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Foxp3 clone 259d c7

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FoxP3 (clone 259D/C7) is a monoclonal antibody used for the detection and analysis of FoxP3-expressing cells, a transcription factor that is essential for the development and function of regulatory T cells. This antibody can be used in various immunological techniques, such as flow cytometry, immunohistochemistry, and western blotting, to identify and quantify FoxP3-positive cells.

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Lab products found in correlation

Pma ionomycin, by BD (2 mentions) Live dead fixable dead cell stain kit lda, by Thermo Fisher Scientific (2 mentions) Foxp3 buffer set, by BD (2 mentions) Facsaria flow cytometer, by BD (2 mentions) Brefeldin a, by BD (2 mentions)

Close spelling variants of this product

Anti Foxp3 (clone 259D/C7, Anti-Foxp3-PE (clone 259D/C7, PE-conjugated anti-Foxp3 (clone 259D/C7) Foxp3 Alexa Fluor 488 (clone 259D/C7, Foxp3 (259D/C7)

2 protocols using foxp3 clone 259d c7

1

Profiling Infant T-Cell Responses

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IFN-γ and TNF-α secreting, and FoxP3+, CD4+ T-cells in infant PBMC were identified by ICS (intracellular cytokine staining). PBMC were washed with media, and then left unstimulated or stimulated for 4 h with PMA/Ionomycin (BD Biosciences). The stimulations were done in the presence of 1 μl Brefeldin A (BD Biosciences). Cells were first stained with surface Ab to CD45RO (clone UCHL1), fixed and permeabilized with the FoxP3 buffer set (BD Biosciences), and then stained with Abs to CD3 (clone UCHT1), CD4 (clone SK3), CD8 (clone SK1), IFN-γ (clone B27), TNF-α (clone 6401.1111), and FoxP3 (clone 259D/C7) (all Abs from BD Biosciences). Cells were analyzed using a FACSAria flow cytometer (BD Biosciences). LIVE/DEAD® Fixable Dead Cell Stain Kit (LDA) (Invitrogen) was used to exclude nonviable cells from analysis. Relevant cells were identified as LDA−/CD3+/CD4+/CD8−/CD45RO+ or CD45RO−/IFN-γ+ or TNF-α+ or FoxP3+ cells (Supplementary Fig. 1). Data was analyzed using FlowJo® software (Treestar).
Libraty D.H., Zhang L., Woda M., Acosta L.P., Obcena A., Brion J.D, & Capeding R.Z. (2014). Neonatal BCG vaccination is associated with enhanced T-helper 1 immune responses to heterologous infant vaccines. Trials in vaccinology, 3, 1-5.
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2

Profiling Infant T-Cell Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols
IFN-γ and TNF-α secreting, and FoxP3+, CD4+ T-cells in infant PBMC were identified by ICS (intracellular cytokine staining). PBMC were washed with media, and then left unstimulated or stimulated for 4 h with PMA/Ionomycin (BD Biosciences). The stimulations were done in the presence of 1 μl Brefeldin A (BD Biosciences). Cells were first stained with surface Ab to CD45RO (clone UCHL1), fixed and permeabilized with the FoxP3 buffer set (BD Biosciences), and then stained with Abs to CD3 (clone UCHT1), CD4 (clone SK3), CD8 (clone SK1), IFN-γ (clone B27), TNF-α (clone 6401.1111), and FoxP3 (clone 259D/C7) (all Abs from BD Biosciences). Cells were analyzed using a FACSAria flow cytometer (BD Biosciences). LIVE/DEAD® Fixable Dead Cell Stain Kit (LDA) (Invitrogen) was used to exclude nonviable cells from analysis. Relevant cells were identified as LDA−/CD3+/CD4+/CD8−/CD45RO+ or CD45RO−/IFN-γ+ or TNF-α+ or FoxP3+ cells (Supplementary Fig. 1). Data was analyzed using FlowJo® software (Treestar).
Libraty D.H., Zhang L., Woda M., Acosta L.P., Obcena A., Brion J.D, & Capeding R.Z. (2014). Neonatal BCG vaccination is associated with enhanced T-helper 1 immune responses to heterologous infant vaccines. Trials in vaccinology, 3, 1-5.
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