0 mm micro cells (Shigemi Co., Ltd., Tokyo, Japan) for CD₃OD. The 3.35 ppm resonance of residual CD₂HOD in CD₃OD was used as the internal references for ¹H NMR spectra. The 49.8 ppm resonances of CD₃OD were used as the internal reference for the ¹³C NMR spectra. The MS spectra were recorded on a JEOL JMS-T100LP spectrometer. The flash column chromatography was performed with a Biotage Isolera flash purification system.

All the chemicals used for synthesis were of analytical grade. Metal salts were purchased from Merck. Dehydroacetic acid (DHA) was obtained from Aldrich. Ethanol, MEthanol, THF, and DMSO were used as it without further purification and were purchased from Sigma Aldrich. The progress of the reaction was monitored through TLC by using a combination of hexane-ethyl acetate (7:3) solvents. Melting points were determined on Gallonkamp melting point apparatus using open capillaries and were uncorrected. The solution conductivity measurements were performed by dissolving the complexes in DMSO with 10⁻³ M concentration and their molar conductivities were measured at room temperature. Infrared spectra were recorded on Jasco FT-IR-420 spectrophotometer. The multinuclear (¹H and ¹³C) NMR were recorded on Bruker AM-250 spectrometers in DMSO solution using TMS as an internal standard. UV-vis absorption spectra and DNA binding studies were recorded on UV-vis Spectrophotometer Shimadzu UV-1700. Mass spectra were recorded by using low mass MALDI standard mixture under 6000 m/z Ultra Flex Analysis placing 1 µL spot of DHB (2,4-dihydroxybenzoic acid) as matrix and 1 µL of the standard mixture in acetonitrile.