Serum glucose, cholesterol, triglyceride (TG), and free fatty acids (FFAs) were analyzed using kits from Bioassay Systems (Hayward, CA, USA). To measure endotoxin clearance from serum, the LAL Chromogenic Endotoxin Quantitation Kit (Pierce Biotechnology, Rockford, IL, USA) was used. Serum alanine aminotransferase (ALT/GPT) was measured using a kit from Stanbio (Boerne, TX, USA). All the cytokines and chemokines were measured using the Luminex multiplex analysis system (Millipore, Billerica, MA, USA).
Luminex multiplex analysis system
Manufactured by Merck Group
Sourced in United States
The Luminex multiplex analysis system is a laboratory equipment designed for high-throughput, multiplexed bioassays. It utilizes color-coded magnetic microspheres, flow cytometry, and proprietary software to enable the simultaneous detection and quantification of multiple analytes in a single sample. The core function of the Luminex system is to facilitate the efficient and accurate analysis of complex biological samples.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using luminex multiplex analysis system
1
Metabolic Biomarker Analysis
2
Serum Glucose, Insulin, and Cytokine Analysis
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Serum glucose was analyzed using kits from Bioassay Systems (Hayward, CA, USA). Specific kits were used, following the manufacturer's instructions, to determine the concentrations of insulin (Shibayagi, Japan). Cytokine levels were measured using the Luminex multiplex analysis system (Millipore, Billerica, MA, USA).
3
Metabolite Profiling in Rodent Sera
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Blood samples were collected from each group of rat and mice. Different kits were used, following manufacturer instructions, to determine the concentrations of various metabolites in serum: insulin (Shibayagi, Shibukawa, Japan) and glucose (Shinyang, Seoul, South Korea). Serum IL-1β was measured using the Luminex multiplex analysis system (Millipore, Billerica, MA, USA).
4
Multiplex Cytokine and Metabolite Analysis
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Cytokine levels were measured utilizing the Luminex multiplex analysis system (Millipore). Kits from Bioassay Systems (Hayward, CA, USA) were used to analyze serum glucose levels. Insulin concentrations were measured using a specific kit (Shibayagi, Shibukawa, Japan).
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