Pstat3 antibodies

IHC analysis for PCNA, pAKT, and pSTAT3 proteins was performed on formalin fixed, paraffin embedded orthotopic xenograft tumors (5 micron sections). Briefly, the tumor tissues were deparaffinized, rehydrated, treated with 0.3% hydrogen peroxide and processed for antigen retrieval using a heat-induced technique. Following blocking with background sniper (Biocare Medical, Concord, CA), the samples were processed for staining with PCNA, pAKT, and pSTAT3 antibodies (Cell Signaling Technologies, Danvers, MA). The expression of these proteins was detected using a MACH 4 Universal HRP Polymer detection kit (Biocare Medical) and 3, 9-diaminobenzidine (DAB substrate kit, Vector Laboratories, Burlingame, CA). The slides were counterstained with hematoxylin, dehydrated, mounted with VectaMount (Vector Laboratories) and visualized using an Olympus BX 41 Microscope (Olympus Corporation, Tokyo, Japan).

Isoprenaline was obtained from He Feng (Shanghai, China). α-SMA and collagen I antibodies for were purchased from Boster (Wuhan, China), CADM1, p-STAT3 antibodies were obtained from Cell Signaling (Beverly, MA, USA). The primers of CADM1, STAT3, α-SMA, collagen I and β-actin were produced by Shanghai Sangong Biological and Technological Company (Shanghai, China). Immunohistochemical kit was obtained from the Zhongshan Biotechnology Corporation (Beijing, China). SYBR Green Real Master Mix and Reverse transcription reaction Mix were purchased from Fermentas (Ontario, Canada). Goat anti-rabbit IgG horse radish peroxidase (HRP), rabbit anti-goat IgG HRP, goat anti-mouse IgG HRP were obtained from Santa Cruz (California, USA).