Thioglycolic acid (TGA, C
2H
4O
2S, ≥99%),
cadmium acetate dihydrate (Cd(CH
3COO)
2·2H
2O, 99.5%),
(3-aminopropyl)triethoxysilane (APTES, H
2N(CH
2)
3Si(OC
2H
5)
3, 99%),
tetraethyl orthosilicate (TEOS, SiC
8H
20O
4, 98%), ammonia (NH
3, ≥99.9%), cetyltrimethylammonium bromide (CTAB, C
19H
42BrN, ≥98%), poly(ethylene glycol) BioUltra 3000, poly(ethylene glycol) BioUltra 6000, poly(ethylene glycol)
BioUltra 8000,
p-toluenesulfonyl chloride (CH
3C
6H
4SO
2Cl, ≥98%),
hydrochloric acid (HCl, 37%),
magnesium sulfate (MgSO
4, ≥99.5%),
sodium ascorbate, bovine serum albumin (BSA), lysozyme (LYS), carbonic anhydrase (CA), ovalbumin (OVA), hemoglobin (Hb), myoglobin (Myb), and
cytochrome c (CYT) were purchased from Sigma-Aldrich (Lisbon, Portugal).
Sodium hydroxide (NaOH, ≥98%) was produced by Panreac.
Sodium tellurite (Na
2TeO
3, 99.5%) and
ammonium nitrate (NH
4NO
3, 99.9%) were purchased from Alfa Aesar. Sodium borohydride (NaBH
4, 99%), ethylene glycol (C
2H
6O
2, ≥99.5%) and (3-isocyanatopropyl)triethoxysilane (IPTES, (C
2H
5O)
3Si(CH
2)
3NCO, 95%) were produced by Fluka. Methanol (MeOH), ethanol (EtOH), tetrahydrofuran (THF, C
4H
8O, ≥99.9%), and
chloroform (CHCl
3) were purchased by Carlo Erba Reagents. All the reagents and solvents were of analytical reagent grade and were used as received. All cell culture reagents were purchased from Gibco (Alfagene, Lisbon, Portugal) unless stated otherwise.
Marcelo G., Ariana-Machado J., Enea M., Carmo H., Rodríguez-González B., Luis Capelo J., Lodeiro C, & Oliveira E. (2018). Toxicological Evaluation of Luminescent Silica Nanoparticles as New Drug Nanocarriers in Different Cancer Cell Lines. Materials, 11(8), 1310.