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Ultrasoundgate cm16

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Sourced in Germany

The UltraSoundGate CM16 is a compact microphone designed for recording ultrasonic vocalizations. It features a built-in preamplifier and can be connected directly to a computer's USB port for data acquisition and analysis.

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Lab products found in correlation

Saslab pro, by Avisoft (3 mentions) Ultrasoundgate 416h, by Avisoft (2 mentions) Recorder software, by Avisoft (2 mentions) Giemsa solution, by Polysciences (1 mentions) Ultrasoundgate 116hb, by Avisoft (1 mentions)

8 protocols using ultrasoundgate cm16

1

Ultrasonic Vocalizations in Rodent Pups

Cited 1 time
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Ultrasonic vocalizations (USVs) were tested on PND3. Each pup was removed from the litter and placed in a sound-recording chamber for 5 min where USVs were recorded using a microphone (Avisoft UltraSoundGate CM16 condenser ultrasound microphone, Avisoft Bioacoustics, Glienicke, Germany). USV analysis was performed using the Avisoft Sound Analysis and Synthesis Software pro (Avisoft Bioacoustics) using the automatic detection settings, with each call manually confirmed in accordance with previous studies.32 (link) The number of USV calls made per minute over the 5-min separation period was determined. Where a call could not be differentiated from background noise, the animal was excluded from analysis.
Short A.K., Fennell K.A., Perreau V.M., Fox A., O'Bryan M.K., Kim J.H., Bredy T.W., Pang T.Y, & Hannan A.J. (2016). Elevated paternal glucocorticoid exposure alters the small noncoding RNA profile in sperm and modifies anxiety and depressive phenotypes in the offspring. Translational Psychiatry, 6(6), e837-.
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2

Analyzing Pup Ultrasonic Vocalizations

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Ultrasonic Vocalizations (USVs) emitted by pups after maternal separation for 3 min on post-natal day (PND) 4, 6, 8, 10 and 12 were analyzed using Avisoft Bioacoustics system as previously described [61 (link),62 (link)]. Briefly, USVs were recorded with ultrasound microphone (UltraSoundGate CM16, Avisoft Bioacoustics, Glienicke/Nordbahn, Germany) sensitive to frequencies of 10–180 kHz and analyzed with SasLab Pro (version 5.2; Avisoft Bioacoustics, Glienicke/Nordbahn, Germany). Number and duration of calls were analyzed.
Aria F., Bonini S.A., Cattaneo V., Premoli M., Mastinu A., Maccarinelli G, & Memo M. (2020). Brain Structural and Functional Alterations in Mice Prenatally Exposed to LPS Are Only Partially Rescued by Anti-Inflammatory Treatment. Brain Sciences, 10(9), 620.
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3

Courtship Ultrasonic Vocalizations in Mice

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Female mice in estrus were identified using vaginal lavage, followed by cytological staining (Giemsa solution, Polysciences) and visual assessment. Male mice were habituated to the experimental arena (40 × 20 cm) for 3 min. Next, an unfamiliar female mouse in estrus was added to the arena, and the mice were allowed to interact freely for 3 min. Ultrasonic vocalizations (USVs) between 40 and 250 Hz produced by the male mice were recoded using a preamplifier (UltraSoundGate 416 H, Avisoft Bioacoustics) connected to a microphone (UltraSoundGate CM16, Avisoft Bioacoustics). The total number of USVs and their duration was analyzed using SASLabPro (Avisoft Bioacoustics).
Sledziowska M., Kalbassi S, & Baudouin S.J. (2020). Complex Interactions between Genes and Social Environment Cause Phenotypes Associated with Autism Spectrum Disorders in Mice. eNeuro, 7(4), ENEURO.0124-20.2020.
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4

Ultrasonic Vocalization Analysis in Mice

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The Ultrasonic vocalization (USV) test18 (link) was performed in mice at P7 because the number, duration, and average peak frequency of USVs had a peak around P782 (link). Both male and female pups at P7 were isolated from their mothers at random and gently placed into a container (plastic cylinder, 5 cm high, 5 cm in diameter). USV was recorded for 5 min from the time of isolation. USV emission was monitored by an ultrasonic microphone (UltraSoundGate CM 16; Avisoft Bioacoustics, Berlin, Germany). The isolation container and microphone were placed in a sound attenuating box (63.5 × 42 × 37 cm). The microphone (sensitive to frequencies of 15–250 kHz) was connected to a personal computer with a sampling rate of 250 kHz in 16-bit format by Avisoft RECORDER (version 4.2.16; Avisoft Bioacoustics) via a USB recording interface (UltraSoundGate 116Hb; Avisoft Bioacoustics, Berlin, Germany).
Sacai H., Sakoori K., Konno K., Nagahama K., Suzuki H., Watanabe T., Watanabe M., Uesaka N, & Kano M. (2020). Autism spectrum disorder-like behavior caused by reduced excitatory synaptic transmission in pyramidal neurons of mouse prefrontal cortex. Nature Communications, 11, 5140.
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5

Analyzing Male Mouse Ultrasonic Vocalization

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We recorded ultrasonic vocalization (USV) emitted by the male mice exposed to a female mouse in estrus (1, 2). All male mice were first habituated for 3 min to the arena. Following the habituation, an unfamiliar female mouse in estrus was added to the same arena for 3 min. During this time the mice could interact with each other freely in the arena. The male vocalization behavior was recorded by a preamplifier (UltraSoundGate 416 H, Avisoft Bioacoustics) connected microphone (UltraSoundGate CM16, Avisoft Bioacoustics) placed above the arena. As previously described frequencies were recorded and analyzed for the total number of emitted calls and the total time spent calling using SASLabPro (Avisoft Bioacoustics).
Bachmann S.O., Sledziowska M., Cross E., Kalbassi S., Waldron S., Chen F., Ranson A, & Baudouin S.J. (2019). Behavioral training rescues motor deficits in Cyfip1 haploinsufficiency mouse model of autism spectrum disorders. Translational Psychiatry, 9, 29.
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6

Ultrasonic Vocalizations in DNMT3A Mutant Mice

Cited 3 times
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A total of 76 DNMT3AKO/+ (n = 30, 16 male and 14 female) and litter-matched WT (n = 46, 25 male and 21 female) mice were used for ultrasonic pup vocalization (USV) recording and analyzed as previously described (Barnes et al., 2017 (link); Dougherty et al., 2013 (link); Holy and Guo, 2005 (link)). Dams were removed from the nest for a 10-minute acclimation, and individual pups had their body temperature measured using an infrared laser thermometer. Pups were then removed from their nest and placed in a dark, enclosed chamber. Ultrasonic vocalizations were recorded for 3 minutes with an Avisoft UltraSoundGate CM16 microphone and 416H amplifier using Avisoft Recorder software (gain = 6 dB, 16 bits, sampling rate = 250 kHz). Pups were then weighed and returned to their nest and littermates. All mice were recorded at postnatal days 5, 7, and 9, and on either day 11 or 15. Frequency sonograms were prepared and analyzed in MATLAB (frequency range = 40 kHz to 120 kHz, FFT size = 256, overlap = 50%) with individual syllables identified and counted according to previously published methods (Dougherty et al., 2013 (link); Holy and Guo, 2005 (link)). Significance was assessed using a within-subjects repeated-measures ANOVA over time points 5–9, as these were when there was data from all experimental subjects, and these are optimal testing times where number of calls was highest.
Christian D.L., Wu D.Y., Martin J.R., Moore J.R., Liu Y.R., Clemens A.W., Nettles S.A., Kirkland N.M., Papouin T., Hill C.A., Wozniak D.F., Dougherty J.D, & Gabel H.W. (2020). DNMT3A Haploinsufficiency Results in Behavioral Deficits and Global Epigenomic Dysregulation Shared across Neurodevelopmental Disorders. Cell reports, 33(8), 108416.
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7

Ultrasonic Vocalizations in DNMT3A Mutant Mice

Cited 3 times
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A total of 76 DNMT3AKO/+ (n = 30, 16 male and 14 female) and litter-matched WT (n = 46, 25 male and 21 female) mice were used for ultrasonic pup vocalization (USV) recording and analyzed as previously described (Barnes et al., 2017 (link); Dougherty et al., 2013 (link); Holy and Guo, 2005 (link)). Dams were removed from the nest for a 10-minute acclimation, and individual pups had their body temperature measured using an infrared laser thermometer. Pups were then removed from their nest and placed in a dark, enclosed chamber. Ultrasonic vocalizations were recorded for 3 minutes with an Avisoft UltraSoundGate CM16 microphone and 416H amplifier using Avisoft Recorder software (gain = 6 dB, 16 bits, sampling rate = 250 kHz). Pups were then weighed and returned to their nest and littermates. All mice were recorded at postnatal days 5, 7, and 9, and on either day 11 or 15. Frequency sonograms were prepared and analyzed in MATLAB (frequency range = 40 kHz to 120 kHz, FFT size = 256, overlap = 50%) with individual syllables identified and counted according to previously published methods (Dougherty et al., 2013 (link); Holy and Guo, 2005 (link)). Significance was assessed using a within-subjects repeated-measures ANOVA over time points 5–9, as these were when there was data from all experimental subjects, and these are optimal testing times where number of calls was highest.
Christian D.L., Wu D.Y., Martin J.R., Moore J.R., Liu Y.R., Clemens A.W., Nettles S.A., Kirkland N.M., Papouin T., Hill C.A., Wozniak D.F., Dougherty J.D, & Gabel H.W. (2020). DNMT3A Haploinsufficiency Results in Behavioral Deficits and Global Epigenomic Dysregulation Shared across Neurodevelopmental Disorders. Cell reports, 33(8), 108416.
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8

Ultrasonic Vocalization Measurement in Pups

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USVs were recorded as described previously [40] [41] [42] . Pups were individually removed from the home cage and isolated from their mother and littermates. Each pup was placed in a plastic cup in a soundproof room and USVs were recorded for 5 min using a microphone (Ultra-SoundGate CM16; Avisoft Bioacoustics, Berlin, Germany).
After the recording, the pups were returned to the home cage. The USVs were recorded at PDs 6, 7, 10, 14, 17, and 21, and analyzed using Avisoft-SAS Lab Pro (Avisoft Bioacoustics). The number of calls was counted for 3 min after 60 s of acclimation (i.e. between 60 s and 240 s after isolation). Calls with a frequency of 40-120 kHz and du-ration of >10 ms were counted as USVs.
Shigemori T., Sakai A., Takumi T., Itoh Y, & Suzuki H. (2015). Altered Microglia in the Amygdala Are Involved in Anxiety-related Behaviors of a Copy Number Variation Mouse Model of Autism. Journal of Nippon Medical School = Nippon Ika Daigaku zasshi, 82(2).
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