Cd11c bv711

Manufactured by BioLegend

Sourced in United States

CD11c-BV711 is a fluorochrome-conjugated antibody that recognizes the CD11c antigen, which is expressed on the surface of dendritic cells and other myeloid cells. This product is intended for use in flow cytometry applications.

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Close spelling variants of this product

CD11c-PE or BV711 (HL3, (2 citations)

6 protocols using cd11c bv711

Comprehensive Immunophenotyping by Flow Cytometry

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All cells were stained for viability using the Zombie Fixable Viability Kit (Biolegend), incubated with anti-CD16/32 Fc-block (BioXcell), and stained with the indicated antibodies: CD19 APC, CD3 APC-H7 or BV450, CD4 AF700, CD8 V500, CCR7 PE-CF594, CD69 BV785, CCR5 PE, CD103 FITC, (BD Biosciences) CD38 PE-Cy7, CD11c BV711, CD14 BV650, CD45RA BV605 (Biolegend). Stained samples were run on an LSRII flow cytometer, data acquired using FACS DIVA software (BD Biosciences) and analyzed using FlowJo software (TreeStar, Inc., Ashland, Oregon).

Swaims-Kohlmeier A., Haaland R.E., Haddad L.B., Sheth A.N., Evans-Strickfaden T., Lupo L.D., Cordes S., Aguirre A.J., Lupoli K.A., Chen C.Y., Ofotukun I., Hart C.E, & Kohlmeier J.E. (2016). Progesterone levels associate with a novel population of CCR5+ CD38+ CD4 T cells resident in the genital mucosa with lymphoid trafficking potential. Journal of immunology (Baltimore, Md. : 1950), 197(1), 368-376.

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Comprehensive Immune Cell Profiling in Mouse Lungs

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BMDCs and/or whole lung lysates were incubated with Zombie UV Fixable Viability Kit (BioLegend, San Diego, CA) at room temperature for 15 minutes. Cells were washed with FACS rinsing buffer, pelleted, and incubated with anti-mouse CD16/32 (BioLegend) for 15 minutes at 4 °C to block non-specific Ig binding. Following subsequent washing and centrifugation, cells were incubated with antibody cocktails containing some or all of the following markers: CD11c BV711, CD80 BV650, CD64 PerCP/Cy5.5, Ly6C BV605, CCR-7 APC, MHC-II (I-A/I-E) BV 421, Ly6G BV650, CD40 PE/Cy7,CD4 APC/Cy7, CD3 APC, and CD8α PerCP/Cy5.5 (BioLegend); CD86 BUV395, CD11b BV480, Siglec-F APC-R700, CD45 BV805, CD103 PE-CF594, and CD24 BUV 737 (BD Biosciences, San Jose, CA). Cells were analyzed using the BD LSRFortessa™ (BD Biosciences). Cell aggregates were removed by gating single cells on the forward light scatter (FSC-H vs FSC-A). Dead cell and debris were excluded before gating for myeloid innate immune cells and/or T-cell markers. Fluorescence minus one (FMO) controls were used to set up gating strategies used in these experiments. Data was analyzed using FlowJo software v10.7.1 (Tree Star Inc.).

Hall S.C., Smith D.R., Dyavar S.R., Wyatt T.A., Samuelson D.R., Bailey K.L, & Knoell D.L. (2021). Critical Role of Zinc Transporter (Zip8) in Myeloid Innate Immune Cell Function and the Host Response against Bacterial Pneumonia. Journal of immunology (Baltimore, Md. : 1950), 207(5), 1357-1370.

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Immunophenotyping of Activated BMDCs and Macrophages

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BMDC and MØ incubated with or without the peptides (5, 10 or 20 µg/mL) were washed with FACS buffer twice. Then, supernatants were removed and FACS buffer with Live/Dead – BV450 (BD Horizon); MHC-II – PE-Cy7 (clone: M5/114.15.2; Biolegend); F4/80 – FITC (clone: BM8; Biolegend); CD11c – BV711 (clone: N418; Biolegend); CD80 – PE (clone: 16-10A1; eBioscience); CD86 – APC (clone: GK1.5; Biolegend) were added for immunophenotyping and assessment of activation. The cells were incubated for 30 min at 4°C in the dark, washed 2X with FACS buffer and fixed with 1% paraformaldehyde. Sample acquisition was performed on a flow cytometer FACS BD LSRII housed in the Flow Cytometry Core Facility at the Einstein.

Silva L.B., Taira C.L., Cleare L.G., Martins M., Junqueira M., Nosanchuk J.D, & Taborda C.P. (2021). Identification of Potentially Therapeutic Immunogenic Peptides From Paracoccidioides lutzii Species. Frontiers in Immunology, 12, 670992.

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Comprehensive Flow Cytometry Analysis of Murine Lung Immune Cells

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BAL leukocytes, tissue-isolated leukocytes or in vitro-stimulated cDCs were stained with TruStain FcX anti-mouse CD16/32 antibody (BioLegend, San Diego, CA, USA) and CD45-APC-Cy7, CD103-PE, CD11b-PeCy7, CD11c-BV711, I-A/I-E (MHC II)-Pacific Blue, I-A/I-E (MHC II)-PERCP, CD172a (Sirpα)-APC-Cy7, CD19-biotin, CD90.2-biotin, IRF4-PE, Ly-6G-PE, XCR1-APC, CD8α-APC-Cy7, Lineage antibody cocktail-Pacific Blue, CD135 (FLT3)-biotin, Streptavidin-PERCP (BioLegend), NK1.1-biotin (ablab, Vancouver, BC, CA), CD11c-APC, Siglec-F-BV711 (BD Biosciences, San Jose, USA), IRF8-APC (Miltenyi Biotec, Bergisch Gladbach, Allemagne) and Streptavidin-Pe-Cy7 (eBioscience, Thermo Fisher Scientific), GM–CSFRα-APC (R&D system, Minneapolis, MN, USA). Total, neutrophils and macrophages BAL number were determined using precision count beads (BioLegend). Intracellular staining was performed using the True-Nuclear™ Transcription Factor Buffer Set (BioLegend) according to the manufacturer’s instructions. Cells were analyzed using a BD LSR Fortessa cytometer (BD Biosciences) and FlowJo software V10 (BD, Franklin Lakes, NJ, USA). Mean fluorescence intensity (MFI) data were analyzed as Δ MFI, which corresponds to the MFI of the antigen-positive population minus the MFI of the fluorescence minus one (FMO) control of this population.

Brassard J., Roy J., Lemay A.M., Beaulieu M.J., Bernatchez E., Veillette M., Duchaine C, & Blanchet M.R. (2021). Exposure to the Gram-Negative Bacteria Pseudomonas aeruginosa Influences the Lung Dendritic Cell Population Signature by Interfering With CD103 Expression. Frontiers in Cellular and Infection Microbiology, 11, 617481.

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Isolation and Characterization of Uterine Immune Cells

Cited 1 time

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Uterine samples consisting of maternal decidua and myometrium were
collected 6 h after ultrapure LPS challenge (or saline) on d16 of gestation
from WT and BAFF reporter (RFP^{48 (link)}) mice. Immune cells were isolated using enzymatic
digestion. Briefly, uterine tissue was dissected and finely minced. Tissue
was digested using Liberase TM (21 µg/mL, Roche) and Dnase I (8.8
µg/mL, Roche) in DMEM containing HEPES and 2% BSA. Tissue was
incubated at 37°C for 30 min at 220 RPM. After digestion, cells were
filtered through a 100 µM strainer and centrifuged at 800 g for 5
min. Following red blood cell lysis, single cell suspensions were stained
with Live/Dead (Zombie UV Dye: Biolegend) and directly conjugated monoclonal
antibodies to CD45-PE-Dazzle594 (Biolegend, 104), F4/80-AF700 (Biolegend,
BM8), TCRb-APC-ef780 (Invitrogen, H57–597), CD11b-ef450 (Biolegend,
17A2), CD8-BV510 (Biolegend, 53–6.7), B220-BV605 (Biolegend,
RA3–6B2), NK1.1-FITC (Biolegend, PK136), CD11c-BV711 (Biolegend,
N418), Ly6G-APC (Invitrogen, RB6–8C5), Ly6C-PerCP-Cy5.5 (eBioscience,
HK1.4). Data was collected using LSR Fortessa (BD) and analyzed using FlowJo
X software (vX10).

Smith H.C., Kuo S.R., Backus J.W., Harris S.G., Sparks C.E, & Sparks J.D. (1991). In vitro apolipoprotein B mRNA editing: identification of a 27S editing complex.. Proceedings of the National Academy of Sciences of the United States of America, 88(4), 1489-1493.

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Multiparametric Immune Cell Profiling

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The following antibodies were used to identify cell subsets: Ly6C-FITC (AL-21, BD Biosciences), F4/80-APC eFluor660 (BM8, eBioscience), Siglec-F-PE or AR700 (E50-2440, BD Biosciences), CD11c-PE or BV711 (HL3, BioLegend), Ly6G-PerCP eFluor710 or V450 (1A8, eBioscience or BD Biosciences), CD45.2-APC/Fire750 or V450 (104, BioLegend or eBioscience), CD45.1-PE (A20, eBioscience), and CD11c-BV711 (N418, BioLegend). Dead cells were excluded from analyses using Fixable Viability Dye APC BV506 (eBioscience).
Surface staining: Cells were treated with anti-CD16/CD32 Fc receptor blocking antibody (clone 2.4G2) in 1x PBS (1% FBS) for 10 minutes on ice. Cells were then centrifuged and resuspended in 1x PBS (1% FBS) containing antibodies and incubated for 15 minutes on ice. Cells were washed with 1x PBS then incubated with Fixable Viability Dye diluted in 1x PBS for 15 minutes on ice.
Cells were washed, then fixed with 1% paraformaldehyde for 15 minutes on ice. Samples were acquired using an Attune NxT Acoustic Focusing Cytometer with Attune Software.
Analyses were performed using FlowJo v10 software (Tree Star, Inc.). Gate placement was determined using fluorescence minus one and unstained control samples.

Crane M.J., Xu Y., Monaghan S.F., Hall B.M., Albina J.E., Henry W.P., Tran H.L., Chhabria K., Jordon A.R.D., Carlsen L., & Jamieson A.M. (2020). Pulmonary infection interrupts acute cutaneous wound healing through disruption of chemokine signals.

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